ࡱ>  y{pqrstuvwxg RbjbjVV2r<r<V ,,,,,@@@8x@W8,8,8,8,8,l-l-l-WWWWWWW$3Y[H(W9,r.l-l-r.r.(W,,8,8,4aWVVVr.&,8,,8,WVr.WVVV8,:f7CUVVwW0WV-^U-^VV-^,VPl--V- -l-l-l-(W(WnVl-l-l-Wr.r.r.r.-^l-l-l-l-l-l-l-l-l-, : pEASY-T1 Simple Complete Sequence AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTGCCCTTAGGGCAGCTTCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCCCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG M13F5'-GTAAAACGACGGCCAGT-3' vU_SGJ101-01 M13R5'-CAGGAAACAGCTATGAC-3' vU_SGJ101-11 LacZa fragment: bases 1-448 M13 reverse priming site: bases 205-221 T7 promoter/priming site: bases 264-281 M13 Forward (-20) priming site: bases 289-304 f1 origin: bases 446-883 Kanamycin resistance ORF: bases 1217-2011 Ampicillin resistance ORF: bases 2029-2889 pUC origin: bases 3034-3707 pEASY-T1 Simple Restriction Map NEWMOL Cutters M.CviQII (348)rar25, 46, 72, 123, 127, 145, 152, 159, 166, 201245, 255, 261, 288, 302, 343, 352, 368, 413, 513535, 539, 544, 548, 553, 557, 561, 570, 578, 592606, 610, 618, 624, 631, 642, 657, 674, 706, 715735, 750, 756, 774, 785, 794, 798, 803, 814, 823853, 864, 875, 886, 1023, 1034, 1062, 1105, 1146, 11501214, 1249, 1260, 1277, 1312, 1328, 1356, 1360, 1443, 14601523, 1538, 1542, 1595, 1616, 1656, 1673, 1712, 1730, 17391769, 1795, 1825, 1835, 1913, 1936, 1940, 1946, 1962, 19691984, 1994, 2006, 2039, 2046, 2059, 2063, 2067, 2071, 20802086, 2093, 2099, 2176, 2194, 2202, 2220, 2237, 2246, 22962316, 2328, 2340, 2362, 2433, 2437, 2474, 2478, 2504, 25092620, 2624, 2630, 2683, 2691, 2698, 2713, 2750, 2790, 28712906, 2910, 2920, 2931, 2938, 2943, 2950, 2974, 2986, 30023013, 3019, 3059, 3071, 3075, 3096, 3131, 3135, 3158, 31623180, 3209, 3240, 3249, 3271, 3275, 3325, 3339, 3390, 34083472, 3544, 3568, 3586, 3595, 3601, 3613, 3624, 3669, 36793687, 3698, 3704, 3712, 3716, 3735, 3759, 3779, 18, 8396, 116, 181, 188, 211, 237, 274, 322, 391, 435442, 470, 589, 596, 651, 726, 760, 766, 808, 839846, 859, 872, 882, 896, 906, 910, 916, 925, 940961, 988, 992, 1000, 1006, 1011, 1075, 1079, 1085, 11121120, 1130, 1135, 1159, 1183, 1194, 1206, 1226, 1230, 12681367, 1390, 1394, 1406, 1475, 1482, 1505, 1550, 1554, 16261630, 1643, 1647, 1664, 1697, 1724, 1742, 1763, 1801, 18141907, 1922, 2003, 2013, 2018, 2022, 2026, 2034, 2107, 21182124, 2134, 2153, 2167, 2188, 2197, 2212, 2232, 2242, 22882322, 2335, 2353, 2382, 2386, 2412, 2453, 2460, 2464, 25212527, 2532, 2536, 2547, 2554, 2599, 2614, 2659, 2668, 27252732, 2740, 2785, 2820, 2839, 2843, 2860, 2881, 2903, 29342953, 2967, 2995, 3010, 3026, 3043, 3052, 3065, 3101, 31053109, 3146, 3168, 3188, 3201, 3244, 3316, 3342, 3361, 33803412, 3416, 3430, 3435, 3451, 3458, 3462, 3478, 3483, 34873507, 3521, 3527, 3537, 3541, 3554, 3604, 3639, 3648, 37633767, 3791, 3799, 3808, 3816, 3820, 3824, 3829M.CviPI (299)gc4, 15, 22, 32, 40, 54, 57, 61, 86, 9097, 104, 121, 134, 143, 157, 164, 189, 216, 233238, 241, 250, 253, 263, 294, 331, 349, 354, 357372, 376, 380, 388, 396, 400, 409, 425, 430, 439449, 459, 462, 471, 476, 493, 498, 506, 515, 519527, 533, 542, 563, 572, 576, 590, 604, 629, 637685, 691, 711, 817, 827, 847, 869, 891, 899, 902912, 928, 936, 947, 968, 976, 997, 1001, 1014, 10211025, 1032, 1041, 1047, 1057, 1072, 1076, 1089, 1093, 10991122, 1127, 1144, 1152, 1155, 1170, 1217, 1239, 1243, 12551258, 1272, 1280, 1291, 1307, 1310, 1318, 1321, 1332, 13391347, 1384, 1398, 1408, 1411, 1416, 1425, 1429, 1439, 14481453, 1458, 1477, 1491, 1494, 1502, 1509, 1515, 1540, 15461567, 1573, 1578, 1581, 1584, 1587, 1593, 1603, 1610, 16271637, 1644, 1648, 1666, 1701, 1710, 1714, 1720, 1732, 17371744, 1748, 1752, 1760, 1784, 1789, 1793, 1797, 1820, 18231847, 1851, 1861, 1868, 1882, 1888, 1903, 1911, 1916, 19191927, 1934, 1944, 1957, 1960, 1971, 1974, 1981, 1991, 20552073, 2076, 2083, 2097, 2111, 2130, 2148, 2182, 2206, 22332249, 2257, 2280, 2285, 2291, 2302, 2305, 2357, 2371, 23922397, 2400, 2424, 2427, 2465, 2472, 2480, 2506, 2528, 25382555, 2570, 2577, 2583, 2594, 2611, 2628, 2636, 2661, 26732687, 2694, 2703, 2707, 2718, 2733, 2741, 2752, 2763, 27662774, 2786, 2826, 2856, 2868, 2882, 3027, 3080, 3091, 30943098, 3116, 3123, 3137, 3149, 3178, 3184, 3189, 3221, 32323255, 3260, 3273, 3278, 3297, 3300, 3303, 3310, 3363, 33683376, 3394, 3401, 3406, 3413, 3446, 3452, 3463, 3470, 34893508, 3511, 3530, 3542, 3558, 3589, 3605, 3622, 3635, 36403654, 3658, 3663, 3666, 3684, 3691, 3695, 3702, 3720, 37563768, 3777, 3781, 3784, 3787, 3800, 3805, 3817, 3825M.SssI (256)cg5, 14, 21, 31, 35, 42, 64, 76, 87, 98103, 162, 167, 190, 232, 262, 277, 296, 299, 309312, 332, 348, 371, 381, 389, 399, 404, 408, 426440, 448, 460, 463, 472, 477, 492, 499, 505, 520528, 532, 541, 562, 567, 571, 574, 584, 599, 620635, 643, 677, 690, 702, 710, 720, 732, 786, 819825, 868, 892, 913, 920, 935, 943, 955, 996, 10021042, 1058, 1077, 1082, 1100, 1154, 1171, 1211, 1216, 12421253, 1257, 1278, 1305, 1320, 1323, 1330, 1340, 1348, 13521371, 1380, 1404, 1412, 1421, 1433, 1436, 1440, 1449, 14611464, 1478, 1503, 1511, 1548, 1579, 1582, 1592, 1601, 16171628, 1636, 