ࡱ>  xzopqrstuvwg RbjbjVV2r<r< HH8+W+++++,,,VVVVVVV$Xi[PV9-,,--V++4V6V6V6V-&++V6V-V6V6V6V+oBT6VrVV0+W6V\PU\6V\6V<,-6V- -,,,VVV,,,+W----\,,,,,,,,,H, t: pEASY-Blunt Simple Cloning Vector Complete sequence AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTGCCCTTAAGGGCAGCTTCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCCCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG M13F5'-GTAAAACGACGGCCAGT-3' vU_SGJ101-01 M13R5'-CAGGAAACAGCTATGAC-3' vU_SGJ101-11 LacZa fragment: bases 1-449 M13 reverse priming site: bases 205-221 T7 promoter/priming site: bases 265-282 M13 Forward (-20) priming site: bases 290-305 f1 origin: bases 447-884 Kanamycin resistance ORF: bases 1218-2012 Ampicillin resistance ORF: bases 2030-2890 pUC origin: bases 3035-3708 pEASY-Blunt Simple Cloning Vector Restriction Map NEWMOL Cutters M.CviQII (349)rar25, 46, 72, 123, 127, 145, 152, 159, 166, 201245, 256, 262, 289, 303, 344, 353, 369, 414, 514536, 540, 545, 549, 554, 558, 562, 571, 579, 593607, 611, 619, 625, 632, 643, 658, 675, 707, 716736, 751, 757, 775, 786, 795, 799, 804, 815, 824854, 865, 876, 887, 1024, 1035, 1063, 1106, 1147, 11511215, 1250, 1261, 1278, 1313, 1329, 1357, 1361, 1444, 14611524, 1539, 1543, 1596, 1617, 1657, 1674, 1713, 1731, 17401770, 1796, 1826, 1836, 1914, 1937, 1941, 1947, 1963, 19701985, 1995, 2007, 2040, 2047, 2060, 2064, 2068, 2072, 20812087, 2094, 2100, 2177, 2195, 2203, 2221, 2238, 2247, 22972317, 2329, 2341, 2363, 2434, 2438, 2475, 2479, 2505, 25102621, 2625, 2631, 2684, 2692, 2699, 2714, 2751, 2791, 28722907, 2911, 2921, 2932, 2939, 2944, 2951, 2975, 2987, 30033014, 3020, 3060, 3072, 3076, 3097, 3132, 3136, 3159, 31633181, 3210, 3241, 3250, 3272, 3276, 3326, 3340, 3391, 34093473, 3545, 3569, 3587, 3596, 3602, 3614, 3625, 3670, 36803688, 3699, 3705, 3713, 3717, 3736, 3760, 3780, 18, 8396, 116, 181, 188, 211, 237, 248, 275, 323, 392436, 443, 471, 590, 597, 652, 727, 761, 767, 809840, 847, 860, 873, 883, 897, 907, 911, 917, 926941, 962, 989, 993, 1001, 1007, 1012, 1076, 1080, 10861113, 1121, 1131, 1136, 1160, 1184, 1195, 1207, 1227, 12311269, 1368, 1391, 1395, 1407, 1476, 1483, 1506, 1551, 15551627, 1631, 1644, 1648, 1665, 1698, 1725, 1743, 1764, 18021815, 1908, 1923, 2004, 2014, 2019, 2023, 2027, 2035, 21082119, 2125, 2135, 2154, 2168, 2189, 2198, 2213, 2233, 22432289, 2323, 2336, 2354, 2383, 2387, 2413, 2454, 2461, 24652522, 2528, 2533, 2537, 2548, 2555, 2600, 2615, 2660, 26692726, 2733, 2741, 2786, 2821, 2840, 2844, 2861, 2882, 29042935, 2954, 2968, 2996, 3011, 3027, 3044, 3053, 3066, 31023106, 3110, 3147, 3169, 3189, 3202, 3245, 3317, 3343, 33623381, 3413, 3417, 3431, 3436, 3452, 3459, 3463, 3479, 34843488, 3508, 3522, 3528, 3538, 3542, 3555, 3605, 3640, 36493764, 3768, 3792, 3800, 3809, 3817, 3821, 3825, 3830M.CviPI (299)gc4, 15, 22, 32, 40, 54, 57, 61, 86, 9097, 104, 121, 134, 143, 157, 164, 189, 216, 233238, 241, 251, 254, 264, 295, 332, 350, 355, 358373, 377, 381, 389, 397, 401, 410, 426, 431, 440450, 460, 463, 472, 477, 494, 499, 507, 516, 520528, 534, 543, 564, 573, 577, 591, 605, 630, 638686, 692, 712, 818, 828, 848, 870, 892, 900, 903913, 929, 937, 948, 969, 977, 998, 1002, 1015, 10221026, 1033, 1042, 1048, 1058, 1073, 1077, 1090, 1094, 11001123, 1128, 1145, 