1641, 1645, 1651, 1657, 1668, 1677, 1695, 17131722, 1731, 1745, 1755, 1758, 1761, 1770, 1773, 1785, 17991822, 1839, 1849, 1862, 1867, 1883, 1894, 1917, 1920, 19331941, 1950, 1956, 1959, 1965, 1970, 1975, 1980, 1990, 20012049, 2054, 2074, 2110, 2151, 2165, 2183, 2205, 2210, 22172258, 2271, 2279, 2282, 2297, 2301, 2304, 2365, 2425, 24462450, 2458, 2471, 2505, 2514, 2524, 2549, 2552, 2556, 25662590, 2595, 2612, 2634, 2662, 2688, 2692, 2701, 2735, 27422751, 2793, 2798, 2812, 2815, 2841, 2855, 3006, 3016, 30283038, 3081, 3115, 3124, 3139, 3166, 3190, 3223, 3259, 32723311, 3319, 3329, 3345, 3355, 3364, 3369, 3373, 3382, 33913414, 3418, 3428, 3447, 3464, 3469, 3476, 3490, 3502, 35093518, 3531, 3535, 3557, 3581, 3588, 3606, 3609, 3625, 36363653, 3662, 3675, 3721, 3747, 3755, 3776, 3780, 3783, 37893793, 3797, 3801, 3806, 3818, 3826M.CviAIV (254)rgcb7, 23, 41, 58, 122, 144, 165, 217, 234, 239254, 264, 295, 350, 373, 397, 410, 431, 452, 507522, 530, 543, 564, 573, 591, 605, 686, 692, 712828, 848, 900, 929, 969, 977, 1022, 1033, 1048, 10941102, 1123, 1145, 1156, 1256, 1273, 1281, 1308, 1322, 13331350, 1417, 1426, 1454, 1492, 1568, 1585, 1594, 1604, 16671711, 1717, 1733, 1738, 1821, 1848, 1869, 1889, 1912, 19281935, 1958, 1982, 1992, 2056, 2112, 2207, 2281, 2303, 24282466, 2473, 2507, 2529, 2539, 2629, 2690, 2695, 2704, 27342775, 2787, 2857, 3117, 3150, 3179, 3222, 3233, 3261, 32743298, 3377, 3402, 3407, 3453, 3466, 3471, 3543, 3559, 35903641, 3655, 3667, 3685, 3696, 3757, 3769, 3778, 3788, 541, 58, 87, 98, 122, 144, 165, 190, 217, 239254, 295, 332, 377, 389, 397, 431, 440, 460, 472477, 499, 520, 528, 577, 591, 605, 686, 828, 848892, 900, 913, 929, 969, 977, 1002, 1022, 1033, 10421048, 1058, 1077, 1094, 1100, 1123, 1145, 1171, 1256, 12731281, 1308, 1333, 1340, 1348, 1412, 1417, 1426, 1440, 14541478, 1492, 1503, 1568, 1582, 1604, 1628, 1645, 1667, 17111721, 1738, 1745, 1761, 1785, 1821, 1848, 1862, 1883, 18891912, 1917, 1928, 1975, 2183, 2258, 2428, 2466, 2529, 25392556, 2612, 2629, 2662, 2695, 2704, 2734, 2742, 2775, 27873028, 3124, 3150, 3179, 3190, 3222, 3233, 3298, 3311, 33643369, 3377, 3402, 3407, 3414, 3447, 3453, 3464, 3490, 35093531, 3543, 3606, 3636, 3641, 3667, 3685, 3696, 3769, 37883801, 3806, 3818, 3826M.CviJI (254)rgcb7, 23, 41, 58, 122, 144, 165, 217, 234, 239254, 264, 295, 350, 373, 397, 410, 431, 452, 507522, 530, 543, 564, 573, 591, 605, 686, 692, 712828, 848, 900, 929, 969, 977, 1022, 1033, 1048, 10941102, 1123, 1145, 1156, 1256, 1273, 1281, 1308, 1322, 13331350, 1417, 1426, 1454, 1492, 1568, 1585, 1594, 1604, 16671711, 1717, 1733, 1738, 1821, 1848, 1869, 1889, 1912, 19281935, 1958, 1982, 1992, 2056, 2112, 2207, 2281, 2303, 24282466, 2473, 2507, 2529, 2539, 2629, 2690, 2695, 2704, 27342775, 2787, 2857, 3117, 3150, 3179, 3222, 3233, 3261, 32743298, 3377, 3402, 3407, 3453, 3466, 3471, 3543, 3559, 35903641, 3655, 3667, 3685, 3696, 3757, 3769, 3778, 3788, 541, 58, 87, 98, 122, 144, 165, 190, 217, 239254, 295, 332, 377, 389, 397, 431, 440, 460, 472477, 499, 520, 528, 577, 591, 605, 686, 828, 848892, 900, 913, 929, 969, 977, 1002, 1022, 1033, 10421048, 1058, 1077, 1094, 1100, 1123, 1145, 1171, 1256, 12731281, 1308, 1333, 1340, 1348, 1412, 1417, 1426, 1440, 14541478, 1492, 1503, 1568, 1582, 1604, 1628, 1645, 1667, 17111721, 1738, 1745, 1761, 1785, 1821, 1848, 1862, 1883, 18891912, 1917, 1928, 1975, 2183, 2258, 2428, 2466, 2529, 25392556, 2612, 2629, 2662, 2695, 2704, 2734, 2742, 2775, 27873028, 3124, 3150, 3179, 3190, 3222, 3233, 3298, 3311, 33643369, 3377, 3402, 3407, 3414, 3447, 3453, 3464, 3490, 35093531, 3543, 3606, 3636, 3641, 3667, 3685, 3696, 3769, 37883801, 3806, 3818, 3826M.HindI (96)cac94, 114, 176, 186, 272, 314, 481, 486, 501, 628667, 682, 745, 946, 1029, 1265, 1325, 1383, 1423, 14571508, 1775, 1811, 1845, 1857, 1896, 1943, 2051, 2116, 21472155, 2255, 2396, 2414, 2558, 2738, 2744, 2867, 2965, 30083127, 3217, 3295, 3308, 3321, 3393, 3449, 3618, 3739, 37653814, 63, 125, 136, 150, 204, 290, 359, 402, 511566, 639, 676, 731, 772, 919, 1241, 1293, 1432, 14711535, 1622, 1650, 2101, 2150, 2235, 2311, 2342, 2349, 24212482, 2562, 2681, 2768, 2811, 2873, 3022, 3113, 3235, 32573396, 3426, 3468, 3534, 3592, 3706CviJI (75)rgcy41, 58, 122, 144, 165, 217, 239, 254, 295, 377397, 431, 577, 591, 605, 686, 828, 848, 900, 929969, 977, 1022, 1033, 1048, 1094, 1123, 1145, 1256, 12731281, 1308, 1333, 1417, 1426, 1454, 1492, 1568, 1585, 16041667, 1711, 1721, 1738, 1821, 1848, 1889, 1912, 1928, 24282466, 2529, 2539, 2629, 2695, 2704, 2734, 2775, 2787, 31503179, 3222, 3233, 3298, 3377, 3402, 3407, 3453, 3543, 36413667, 3685, 3696, 3769, 3788M.CviTI (75)rgcy41, 58, 122, 144, 165, 217, 239, 254, 295, 377397, 431, 577, 591, 605, 686, 828, 848, 900, 929969, 977, 1022, 1033, 1048, 1094, 1123, 1145, 1256, 12731281, 1308, 1333, 1417, 1426, 1454, 1492, 1568, 1585, 16041667, 1711, 1721, 1738, 1821, 1848, 1889, 1912, 1928, 24282466, 2529, 2539, 2629, 2695, 2704, 2734, 2775, 2787, 31503179, 3222, 3233, 3298, 3377, 3402, 3407, 3453, 3543, 36413667, 3685, 3696, 3769, 3788M.NgoMXV (56)gcchr40, 61, 90, 134, 143, 294, 331, 380, 685, 9761032, 1057, 1291, 1429, 1439, 1491, 1502, 1737, 1888, 19161927, 2285, 2611, 2707, 2826, 3178, 3232, 3376, 3684, 36959, 236, 375, 399, 433, 518, 532, 830, 931, 10921158, 1352, 1613, 1719, 1735, 1755, 1792, 2430, 2573, 27773263, 3306, 3404, 3561, 3643, 3657AciI (44)ccgc88, 191, 461, 475, 914, 1059, 1415, 1479, 1580, 18631918, 2075, 2184, 2261, 2426, 2663, 2754, 3125, 3370, 34913510, 3637, 3665, 3827, 22, 32, 400, 506, 533, 9361155, 1258, 1321, 1823, 1868, 1934, 1960, 2305, 2472, 31163260, 3756, 3777, 3784BthCI (34)gcngc57, 241, 253, 357, 430, 462, 476, 498, 902, 11551258, 1310, 1321, 1411, 1453, 1494, 1581, 1587, 1823, 19191960, 1974, 2076, 2305, 2400, 2427, 2766, 3094, 3300, 33683511, 3666, 3784, 3805Fnu4HI (34)gcngc57, 241, 253, 357, 430, 462, 476, 498, 902, 11551258, 1310, 1321, 1411, 1453, 1494, 1581, 1587, 1823, 19191960, 1974, 2076, 2305, 2400, 2427, 2766, 3094, 3300, 33683511, 3666, 3784, 3805HhaI (30)gcgc6, 34, 99, 427, 451, 464, 473, 495, 521, 529893, 1003, 1101, 1172, 1341, 1349, 1413, 1450, 1716, 17461976, 2259, 2596, 2689, 3082, 3191, 3365, 3465, 3532, 3802HinP1I (30)gcgc6, 34, 99, 427, 451, 464, 473, 495, 521, 529893, 1003, 1101, 1172, 1341, 1349, 1413, 1450, 1716, 17461976, 2259, 2596, 2689, 3082, 3191, 3365, 3465, 3532, 3802M.HhaI (30)gcgc6, 34, 99, 427, 451, 464, 473, 495, 521, 529893, 1003, 1101, 1172, 1341, 1349, 1413, 1450, 1716, 17461976, 2259, 2596, 2689, 3082, 3191, 3365, 3465, 3532, 3802M.HinP1I (30)gcgc6, 34, 99, 427, 451, 464, 473, 495, 521, 529893, 1003, 1101, 1172, 1341, 1349, 1413, 1450, 1716, 17461976, 2259, 2596, 2689, 3082, 3191, 3365, 3465, 3532, 3802M.HpyAVIII (30)gcgc6, 34, 99, 427, 451, 464, 473, 495, 521, 529893, 1003, 1101, 1172, 1341, 1349, 1413, 1450, 1716, 17461976, 2259, 2596, 2689, 3082, 3191, 3365, 3465, 3532, 3802Sth132I (28)cccg89, 479, 601, 1438, 1480, 1513, 2284, 2817, 3331, 33753384, 3583, 31, 76, 399, 532, 584, 955, 1352, 17551894, 1965, 2210, 2271, 2634, 2793, 3038, 3476BstKTI (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143ChaI (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143DpnI (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.AacDam (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.AlaK2I (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.AvaV (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.AvaVI (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.BspKT6I (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.CviAI (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.DpnIIA (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.DpnIIB (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.Eco67Dam (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.EcoKDam (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.EcoP1Dam (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.EcoT1Dam (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.EcoT2Dam (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.EcoT4Dam (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.EsaLHCI (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.HindDam (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.HinHP1Dam (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.Hpy99VI (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.HpyAIII (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.LlaDCHIA (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.LlaDCHIB (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.LlaKR2I (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.MboIA (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.MboIB (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.PgiI (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.PhiCh1I (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.Sau3AI (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.SmaII (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.Sth368I (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.Sty1344Dam (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.StyDam (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143M.TpaI (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143MboI (24)gatc407, 1163, 1180, 1186, 1191, 1210, 1521, 1599, 1680, 16891767, 2138, 2174, 2191, 2449, 2495, 2513, 2854, 2959, 29713049, 3057, 3068, 3143Cac8I (23)gcnngc61, 90, 376, 400, 519, 533, 576, 1001, 1025, 10761093, 1243, 1429, 1648, 1714, 1748, 1793, 1851, 2707, 30983658, 3695, 3781HpyF10VI (23)gcnnnnnnngc15, 99, 143, 250, 409, 439, 471, 515, 542, 572902, 1041, 1341, 1425, 1448, 1587, 1710, 1746, 1793, 27033091, 3663, 3777MwoI (23)gcnnnnnnngc15, 99, 143, 250, 409, 439, 471, 515, 542, 572902, 1041, 1341, 1425, 1448, 1587, 1710, 1746, 1793, 27033091, 3663, 3777AluI (22)agct58, 122, 217, 239, 254, 377, 591, 848, 969, 10221048, 1094, 1454, 1912, 2466, 2529, 2629, 3150, 3407, 34533543, 3769M.AluI (22)agct58, 122, 217, 239, 254, 377, 591, 848, 969, 10221048, 1094, 1454, 1912, 2466, 