1153, 1156, 1171, 1218, 1240, 1244, 12561259, 1273, 1281, 1292, 1308, 1311, 1319, 1322, 1333, 13401348, 1385, 1399, 1409, 1412, 1417, 1426, 1430, 1440, 14491454, 1459, 1478, 1492, 1495, 1503, 1510, 1516, 1541, 15471568, 1574, 1579, 1582, 1585, 1588, 1594, 1604, 1611, 16281638, 1645, 1649, 1667, 1702, 1711, 1715, 1721, 1733, 17381745, 1749, 1753, 1761, 1785, 1790, 1794, 1798, 1821, 18241848, 1852, 1862, 1869, 1883, 1889, 1904, 1912, 1917, 19201928, 1935, 1945, 1958, 1961, 1972, 1975, 1982, 1992, 20562074, 2077, 2084, 2098, 2112, 2131, 2149, 2183, 2207, 22342250, 2258, 2281, 2286, 2292, 2303, 2306, 2358, 2372, 23932398, 2401, 2425, 2428, 2466, 2473, 2481, 2507, 2529, 25392556, 2571, 2578, 2584, 2595, 2612, 2629, 2637, 2662, 26742688, 2695, 2704, 2708, 2719, 2734, 2742, 2753, 2764, 27672775, 2787, 2827, 2857, 2869, 2883, 3028, 3081, 3092, 30953099, 3117, 3124, 3138, 3150, 3179, 3185, 3190, 3222, 32333256, 3261, 3274, 3279, 3298, 3301, 3304, 3311, 3364, 33693377, 3395, 3402, 3407, 3414, 3447, 3453, 3464, 3471, 34903509, 3512, 3531, 3543, 3559, 3590, 3606, 3623, 3636, 36413655, 3659, 3664, 3667, 3685, 3692, 3696, 3703, 3721, 37573769, 3778, 3782, 3785, 3788, 3801, 3806, 3818, 3826M.SssI (256)cg5, 14, 21, 31, 35, 42, 64, 76, 87, 98103, 162, 167, 190, 232, 263, 278, 297, 300, 310313, 333, 349, 372, 382, 390, 400, 405, 409, 427441, 449, 461, 464, 473, 478, 493, 500, 506, 521529, 533, 542, 563, 568, 572, 575, 585, 600, 621636, 644, 678, 691, 703, 711, 721, 733, 787, 820826, 869, 893, 914, 921, 936, 944, 956, 997, 10031043, 1059, 1078, 1083, 1101, 1155, 1172, 1212, 1217, 12431254, 1258, 1279, 1306, 1321, 1324, 1331, 1341, 1349, 13531372, 1381, 1405, 1413, 1422, 1434, 1437, 1441, 1450, 14621465, 1479, 1504, 1512, 1549, 1580, 1583, 1593, 1602, 16181629, 1637, 1642, 1646, 1652, 1658, 1669, 1678, 1696, 17141723, 1732, 1746, 1756, 1759, 1762, 1771, 1774, 1786, 18001823, 1840, 1850, 1863, 1868, 1884, 1895, 1918, 1921, 19341942, 1951, 1957, 1960, 1966, 1971, 1976, 1981, 1991, 20022050, 2055, 2075, 2111, 2152, 2166, 2184, 2206, 2211, 22182259, 2272, 2280, 2283, 2298, 2302, 2305, 2366, 2426, 24472451, 2459, 2472, 2506, 2515, 2525, 2550, 2553, 2557, 25672591, 2596, 2613, 2635, 2663, 2689, 2693, 2702, 2736, 27432752, 2794, 2799, 2813, 2816, 2842, 2856, 3007, 3017, 30293039, 3082, 3116, 3125, 3140, 3167, 3191, 3224, 3260, 32733312, 3320, 3330, 3346, 3356, 3365, 3370, 3374, 3383, 33923415, 3419, 3429, 3448, 3465, 3470, 3477, 3491, 3503, 35103519, 3532, 3536, 3558, 3582, 3589, 3607, 3610, 3626, 36373654, 3663, 3676, 3722, 3748, 3756, 3777, 3781, 3784, 37903794, 3798, 3802, 3807, 3819, 3827M.CviAIV (254)rgcb7, 23, 41, 58, 122, 144, 165, 217, 234, 239255, 265, 296, 351, 374, 398, 411, 432, 453, 508523, 531, 544, 565, 574, 592, 606, 687, 693, 713829, 849, 901, 930, 970, 978, 1023, 1034, 1049, 10951103, 1124, 1146, 1157, 1257, 1274, 1282, 1309, 1323, 13341351, 1418, 1427, 1455, 1493, 1569, 1586, 1595, 1605, 16681712, 1718, 1734, 1739, 1822, 1849, 1870, 1890, 1913, 19291936, 1959, 1983, 1993, 2057, 2113, 2208, 2282, 2304, 24292467, 2474, 2508, 2530, 2540, 2630, 2691, 2696, 2705, 27352776, 2788, 2858, 3118, 3151, 3180, 3223, 3234, 3262, 32753299, 3378, 3403, 3408, 3454, 3467, 3472, 3544, 3560, 35913642, 3656, 3668, 3686, 3697, 3758, 3770, 3779, 3789, 541, 58, 87, 98, 122, 144, 165, 190, 217, 239255, 296, 333, 378, 390, 398, 432, 441, 461, 473478, 500, 521, 529, 578, 592, 606, 687, 829, 849893, 901, 914, 930, 970, 978, 1003, 1023, 1034, 10431049, 1059, 1078, 1095, 1101, 1124, 1146, 1172, 1257, 12741282, 1309, 1334, 1341, 1349, 1413, 1418, 1427, 1441, 14551479, 1493, 1504, 1569, 1583, 1605, 1629, 1646, 1668, 17121722, 1739, 1746, 1762, 1786, 1822, 1849, 1863, 1884, 18901913, 1918, 1929, 1976, 2184, 2259, 2429, 2467, 2530, 25402557, 2613, 2630, 2663, 2696, 2705, 2735, 2743, 2776, 27883029, 3125, 3151, 3180, 3191, 3223, 3234, 3299, 3312, 33653370, 3378, 3403, 3408, 3415, 3448, 3454, 3465, 3491, 35103532, 3544, 3607, 3637, 3642, 3668, 3686, 3697, 3770, 37893802, 3807, 3819, 3827M.CviJI (254)rgcb7, 23, 41, 58, 122, 144, 165, 217, 234, 239255, 265, 296, 351, 374, 398, 411, 432, 453, 508523, 531, 544, 565, 574, 592, 606, 687, 693, 713829, 849, 901, 930, 970, 978, 1023, 1034, 1049, 10951103, 1124, 1146, 1157, 1257, 1274, 1282, 1309, 1323, 13341351, 1418, 1427, 1455, 1493, 1569, 1586, 1595, 1605, 16681712, 1718, 1734, 1739, 1822, 1849, 1870, 1890, 1913, 19291936, 1959, 1983, 1993, 2057, 2113, 2208, 2282, 2304, 24292467, 2474, 2508, 2530, 2540, 2630, 2691, 2696, 2705, 27352776, 2788, 2858, 3118, 3151, 3180, 3223, 3234, 3262, 32753299, 3378, 3403, 3408, 3454, 3467, 3472, 3544, 3560, 35913642, 3656, 3668, 3686, 3697, 3758, 3770, 3779, 3789, 541, 58, 87, 98, 122, 144, 165, 190, 217, 239255, 296, 333, 378, 390, 398, 432, 441, 461, 473478, 500, 521, 529, 578, 592, 606, 687, 829, 849893, 901, 914, 930, 970, 978, 1003, 1023, 1034, 10431049, 1059, 1078, 1095, 1101, 1124, 1146, 1172, 1257, 12741282, 1309, 1334, 1341, 1349, 1413, 1418, 1427, 1441, 14551479, 1493, 1504, 1569, 1583, 1605, 1629, 1646, 1668, 17121722, 1739, 1746, 1762, 1786, 1822, 1849, 1863, 1884, 18901913, 1918, 1929, 1976, 2184, 2259, 2429, 2467, 2530, 25402557, 2613, 2630, 2663, 2696, 2705, 2735, 2743, 2776, 27883029, 3125, 3151, 3180, 3191, 3223, 3234, 3299, 3312, 33653370, 3378, 3403, 3408, 3415, 3448, 3454, 3465, 3491, 35103532, 3544, 3607, 3637, 3642, 3668, 3686, 3697, 3770, 37893802, 3807, 3819, 3827M.HindI (96)cac94, 114, 176, 186, 273, 315, 482, 487, 502, 629668, 683, 746, 947, 1030, 1266, 1326, 1384, 1424, 14581509, 1776, 1812, 1846, 1858, 1897, 1944, 2052, 2117, 21482156, 2256, 2397, 2415, 2559, 2739, 2745, 2868, 2966, 30093128, 3218, 3296, 3309, 3322, 3394, 3450, 3619, 3740, 37663815, 63, 125, 136, 150, 204, 291, 360, 403, 512567, 640, 677, 732, 773, 920, 1242, 1294, 1433, 14721536, 1623, 1651, 2102, 2151, 2236, 2312, 2343, 2350, 24222483, 2563, 2682, 2769, 2812, 2874, 3023, 3114, 3236, 32583397, 3427, 3469, 3535, 3593, 3707CviJI (75)rgcy41, 58, 122, 144, 165, 217, 239, 255, 296, 378398, 432, 578, 592, 606, 687, 829, 849, 901, 930970, 978, 1023, 1034, 1049, 1095, 1124, 1146, 1257, 12741282, 1309, 1334, 1418, 1427, 1455, 1493, 1569, 1586, 16051668, 1712, 1722, 1739, 1822, 1849, 1890, 1913, 1929, 24292467, 2530, 2540, 2630, 2696, 2705, 2735, 2776, 2788, 31513180, 3223, 3234, 3299, 3378, 3403, 3408, 3454, 3544, 36423668, 3686, 3697, 3770, 3789M.CviTI (75)rgcy41, 58, 122, 144, 165, 217, 239, 255, 296, 378398, 432, 578, 