2529, 2629, 3150, 3407, 34533543, 3769M.EsaBC1I (22)agct58, 122, 217, 239, 254, 377, 591, 848, 969, 10221048, 1094, 1454, 1912, 2466, 2529, 2629, 3150, 3407, 34533543, 3769HpaII (21)ccgg163, 575, 956, 1083, 1254, 1331, 1353, 1381, 1512, 16021669, 1850, 2283, 2525, 2635, 2702, 2736, 3140, 3330, 33563503M.BsuFI (21)ccgg163, 575, 956, 1083, 1254, 1331, 1353, 1381, 1512, 16021669, 1850, 2283, 2525, 2635, 2702, 2736, 3140, 3330, 33563503M.CboI (21)ccgg163, 575, 956, 1083, 1254, 1331, 1353, 1381, 1512, 16021669, 1850, 2283, 2525, 2635, 2702, 2736, 3140, 3330, 33563503M.HapII (21)ccgg163, 575, 956, 1083, 1254, 1331, 1353, 1381, 1512, 16021669, 1850, 2283, 2525, 2635, 2702, 2736, 3140, 3330, 33563503M.HpaII (21)ccgg163, 575, 956, 1083, 1254, 1331, 1353, 1381, 1512, 16021669, 1850, 2283, 2525, 2635, 2702, 2736, 3140, 3330, 33563503M.MjaVI (21)ccgg163, 575, 956, 1083, 1254, 1331, 1353, 1381, 1512, 16021669, 1850, 2283, 2525, 2635, 2702, 2736, 3140, 3330, 33563503M.MspI (21)ccgg163, 575, 956, 1083, 1254, 1331, 1353, 1381, 1512, 16021669, 1850, 2283, 2525, 2635, 2702, 2736, 3140, 3330, 33563503BbvI (20)gcagc241, 902, 1310, 1494, 1587, 2400, 3094, 3300, 57, 253357, 430, 498, 1411, 1453, 1974, 2766, 3368, 3787, 3805Hpy188III (20)tcnnga77, 315, 984, 1184, 1195, 1368, 1684, 1693, 1776, 18321876, 1966, 1999, 2731, 2990, 3064, 3147, 3245, 3343, 3477M.BbvI (20)gcagc241, 902, 1310, 1494, 1587, 2400, 3094, 3300, 57, 253357, 430, 498, 1411, 1453, 1974, 2766, 3368, 3787, 3805M.BbvSI (20)gcwgc57, 241, 253, 357, 430, 498, 902, 1310, 1411, 14531494, 1587, 1974, 2400, 2766, 3094, 3300, 3368, 3787, 3805TseI (20)gcwgc57, 241, 253, 357, 430, 498, 902, 1310, 1411, 14531494, 1587, 1974, 2400, 2766, 3094, 3300, 3368, 3787, 3805BsrI (18)actgg932, 2346, 3294, 3307, 81, 293, 319, 764, 974, 10551139, 1289, 1490, 2171, 2610, 2653, 2771, 3177M.EsaDix4I (17)ttaa51, 110, 345, 469, 740, 838, 855, 866, 878, 22412606, 2645, 2880, 2933, 2947, 2952, 3004M.EsaDix5I (17)ttaa51, 110, 345, 469, 740, 838, 855, 866, 878, 22412606, 2645, 2880, 2933, 2947, 2952, 3004M.Hpy99VA (17)cctc395, 1207, 1271, 1407, 1764, 2454, 2660, 3538, 3821, 25642, 1105, 1940, 2790, 2871, 3271, 3595M.MnlIA (17)cctc395, 1207, 1271, 1407, 1764, 2454, 2660, 3538, 3821, 25642, 1105, 1940, 2790, 2871, 3271, 3595M.MnlIB (17)cctc395, 1207, 1271, 1407, 1764, 2454, 2660, 3538, 3821, 25642, 1105, 1940, 2790, 2871, 3271, 3595M.MseI (17)ttaa51, 110, 345, 469, 740, 838, 855, 866, 878, 22412606, 2645, 2880, 2933, 2947, 2952, 3004MnlI (17)cctc395, 1207, 1271, 1407, 1764, 2454, 2660, 3538, 3821, 25642, 1105, 1940, 2790, 2871, 3271, 3595MseI (17)ttaa51, 110, 345, 469, 740, 838, 855, 866, 878, 22412606, 2645, 2880, 2933, 2947, 2952, 3004HaeIII (16)ggcc41, 295, 397, 686, 828, 1256, 1430, 1821, 1848, 24282695, 2775, 3233, 3667, 3685, 3696M.AvaVII (16)ggcc41, 295, 397, 686, 828, 1256, 1430, 1821, 1848, 24282695, 2775, 3233, 3667, 3685, 3696M.BflBF4I (16)gcsgc462, 476, 1155, 1258, 1321, 1416, 1581, 1823, 1919, 19602076, 2305, 2427, 3511, 3666, 3784M.BspRI (16)ggcc41, 295, 397, 686, 828, 1256, 1430, 1821, 1848, 24282695, 2775, 3233, 3667, 3685, 3696M.BsuRI (16)ggcc41, 295, 397, 686, 828, 1256, 1430, 1821, 1848, 24282695, 2775, 3233, 3667, 3685, 3696M.CglASI (16)gcsgc462, 476, 1155, 1258, 1321, 1416, 1581, 1823, 1919, 19602076, 2305, 2427, 3511, 3666, 3784M.HaeIII (16)ggcc41, 295, 397, 686, 828, 1256, 1430, 1821, 1848, 24282695, 2775, 3233, 3667, 3685, 3696M.MthTI (16)ggcc41, 295, 397, 686, 828, 1256, 1430, 1821, 1848, 24282695, 2775, 3233, 3667, 3685, 3696M.NgoGII (16)ggcc41, 295, 397, 686, 828, 1256, 1430, 1821, 1848, 24282695, 2775, 3233, 3667, 3685, 3696M.NgoPII (16)ggcc41, 295, 397, 686, 828, 1256, 1430, 1821, 1848, 24282695, 2775, 3233, 3667, 3685, 3696M.Phi3TI (16)ggcc41, 295, 397, 686, 828, 1256, 1430, 1821, 1848, 24282695, 2775, 3233, 3667, 3685, 3696M.Rho11sI (16)ggcc41, 295, 397, 686, 828, 1256, 1430, 1821, 1848, 24282695, 2775, 3233, 3667, 3685, 3696M.SPBetaI (16)ggcc41, 295, 397, 686, 828, 1256, 1430, 1821, 1848, 24282695, 2775, 3233, 3667, 3685, 3696M.SPRI (16)ggcc41, 295, 397, 686, 828, 1256, 1430, 1821, 1848, 24282695, 2775, 3233, 3667, 3685, 3696TauI (16)gcsgc462, 476, 1155, 1258, 1321, 1416, 1581, 1823, 1919, 19602076, 2305, 2427, 3511, 3666, 3784HpyCH4V (15)tgca55, 355, 1026, 1128, 1240, 1399, 1574, 1588, 2149, 23932481, 2674, 2764, 3099, 3395M.CviRI (15)tgca55, 355, 1026, 1128, 1240, 1399, 1574, 1588, 2149, 23932481, 2674, 2764, 3099, 3395M.CviSI (15)tgca55, 355, 1026, 1128, 1240, 1399, 1574, 1588, 2149, 23932481, 2674, 2764, 3099, 3395AlwI (14)ggatc1600, 2192, 2972, 3069, 1163, 1180, 1210, 1521, 1767, 21742495, 2959, 3057, 3143Hpy188I (14)tcnga621, 1166, 1189, 1315, 1659, 2012, 2321, 2441, 2452, 28983033, 3167, 3520, 3598M.AlwI (14)ggatc1600, 2192, 2972, 3069, 1163, 1180, 1210, 1521, 1767, 21742495, 2959, 3057, 3143M.Hpy188I (14)tcnga621, 1166, 1189, 1315, 1659, 2012, 2321, 2441, 2452, 28983033, 3167, 3520, 3598MaeIII (14)gtnac316, 336, 491, 503, 1471, 1777, 2161, 2349, 2502, 25602891, 3174, 3290, 