592, 606, 687, 829, 849, 901, 930970, 978, 1023, 1034, 1049, 1095, 1124, 1146, 1257, 12741282, 1309, 1334, 1418, 1427, 1455, 1493, 1569, 1586, 16051668, 1712, 1722, 1739, 1822, 1849, 1890, 1913, 1929, 24292467, 2530, 2540, 2630, 2696, 2705, 2735, 2776, 2788, 31513180, 3223, 3234, 3299, 3378, 3403, 3408, 3454, 3544, 36423668, 3686, 3697, 3770, 3789M.NgoMXV (56)gcchr40, 61, 90, 134, 143, 295, 332, 381, 686, 9771033, 1058, 1292, 1430, 1440, 1492, 1503, 1738, 1889, 19171928, 2286, 2612, 2708, 2827, 3179, 3233, 3377, 3685, 36969, 236, 376, 400, 434, 519, 533, 831, 932, 10931159, 1353, 1614, 1720, 1736, 1756, 1793, 2431, 2574, 27783264, 3307, 3405, 3562, 3644, 3658AciI (44)ccgc88, 191, 462, 476, 915, 1060, 1416, 1480, 1581, 18641919, 2076, 2185, 2262, 2427, 2664, 2755, 3126, 3371, 34923511, 3638, 3666, 3828, 22, 32, 401, 507, 534, 9371156, 1259, 1322, 1824, 1869, 1935, 1961, 2306, 2473, 31173261, 3757, 3778, 3785BthCI (34)gcngc57, 241, 254, 358, 431, 463, 477, 499, 903, 11561259, 1311, 1322, 1412, 1454, 1495, 1582, 1588, 1824, 19201961, 1975, 2077, 2306, 2401, 2428, 2767, 3095, 3301, 33693512, 3667, 3785, 3806Fnu4HI (34)gcngc57, 241, 254, 358, 431, 463, 477, 499, 903, 11561259, 1311, 1322, 1412, 1454, 1495, 1582, 1588, 1824, 19201961, 1975, 2077, 2306, 2401, 2428, 2767, 3095, 3301, 33693512, 3667, 3785, 3806HhaI (30)gcgc6, 34, 99, 428, 452, 465, 474, 496, 522, 530894, 1004, 1102, 1173, 1342, 1350, 1414, 1451, 1717, 17471977, 2260, 2597, 2690, 3083, 3192, 3366, 3466, 3533, 3803HinP1I (30)gcgc6, 34, 99, 428, 452, 465, 474, 496, 522, 530894, 1004, 1102, 1173, 1342, 1350, 1414, 1451, 1717, 17471977, 2260, 2597, 2690, 3083, 3192, 3366, 3466, 3533, 3803M.HhaI (30)gcgc6, 34, 99, 428, 452, 465, 474, 496, 522, 530894, 1004, 1102, 1173, 1342, 1350, 1414, 1451, 1717, 17471977, 2260, 2597, 2690, 3083, 3192, 3366, 3466, 3533, 3803M.HinP1I (30)gcgc6, 34, 99, 428, 452, 465, 474, 496, 522, 530894, 1004, 1102, 1173, 1342, 1350, 1414, 1451, 1717, 17471977, 2260, 2597, 2690, 3083, 3192, 3366, 3466, 3533, 3803M.HpyAVIII (30)gcgc6, 34, 99, 428, 452, 465, 474, 496, 522, 530894, 1004, 1102, 1173, 1342, 1350, 1414, 1451, 1717, 17471977, 2260, 2597, 2690, 3083, 3192, 3366, 3466, 3533, 3803Sth132I (28)cccg89, 480, 602, 1439, 1481, 1514, 2285, 2818, 3332, 33763385, 3584, 31, 76, 400, 533, 585, 956, 1353, 17561895, 1966, 2211, 2272, 2635, 2794, 3039, 3477BstKTI (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144ChaI (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144DpnI (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.AacDam (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.AlaK2I (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.AvaV (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.AvaVI (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.BspKT6I (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.CviAI (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.DpnIIA (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.DpnIIB (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.Eco67Dam (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.EcoKDam (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.EcoP1Dam (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.EcoT1Dam (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.EcoT2Dam (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.EcoT4Dam (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.EsaLHCI (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.HindDam (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.HinHP1Dam (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.Hpy99VI (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.HpyAIII (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.LlaDCHIA (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.LlaDCHIB (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.LlaKR2I (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.MboIA (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.MboIB (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.PgiI (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.PhiCh1I (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.Sau3AI (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.SmaII (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.Sth368I (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.Sty1344Dam (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.StyDam (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144M.TpaI (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144MboI (24)gatc408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144Cac8I (23)gcnngc61, 90, 377, 401, 520, 534, 577, 1002, 1026, 10771094, 1244, 1430, 1649, 1715, 1749, 1794, 1852, 2708, 30993659, 3696, 3782AluI (22)agct58, 122, 217, 239, 255, 378, 592, 849, 970, 10231049, 1095, 1455, 1913, 2467, 2530, 2630, 3151, 3408, 34543544, 3770HpyF10VI (22)gcnnnnnnngc15, 99, 143, 410, 440, 472, 516, 543, 573, 9031042, 1342, 1426, 1449, 1588, 1711, 1747, 1794, 2704, 30923664, 3778M.AluI (22)agct58, 122, 217, 239, 255, 378, 592, 849, 970, 10231049, 1095, 1455, 1913, 2467, 2530, 2630, 3151, 3408, 34543544, 3770M.EsaBC1I (22)agct58, 122, 217, 239, 255, 378, 592, 849, 970, 10231049, 1095, 1455, 1913, 2467, 2530, 2630, 3151, 3408, 34543544, 3770MwoI (22)gcnnnnnnngc15, 99, 143, 410, 440, 472, 516, 543, 573, 9031042, 1342, 1426, 1449, 1588, 1711, 1747, 1794, 2704, 30923664, 3778HpaII (21)ccgg163, 576, 957, 1084, 1255, 1332, 1354, 1382, 1513, 16031670, 1851, 2284, 2526, 2636, 2703, 2737, 3141, 3331, 33573504M.BsuFI (21)ccgg163, 576, 957, 1084, 1255, 1332, 1354, 1382, 1513, 16031670, 1851, 2284, 2526, 2636, 2703, 2737, 3141, 3331, 33573504M.CboI (21)ccgg163, 576, 957, 1084, 1255, 1332, 1354, 1382, 1513, 16031670, 1851, 2284, 2526, 2636, 2703, 2737, 3141, 3331, 33573504M.HapII (21)ccgg163, 576, 957, 1084, 1255, 1332, 1354, 1382, 1513, 16031670, 1851, 2284, 2526, 2636, 2703, 2737, 3141, 3331, 33573504M.HpaII (21)ccgg163, 576, 957, 1084, 1255, 1332, 1354, 1382, 1513, 16031670, 1851, 2284, 2526, 2636, 2703, 2737, 3141, 3331, 33573504M.MjaVI (21)ccgg163, 576, 957, 1084, 1255, 1332, 1354, 1382, 1513, 16031670, 1851, 2284, 2526, 2636, 2703, 2737, 3141, 3331, 33573504M.MspI (21)ccgg163, 576, 957, 1084, 1255, 1332, 1354, 1382, 1513, 16031670, 1851, 2284, 2526, 2636, 2703, 2737, 3141, 3331, 33573504BbvI (20)gcagc241, 903, 1311, 1495, 1588, 2401, 3095, 3301, 57, 254358, 431, 499, 1412, 1454, 1975, 2767, 3369, 3788, 3806Hpy188III (20)tcnnga77, 316, 985, 1185, 1196, 1369, 1685, 1694, 1777, 18331877, 1967, 2000, 2732, 2991, 3065, 3148, 3246, 3344, 3478M.BbvI (20)gcagc241, 903, 1311, 1495, 1588, 2401, 3095, 3301, 57, 254358, 431, 499, 1412, 1454, 1975, 2767, 3369, 3788, 3806M.BbvSI (20)gcwgc57, 241, 254, 358, 431, 499, 903, 1311, 1412, 14541495, 1588, 1975, 2401, 2767, 3095, 3301, 3369, 3788, 3806TseI (20)gcwgc57, 241, 254, 358, 431, 499, 903, 1311, 1412, 14541495, 1588, 1975, 2401, 2767, 3095, 3301, 3369, 3788, 3806BsrI (18)actgg933, 2347, 3295, 3308, 81, 294, 320, 765, 975, 10561140, 1290, 1491, 2172, 2611, 2654, 2772, 3178M.EsaDix4I (18)ttaa51, 110, 247, 346, 470, 741, 839, 856, 867, 8792242, 2607, 2646, 2881, 2934, 2948, 2953, 3005M.EsaDix5I (18)ttaa51, 110, 247, 346, 470, 741, 839, 856, 867, 8792242, 2607, 2646, 2881, 2934, 2948, 2953, 3005M.MseI (18)ttaa51, 110, 247, 346, 470, 741, 839, 856, 867, 8792242, 2607, 2646, 2881, 2934, 2948, 2953, 3005MseI (18)ttaa51, 110, 247, 346, 470, 741, 839, 856, 867, 8792242, 2607, 2646, 2881, 2934, 2948, 2953, 3005M.Hpy99VA (17)cctc396, 1208, 1272, 1408, 1765, 2455, 2661, 3539, 3822, 25643, 1106, 1941, 2791, 2872, 3272, 3596M.MnlIA (17)cctc396, 1208, 1272, 1408, 1765, 2455, 2661, 3539, 3822, 25643, 1106, 1941, 2791, 2872, 3272, 3596M.MnlIB (17)cctc396, 1208, 1272, 1408, 1765, 2455, 2661, 3539, 3822, 25643, 1106, 1941, 2791, 2872, 3272, 3596MnlI (17)cctc396, 1208, 1272, 1408, 1765, 2455, 2661, 3539, 3822, 25643, 1106, 1941, 2791, 2872, 3272, 3596HaeIII (16)ggcc41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697M.AvaVII (16)ggcc41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697M.BflBF4I (16)gcsgc463, 477, 1156, 1259, 1322, 1417, 1582, 1824, 1920, 19612077, 2306, 2428, 3512, 3667, 3785M.BspRI (16)ggcc41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697M.BsuRI (16)ggcc41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697M.CglASI (16)gcsgc463, 477, 1156, 1259, 1322, 1417, 1582, 1824, 1920, 19612077, 2306, 2428, 3512, 3667, 3785M.HaeIII (16)ggcc41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697M.MthTI (16)ggcc41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697M.NgoGII (16)ggcc41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697M.NgoPII (16)ggcc41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697M.Phi3TI (16)ggcc41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697M.Rho11sI (16)ggcc41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697M.SPBetaI (16)ggcc41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697M.SPRI (16)ggcc41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697TauI (16)gcsgc463, 477, 1156, 1259, 1322, 1417, 1582, 1824, 1920, 19612077, 2306, 2428, 3512, 3667, 3785HpyCH4V (15)tgca55, 356, 1027, 1129, 1241, 1400, 1575, 1589, 2150, 23942482, 2675, 2765, 3100, 3396M.CviRI (15)tgca55, 356, 1027, 1129, 1241, 1400, 1575, 1589, 2150, 23942482, 2675, 2765, 3100, 3396M.CviSI (15)tgca55, 356, 1027, 1129, 1241, 1400, 1575, 1589, 2150, 23942482, 2675, 2765, 3100, 3396AlwI (14)ggatc1601, 2193, 2973, 3070, 1164, 1181, 1211, 1522, 1768, 21752496, 2960, 3058, 3144Hpy188I (14)tcnga622, 1167, 1190, 1316, 1660, 2013, 2322, 2442, 2453, 28993034, 3168, 3521, 3599M.AlwI (14)ggatc1601, 2193, 