3353CviAII (13)catg226, 1039, 1220, 1565, 1751, 1782, 1808, 2374, 2410, 24882498, 2989, 3709FatI (13)catg226, 1039, 1220, 1565, 1751, 1782, 1808, 2374, 2410, 24882498, 2989, 3709HpyCH4I (13)catg226, 1039, 1220, 1565, 1751, 1782, 1808, 2374, 2410, 24882498, 2989, 3709M.CviAII (13)catg226, 1039, 1220, 1565, 1751, 1782, 1808, 2374, 2410, 24882498, 2989, 3709M.CviQVII (13)catg226, 1039, 1220, 1565, 1751, 1782, 1808, 2374, 2410, 24882498, 2989, 3709M.CviSII (13)catg226, 1039, 1220, 1565, 1751, 1782, 1808, 2374, 2410, 24882498, 2989, 3709M.EsaBS1I (13)catg226, 1039, 1220, 1565, 1751, 1782, 1808, 2374, 2410, 24882498, 2989, 3709M.Hpy166IV (13)catg226, 1039, 1220, 1565, 1751, 1782, 1808, 2374, 2410, 24882498, 2989, 3709M.Hpy188II (13)catg226, 1039, 1220, 1565, 1751, 1782, 1808, 2374, 2410, 24882498, 2989, 3709M.Hpy99X (13)catg226, 1039, 1220, 1565, 1751, 1782, 1808, 2374, 2410, 24882498, 2989, 3709M.HpyAI (13)catg226, 1039, 1220, 1565, 1751, 1782, 1808, 2374, 2410, 24882498, 2989, 3709M.HpyI (13)catg226, 1039, 1220, 1565, 1751, 1782, 1808, 2374, 2410, 24882498, 2989, 3709M.NgoMV (13)ggnncc137, 607, 619, 640, 1102, 1350, 1385, 2523, 2734, 27752869, 3641, 3680M.NlaIII (13)catg226, 1039, 1220, 1565, 1751, 1782, 1808, 2374, 2410, 24882498, 2989, 3709M.Sse9I (13)aatt108, 183, 200, 260, 287, 863, 874, 885, 1087, 20152388, 2643, 2949NlaIII (13)catg226, 1039, 1220, 1565, 1751, 1782, 1808, 2374, 2410, 24882498, 2989, 3709NlaIV (13)ggnncc137, 607, 619, 640, 1102, 1350, 1385, 2523, 2734, 27752869, 3641, 3680Tsp509I (13)aatt108, 183, 200, 260, 287, 863, 874, 885, 1087, 20152388, 2643, 2949BstUI (12)cgcg33, 450, 474, 494, 870, 1414, 1715, 1747, 2260, 27533083, 3664M.BepI (12)cgcg33, 450, 474, 494, 870, 1414, 1715, 1747, 2260, 27533083, 3664M.BsuEII (12)cgcg33, 450, 474, 494, 870, 1414, 1715, 1747, 2260, 27533083, 3664M.DsaV (12)ccngg142, 330, 956, 1353, 1513, 1736, 2284, 2635, 3331, 35493562, 3683M.Ecl18kI (12)ccngg142, 330, 956, 1353, 1513, 1736, 2284, 2635, 3331, 35493562, 3683M.FnuDII (12)cgcg33, 450, 474, 494, 870, 1414, 1715, 1747, 2260, 27533083, 3664M.NlaX (12)ccngg142, 330, 956, 1353, 1513, 1736, 2284, 2635, 3331, 35493562, 3683M.SenPI (12)ccngg142, 330, 956, 1353, 1513, 1736, 2284, 2635, 3331, 35493562, 3683M.SsoII (12)ccngg142, 330, 956, 1353, 1513, 1736, 2284, 2635, 3331, 35493562, 3683M.TspRI (12)castg94, 292, 1029, 1473, 2396, 2423, 2770, 2875, 3024, 32953308, 3814ScrFI (12)ccngg142, 330, 956, 1353, 1513, 1736, 2284, 2635, 3331, 35493562, 3683SelI (12)cgcg33, 450, 474, 494, 870, 1414, 1715, 1747, 2260, 27533083, 3664StyD4I (12)ccngg142, 330, 956, 1353, 1513, 1736, 2284, 2635, 3331, 35493562, 3683TspRI (12)castg94, 292, 1029, 1473, 2396, 2423, 2770, 2875, 3024, 32953308, 3814HpyCH4III (11)acngt422, 704, 945, 1728, 1844, 1952, 2379, 2894, 3207, 36773748HpyCH4IV (11)acgt310, 568, 678, 721, 733, 921, 1465, 1652, 2218, 25913007M.Hpy99XI (11)acgt310, 568, 678, 721, 733, 921, 1465, 1652, 2218, 25913007M.HpyAIIA (11)gaaga549, 2009, 3060, 3212, 393, 1699, 1909, 2027, 2136, 22142969M.HpyCH4IV (11)acgt310, 568, 678, 721, 733, 921, 1465, 1652, 2218, 25913007M.MboIIA (11)gaaga549, 2009, 3060, 3212, 393, 1699, 1909, 2027, 2136, 22142969MboII (11)gaaga549, 2009, 3060, 3212, 393, 1699, 1909, 2027, 2136, 22142969TaiI (11)acgt310, 568, 678, 721, 733, 921, 1465, 1652, 2218, 25913007BccI (10)ccatc671, 1143, 1169, 1234, 1662, 2370, 2657, 2781, 689, 1562BscAI (10)gcatc1318, 1573, 1789, 2130, 2570, 3622, 1640, 1704, 1979, 2360BstF5I (10)ggatg363, 1532, 959, 1142, 1204, 1661, 1686, 2369, 2656, 2837BstYI (10)rgatcy1164, 1187, 1522, 1768, 2175, 2192, 2960, 2972, 3058, 3069FokI (10)ggatg363, 1532, 959, 1142, 1204, 1661, 1686, 2369, 2656, 2837HinfI (10)gantc46, 276, 728, 750, 1835, 1969, 2822, 3339, 3735, 3810HpyAV (10)ccttc1484, 2462, 3170, 3480, 414, 554, 1985, 1995, 2087, 2699M.BabI (10)gantc46, 276, 728, 750, 1835, 1969, 2822, 3339, 3735, 3810M.BspST5I (10)gcatc1318, 1573, 1789, 2130, 2570, 3622, 1640, 1704, 1979, 2360M.BstF5IA (10)ggatg363, 1532, 959, 1142, 1204, 1661, 1686, 2369, 2656, 2837M.BstF5ID (10)ggatg363, 1532, 959, 1142, 1204, 1661, 1686, 2369, 2656, 2837M.BstYI (10)rgatcy1164, 1187, 1522, 1768, 2175, 2192, 2960, 2972, 3058, 3069M.CcrMI (10)gantc46, 276, 728, 750, 1835, 1969, 2822, 3339, 3735, 3810M.CviBI (10)gantc46, 276, 728, 750, 1835, 1969, 2822, 3339, 3735, 3810M.CviQVI (10)gantc46, 276, 728, 750, 1835, 1969, 2822, 3339, 3735, 3810M.FokI (10)ggatg363, 1532, 959, 1142, 1204, 1661, 1686, 2369, 2656, 2837M.HhaII (10)gantc46, 276, 728, 750, 1835, 1969, 2822, 3339, 3735, 3810M.HinfI (10)gantc46, 276, 728, 750, 1835, 1969, 2822, 3339, 3735, 3810M.Hpy99IX (10)gantc46, 276, 728, 750, 1835, 1969, 2822, 3339, 3735, 3810M.HpyAIV (10)gantc46, 276, 728, 750, 1835, 1969, 2822, 3339, 3735, 3810M.SscL1I (10)gantc46, 276, 728, 750, 1835, 1969, 2822, 3339, 3735, 3810M.StsI (10)ggatg363, 1532, 959, 1142, 1204, 1661, 1686, 2369, 2656, 2837M.XhoII (10)rgatcy1164, 1187, 1522, 1768, 2175, 2192, 2960, 2972, 3058, 3069SfaNI (10)gcatc1318, 1573, 1789, 2130, 2570, 3622, 1640, 1704, 1979, 2360StsI (10)ggatg363, 1532, 959, 1142, 1204, 1661, 1686, 2369, 2656, 2837BslI (9)ccnnnnnnngg39, 461, 787, 1331, 1875, 3231, 3510, 3676, 3694Bsp1286I (9)gdgchc606, 1293, 1386, 1460, 1650, 1712, 2150, 2235, 3396Hpy99I (9)cgwcg299, 312, 1436, 1464, 1758, 1773, 2552, 2815, 3609EsaBC3I (8)tcga644, 1462, 1618, 1642, 1678, 1840, 2166, 3610M.CviBIII (8)tcga644, 1462, 1618, 1642, 1678, 1840, 2166, 3610M.CviQIII (8)tcga644, 1462, 1618, 1642, 1678, 1840, 2166, 3610M.CviSIII (8)tcga644, 1462, 1618, 1642, 1678, 1840, 2166, 3610M.EsaBC3I (8)tcga644, 1462, 1618, 1642, 1678, 1840, 2166, 3610M.EsaDix3I (8)tcga644, 1462, 1618, 1642, 1678, 1840, 2166, 3610M.HpyAX (8)tcga644, 1462, 1618, 1642, 1678, 1840, 2166, 3610M.Phi3TII (8)tcga644, 1462, 1618, 1642, 1678, 1840, 2166, 3610M.TaqI (8)tcga644, 1462, 1618, 1642, 1678, 1840, 2166, 3610M.TthHB8I (8)tcga644, 1462, 1618, 1642, 1678, 1840, 2166, 3610TaqI (8)tcga644, 1462, 1618, 1642, 1678, 1840, 2166, 3610BsaJI (7)ccnngg142, 330, 956, 1160, 1514, 1783, 3550CdiI (7)catcg1788, 2569, 690, 1636, 1839, 1980, 2165EaeI (7)yggccr42, 296, 1257, 1431, 1822, 1849, 2429FmuI (7)ggncc398, 686, 1866, 2457, 2679, 2696, 2775GdiII (7)cggccr42, 296, 1257, 1822, 1849, 1257, 2429Hpy8I (7)gtnnac676, 731, 748, 1136, 2150, 2908, 3396M.Cfr13I (7)ggncc398, 686, 1866, 2457, 2679, 2696, 2775M.CfrI (7)yggccr42, 296, 1257, 1431, 1822, 1849, 2429M.EaeI (7)yggccr42, 296, 1257, 1431, 1822, 1849, 2429M.EcoHK31I (7)yggccr42, 296, 1257, 1431, 1822, 1849, 2429M.Hpy8I (7)gtnnac676, 731, 748, 1136, 2150, 2908, 3396M.HpyAIX (7)gtnnac676, 731, 748, 1136, 2150, 2908, 3396M.PspPI (7)ggncc398, 686, 1866, 2457, 2679, 2696, 2775M.Sau96I (7)ggncc398, 686, 1866, 2457, 2679, 2696, 2775MspA1I (7)cmgckg59, 378, 1095, 1455, 2184, 3125, 3370Sau96I (7)ggncc398, 686, 1866, 2457, 2679, 2696, 2775TspDTI (7)atgaa48, 1837, 2941, 961, 1394, 2536, 2839UnbI (7)ggncc398, 686, 1866, 2457, 2679, 2696, 2775BanI (6)ggyrcc137, 640, 1102, 1350, 1385, 2869BsiEI (6)cgrycg408, 1257, 2301, 2450, 3373, 3797BstNI (6)ccwgg142, 330, 1736, 3549, 3562, 3683CjeI (6)ccannnnnngt3128, 3683, 382, 3029, 3129, 3207EcoHI (6)ccsgg956, 1353, 1513, 2284, 2635, 3331FauI (6)cccgc89, 479, 1480, 32, 400, 533HaeII (6)rgcgcy7, 522, 530, 1102, 1350, 3466Hin4I (6)gaynnnnnvtc1528, 1558, 2748, 2822, 1528, 2822HphI (6)ggtga2102, 2343, 668, 2117, 2739, 2966LpnI (6)rgcgcy7, 522, 530, 1102, 1350, 3466M.BcnIA (6)ccsgg956, 1353, 1513, 2284, 2635, 3331M.BcnIB (6)ccsgg956, 1353, 1513, 2284, 2635, 3331M.BstNI (6)ccwgg142, 330, 1736, 3549, 3562, 3683M.Eco1831I (6)ccsgg956, 1353, 1513, 2284, 2635, 3331M.EcoHI (6)ccsgg956, 1353, 1513, 2284, 2635, 3331M.EcoKDcm (6)ccwgg142, 330, 1736, 3549, 3562, 3683M.EcoPI (6)agacc790, 1672, 2749, 1370, 2900, 3035M.EcoRII (6)ccwgg142, 330, 1736, 3549, 3562, 3683M.HgiCI (6)ggyrcc137, 640, 1102, 1350, 1385, 2869M.HphIA (6)ggtga2102, 2343, 668, 2117, 2739, 2966M.HphIB (6)ggtga2102, 2343, 668, 2117, 2739, 2966M.Hpy99II (6)gtsac316, 503, 1471, 1777, 2349, 2560M.MlyI (6)gastc276, 728, 750, 2822, 3339, 3810M.MvaI (6)ccwgg142, 330, 1736, 3549, 3562, 3683M.NgoBVIIIA (6)ggtga2102, 2343, 668, 2117, 2739, 2966M.NgoHVIII (6)ggtga2102, 2343, 668, 2117, 2739, 2966M.StyLTI (6)cagag1107, 1314, 3251, 3277, 3597, 3188M.Tsp45I (6)gtsac316, 503, 1471, 1777, 2349, 2560MlyI (6)gagtc750, 3339, 276, 728, 2822, 3810NciI (6)ccsgg956, 1353, 1513, 2284, 2635, 3331PleI (6)gagtc750, 3339, 276, 728, 2822, 3810PspGI (6)ccwgg142, 330, 1736, 3549, 3562, 3683SfcI (6)ctryag270, 458, 1400, 2576, 3254, 3445SimI (6)gggtc647, 953, 1779, 3036, 2748, 3517SspD5I (6)ggtga2102, 2343, 668, 2117, 2739, 2966Tsp45I (6)gtsac316, 503, 1471, 1777, 2349, 2560BceAI (5)acggc297, 1324, 3224, 637, 1760BcefI (5)acggc297, 1324, 3224, 637, 1760BfaI (5)ctag526, 1050, 2627, 2962, 3215BmrI (5)actggg320, 975, 1056, 1290, 2772BpuEI (5)cttgag1742, 3342, 2197, 3065, 3604BsaWI (5)wccggw1084, 1382, 2526, 3357, 3504BsiHKAI (5)gwgcwc1460, 1650, 2150, 2235, 3396DdeI (5)ctnag247, 2320, 2860, 3026, 3435Eco57MI (5)ctgrag3183, 1476, 1908, 2135, 2732M.BfiIA (5)actggg320, 975, 1056, 1290, 2772M.BfiIB (5)actggg320, 975, 1056, 1290, 2772M.DdeI (5)ctnag247, 2320, 2860, 3026, 3435M.MthZI (5)ctag526, 1050, 2627, 2962, 3215SmlI (5)ctyrag1742, 2197, 3065, 3342, 3604TaqII (5)gaccga789, 2300, 1372, 2459, 3798AcuI (4)ctgaag3183, 1476, 1908, 2135Bme1580I (4)gkgcmc1293, 1386, 2150, 3396BseMII (4)ctcag2860, 3026, 3435, 2320BspCNI (4)ctcag2860, 3026, 3435, 2320BsrBI (4)ccgctc191, 535, 1962, 3779BsrFI (4)rccggy576, 1670, 1851, 2737BtsI (4)gcagtg2424, 94, 1029, 2396CjePI (4)ccannnnnnntc3177, 2438, 2910, 3030EarI (4)ctcttc394, 1700, 1910, 2028EciI (4)ggcgga1864, 2664, 3492, 3638HaeI (4)wggccw1431, 3234, 3686, 3697HgaI (4)gacgc3030, 3608, 449, 2280M.AvaIX (4)rccggy576, 1670, 1851, 2737M.Cfr10I (4)rccggy576, 1670, 1851, 2737M.Cfr6I (4)cagctg59, 378, 1095, 1455M.Eco57IA (4)ctgaag3183, 1476, 1908, 2135M.Eco57IB (4)ctgaag3183, 1476, 1908, 2135M.HgaIA (4)gacgc3030, 3608, 449, 2280M.HgaIB (4)gacgc3030, 3608, 449, 2280M.PvuII (4)cagctg59, 378, 1095, 1455M.TfiI (4)gawtc46, 1835, 1969, 3735MslI (4)caynnnnrtg1788, 2051, 2410, 2569PvuII (4)cagctg59, 378, 1095, 1455TfiI (4)gawtc46, 1835, 1969, 3735Tth111II (4)caarca1796, 3097, 3136, 3103AceIII (3)cagctc849, 2530, 3770ApoI (3)raatty864, 875, 886AseI (3)attaat52, 111, 2646AvaII (3)ggwcc1866, 2457, 2679BglI (3)gccnnnnnggc250, 439, 2703BsaAI (3)yacgtr679, 922, 1653BsaHI (3)grcgyc1102, 1350, 2281BseYI (3)cccagc1097, 1855, 3406BspMI (3)acctgc1018, 1247, 1610BsrDI (3)gcaatg2763, 1577, 2581BssSI (3)cacgag1943, 2153, 3537DraI (3)tttaaa2242, 2934, 2953DrdI (3)gacnnnnnngtc728, 1378, 3608FspI (3)tgcgca428, 1451, 2597M.BsaAI (3)yacgtr679, 922, 1653M.DraI (3)tttaaa2242, 2934, 2953M.EcoP15I (3)cagcag3093, 3302, 3186M.MmeIA (3)tccrac725, 3336, 3520M.MmeIB (3)tccrac725, 3336, 3520M.SinI (3)ggwcc1866, 2457, 2679M.VspI (3)attaat52, 111, 2646MmeI (3)tccrac725, 3336, 3520PpiI (3)gaacnnnnnctc735, 3019, 2176Psp03I (3)ggwcc1866, 2457, 2679VpaK11AI (3)ggwcc1866, 2457, 2679AclI (2)aacgtt2219, 2592AflIII (2)acrygt921, 3710AlwNI (2)cagnnnctg973, 3299ApaLI (2)gtgcac2150, 3396BanII (2)grgcyc606, 1712BbeI (2)ggcgcc1102, 1350BciVI (2)gtatcc1559, 3501BsaBI (2)gatnnnnatc1186, 1210BsaXI (2)acnnnnnctcc733, 28BsmAI (2)gtctc1198, 2750Csp6I (2)gtac1654, 2338FalI (2)aagnnnnnctt245, 1673HaeIV (2)gaynnnnnrtc1528, 2822KasI (2)ggcgcc1102, 1350M.AclI (2)aacgtt2219, 2592M.AflIII (2)acrygt921, 3710M.Alw26I (2)gtctc1198, 2750M.BanII (2)grgcyc606, 1712M.CfrBI (2)ccwwgg1160, 1783M.CviQI (2)gtac1654, 2338M.CviRII (2)gtac1654, 2338M.HaeIV (2)gaynnnnnrtc1528, 2822M.NgoBIX (2)gtannnnnctc2161, 3443M.NgoMIV (2)gccggc576, 1851M.NspHI (2)rcatgy1752, 3710M.NspI (2)rcatgy1752, 3710M.PhiMx8I (2)ctssag1400, 2732M.RsaI (2)gtac1654, 2338M.SauLPI (2)gccggc576, 1851NaeI (2)gccggc576, 1851NarI (2)ggcgcc1102, 1350NgoMIV (2)gccggc576, 1851NspI (2)rcatgy1752, 3710PvuI (2)cgatcg408, 2450RsaI (2)gtac1654, 2338SapI (2)gctcttc1701, 1911SfoI (2)ggcgcc1102, 1350StyI (2)ccwwgg1160, 1783TspGWI (2)acgga2050, 2367AhdI (1)gacnnnnngtc2822AloI (1)gaacnnnnnntcc735ApaBI (1)gcannnnntgc902BcgI (1)cgannnnnntgc2301BclI (1)tgatca1192BglII (1)agatct1187BpmI (1)ctggag2732BsaI (1)ggtctc2750BsmFI (1)gggac1487Bsp24I (1)gacnnnnnntgg3030BspHI (1)tcatga2990BssHII (1)gcgcgc1748BstAPI (1)gcannnnntgc902Bsu36I (1)cctnagg248BtgI (1)ccrygg1783DraIII (1)cacnnngtg682EagI (1)cggccg1257M.AloI (1)gaacnnnnnntcc735M.BcgI (1)cgannnnnntgc2301M.BclI (1)tgatca1192M.BglII (1)agatct1187M.BmeTI (1)tgatca1192M.BspLU11IIIA (1)gggac1487M.BspLU11IIIC (1)gggac1487M.BsuBI (1)ctgcag1400M.DraIII (1)cacnnngtg682M.EcoKI (1)aacnnnnnngtgc2159M.EcoR124II (1)gaannnnnnnrtcg1641M.NmeSI (1)agtact2339M.PstI (1)ctgcag1400M.ScaI (1)agtact2339M.StyLTIII (1)gagnnnnnnrtayg2318M.StySPI (1)aacnnnnnngtrc2159M.TaeII (1)tgatca1192M.Tth111I (1)gacnnngtc1469M.XcmI (1)ccannnnnnnnntgg1169M.XmaIII (1)cggccg1257MscI (1)tggcca1431PciI (1)acatgt3710PsiI (1)ttataa807PstI (1)ctgcag1400RsrII (1)cggwccg1867ScaI (1)agtact2339SphI (1)gcatgc1752TatI (1)wgtacw2339Tth111I (1)gacnnngtc1469XcmI (1)ccannnnnnnnntgg1169XmnI (1)gaannnnttc2222 Non-Cutters AarI, AatII, Acc65I, AccI, AfeI, AflII, AgeI, AleI, ApaI, AscI AsiSI, AvaI, AvrII, BaeI, BamHI, Bbr7I, BbsI, BbvCI, BfrBI, BlpI BmgBI, BmtI, BplI, Bpu10I, BseRI, BsgI, BsiWI, BsmBI, BsmI, BspEI BsrGI, BstBI, BstEII, BstXI, BstZ17I, ClaI, EcoICRI, EcoNI, EcoO109I, EcoRI EcoRV, FseI, FspAI, HincII, HindIII, HpaI, KpnI, M.AccI, M.ApaI, M.AquI M.BaeI, M.BbvCIA, M.BbvCIB, M.BnaI, M.BseCI, M.Bsp106I, M.BstI, M.BstVI, M.Bsu15I, M.Cfr9I M.ClaI, M.EcaI, M.EcoAI, M.EcoBI, M.EcoO109I, M.EcoR124I, M.EcoRI, M.EcoRV, M.Esp3I, M.HincII M.HindII, M.HindIII, M.HpaI, M.KpnAI, M.KpnBI, M.KpnI, M.MunI, M.NgoAV, M.PaeR7I, M.PhiBssHII "    ) A m r 2:TVe2hh6CJKHOJQJ]^JaJmH sH $hk0J5CJOJQJ^JaJo(!hk0J5CJOJQJ^JaJ hgso(hphgso( h0kmo( hhgs hqhgs *hgsB*o(phhPhgsB*o(phhPhgsB*ph hKhgs hphgs hgs5 hgs5o(!"L*z.Yuvwxyz{|}~ $7$8$H$a$gd$a$gdk(*hjxz$-OXYjtuĬĬăsbQ阃EhhgsmH o(sH !hCJKHOJQJ^JaJo(!hnhCJKHOJQJ^JaJo(hCJKHOJQJ^JaJ)hnhCJKHOJQJ^JaJmH o(sH &hnhCJKHOJQJ^JaJmH sH 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la<ۖzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdH$$IflF@  ppp0    4 la<ۖܖzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdnI$$IflF@  ppp0    4 la<$Fzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdJ$$IflF@  ppp0    4 la<FGPVrzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdJ$$IflF@  ppp0    4 la<rs}zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd-K$$IflF@  ppp0    4 la<ܗzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdK$$IflF@  ppp0    4 la<ܗݗzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdWL$$IflF@  ppp0    4 la<=zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdL$$IflF@  ppp0    4 la<=>JPrzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdM$$IflF@  ppp0    4 la<rszk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdN$$IflF@  ppp0    4 la<ۘzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdN$$IflF@  ppp0    4 la<ۘܘzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd@O$$IflF@  ppp0    4 la< &Hzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdO$$IflF@  ppp0    4 la<HIW]~zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdjP$$IflF@  ppp0    4 la<~zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdP$$IflF@  ppp0    4 la<Ǚzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdQ$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd)R$$IflF@ 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la<@ALSczk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kds$$IflF@  ppp0    4 la<cdmtzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdt$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdu$$IflF@  ppp0    4 la<äzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdu$$IflF@  ppp0    4 la<äĤͤ٤zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd?v$$IflF@  ppp0    4 la< zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdv$$IflF@  ppp0    4 la< ,zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdiw$$IflF@  ppp0    4 la<,-7>Ozk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdw$$IflF@  ppp0    4 la<OPZarzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdx$$IflF@  ppp0    4 la<rs}zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd(y$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdy$$IflF@  ppp0    4 la<¥ɥڥzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdRz$$IflF@  ppp0    4 la<ڥۥzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdz$$IflF@  ppp0    4 la< "zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd|{$$IflF@  ppp0    4 la<"#/6Ezk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd|$$IflF@  ppp0    4 la<EFQXizk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd|$$IflF@  ppp0    4 la<ijxzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd;}$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd}$$IflF@  ppp0    4 la<Ȧئzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kde~$$IflF@  ppp0    4 la<ئ٦zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd~$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<&-=zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$$IflF@  ppp0    4 la<=>GTdzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<depvzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdN$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<ȧzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdx$$IflF@  ppp0    4 la<ȧɧԧۧzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd $$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<!zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd7$$IflF@  ppp0    4 la<!",3=zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd̄$$IflF@  ppp0    4 la<=>GNYzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kda$$IflF@  ppp0    4 la<YZdkvzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<vwzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  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la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdp$$IflF@  ppp0    4 la<Īzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<ĪŪӪڪzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd/$$IflF@  ppp0    4 la< zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdĒ$$IflF@  ppp0    4 la< !*1;zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdY$$IflF@  ppp0    4 la<;<ELWzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<WXcjtzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<tu~zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<ūzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdB$$IflF@  ppp0    4 la<ūƫϫ׫zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdז$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdl$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<&,7zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<78AMRzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd+$$IflF@  ppp0    4 la<RS\jnzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    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la<#*/zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd:$$IflF@  ppp0    4 la</0<CHzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdϤ$$IflF@  ppp0    4 la<HIU\azk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdd$$IflF@  ppp0    4 la<abtzzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd#$$IflF@  ppp0    4 la<ĮήҮzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<ҮӮ߮zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdM$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<$+0zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdw$$IflF@  ppp0    4 la<01<CHzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd $$IflF@  ppp0    4 la<HIT[`zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<`apzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd6$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd˫$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd`$$IflF@  ppp0    4 la<ͯׯܯzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<ܯݯzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0 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