2973, 3070, 1164, 1181, 1211, 1522, 1768, 21752496, 2960, 3058, 3144M.Hpy188I (14)tcnga622, 1167, 1190, 1316, 1660, 2013, 2322, 2442, 2453, 28993034, 3168, 3521, 3599MaeIII (14)gtnac317, 337, 492, 504, 1472, 1778, 2162, 2350, 2503, 25612892, 3175, 3291, 3354CviAII (13)catg226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710FatI (13)catg226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710HpyCH4I (13)catg226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710M.CviAII (13)catg226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710M.CviQVII (13)catg226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710M.CviSII (13)catg226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710M.EsaBS1I (13)catg226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710M.Hpy166IV (13)catg226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710M.Hpy188II (13)catg226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710M.Hpy99X (13)catg226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710M.HpyAI (13)catg226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710M.HpyI (13)catg226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710M.NgoMV (13)ggnncc137, 608, 620, 641, 1103, 1351, 1386, 2524, 2735, 27762870, 3642, 3681M.NlaIII (13)catg226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710M.Sse9I (13)aatt108, 183, 200, 261, 288, 864, 875, 886, 1088, 20162389, 2644, 2950NlaIII (13)catg226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710NlaIV (13)ggnncc137, 608, 620, 641, 1103, 1351, 1386, 2524, 2735, 27762870, 3642, 3681Tsp509I (13)aatt108, 183, 200, 261, 288, 864, 875, 886, 1088, 20162389, 2644, 2950BstUI (12)cgcg33, 451, 475, 495, 871, 1415, 1716, 1748, 2261, 27543084, 3665M.BepI (12)cgcg33, 451, 475, 495, 871, 1415, 1716, 1748, 2261, 27543084, 3665M.BsuEII (12)cgcg33, 451, 475, 495, 871, 1415, 1716, 1748, 2261, 27543084, 3665M.DsaV (12)ccngg142, 331, 957, 1354, 1514, 1737, 2285, 2636, 3332, 35503563, 3684M.Ecl18kI (12)ccngg142, 331, 957, 1354, 1514, 1737, 2285, 2636, 3332, 35503563, 3684M.FnuDII (12)cgcg33, 451, 475, 495, 871, 1415, 1716, 1748, 2261, 27543084, 3665M.NlaX (12)ccngg142, 331, 957, 1354, 1514, 1737, 2285, 2636, 3332, 35503563, 3684M.SenPI (12)ccngg142, 331, 957, 1354, 1514, 1737, 2285, 2636, 3332, 35503563, 3684M.SsoII (12)ccngg142, 331, 957, 1354, 1514, 1737, 2285, 2636, 3332, 35503563, 3684M.TspRI (12)castg94, 293, 1030, 1474, 2397, 2424, 2771, 2876, 3025, 32963309, 3815ScrFI (12)ccngg142, 331, 957, 1354, 1514, 1737, 2285, 2636, 3332, 35503563, 3684SelI (12)cgcg33, 451, 475, 495, 871, 1415, 1716, 1748, 2261, 27543084, 3665StyD4I (12)ccngg142, 331, 957, 1354, 1514, 1737, 2285, 2636, 3332, 35503563, 3684TspRI (12)castg94, 293, 1030, 1474, 2397, 2424, 2771, 2876, 3025, 32963309, 3815HpyCH4III (11)acngt423, 705, 946, 1729, 1845, 1953, 2380, 2895, 3208, 36783749HpyCH4IV (11)acgt311, 569, 679, 722, 734, 922, 1466, 1653, 2219, 25923008M.Hpy99XI (11)acgt311, 569, 679, 722, 734, 922, 1466, 1653, 2219, 25923008M.HpyAIIA (11)gaaga550, 2010, 3061, 3213, 394, 1700, 1910, 2028, 2137, 22152970M.HpyCH4IV (11)acgt311, 569, 679, 722, 734, 922, 1466, 1653, 2219, 25923008M.MboIIA (11)gaaga550, 2010, 3061, 3213, 394, 1700, 1910, 2028, 2137, 22152970MboII (11)gaaga550, 2010, 3061, 3213, 394, 1700, 1910, 2028, 2137, 22152970TaiI (11)acgt311, 569, 679, 722, 734, 922, 1466, 1653, 2219, 25923008BccI (10)ccatc672, 1144, 1170, 1235, 1663, 2371, 2658, 2782, 690, 1563BscAI (10)gcatc1319, 1574, 1790, 2131, 2571, 3623, 1641, 1705, 1980, 2361BstF5I (10)ggatg364, 1533, 960, 1143, 1205, 1662, 1687, 2370, 2657, 2838BstYI (10)rgatcy1165, 1188, 1523, 1769, 2176, 2193, 2961, 2973, 3059, 3070FokI (10)ggatg364, 1533, 960, 1143, 1205, 1662, 1687, 2370, 2657, 2838HinfI (10)gantc46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811HpyAV (10)ccttc1485, 2463, 3171, 3481, 415, 555, 1986, 1996, 2088, 2700M.BabI (10)gantc46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811M.BspST5I (10)gcatc1319, 1574, 1790, 2131, 2571, 3623, 1641, 1705, 1980, 2361M.BstF5IA (10)ggatg364, 1533, 960, 1143, 1205, 1662, 1687, 2370, 2657, 2838M.BstF5ID (10)ggatg364, 1533, 960, 1143, 1205, 1662, 1687, 2370, 2657, 2838M.BstYI (10)rgatcy1165, 1188, 1523, 1769, 2176, 2193, 2961, 2973, 3059, 3070M.CcrMI (10)gantc46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811M.CviBI (10)gantc46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811M.CviQVI (10)gantc46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811M.FokI (10)ggatg364, 1533, 960, 1143, 1205, 1662, 1687, 2370, 2657, 2838M.HhaII (10)gantc46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811M.HinfI (10)gantc46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811M.Hpy99IX (10)gantc46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811M.HpyAIV (10)gantc46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811M.SscL1I (10)gantc46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811M.StsI (10)ggatg364, 1533, 960, 1143, 1205, 1662, 1687, 2370, 2657, 2838M.XhoII (10)rgatcy1165, 1188, 1523, 1769, 2176, 2193, 2961, 2973, 3059, 3070SfaNI (10)gcatc1319, 1574, 1790, 2131, 2571, 3623, 1641, 1705, 1980, 2361StsI (10)ggatg364, 1533, 960, 1143, 1205, 1662, 1687, 2370, 2657, 2838BslI (9)ccnnnnnnngg39, 462, 788, 1332, 1876, 3232, 3511, 3677, 3695Bsp1286I (9)gdgchc607, 1294, 1387, 1461, 1651, 1713, 2151, 2236, 3397Hpy99I (9)cgwcg300, 313, 1437, 1465, 1759, 1774, 2553, 2816, 3610EsaBC3I (8)tcga645, 1463, 1619, 1643, 1679, 1841, 2167, 3611M.CviBIII (8)tcga645, 1463, 1619, 1643, 1679, 1841, 2167, 3611M.CviQIII (8)tcga645, 1463, 1619, 1643, 1679, 1841, 2167, 3611M.CviSIII (8)tcga645, 1463, 1619, 1643, 1679, 1841, 2167, 3611M.EsaBC3I (8)tcga645, 1463, 1619, 1643, 1679, 1841, 2167, 3611M.EsaDix3I (8)tcga645, 1463, 1619, 1643, 1679, 1841, 2167, 3611M.HpyAX (8)tcga645, 1463, 1619, 1643, 1679, 1841, 2167, 3611M.Phi3TII (8)tcga645, 1463, 1619, 1643, 1679, 1841, 2167, 3611M.TaqI (8)tcga645, 1463, 1619, 1643, 1679, 1841, 2167, 3611M.TthHB8I (8)tcga645, 1463, 1619, 1643, 1679, 1841, 2167, 3611TaqI (8)tcga645, 1463, 1619, 1643, 1679, 1841, 2167, 3611BsaJI (7)ccnngg142, 331, 957, 1161, 1515, 1784, 3551CdiI (7)catcg1789, 2570, 691, 1637, 1840, 1981, 2166EaeI (7)yggccr42, 297, 1258, 1432, 1823, 1850, 2430FmuI (7)ggncc399, 687, 1867, 2458, 2680, 2697, 2776GdiII (7)cggccr42, 297, 1258, 1823, 1850, 1258, 2430Hpy8I (7)gtnnac677, 732, 749, 1137, 2151, 2909, 3397M.Cfr13I (7)ggncc399, 687, 1867, 2458, 2680, 2697, 2776M.CfrI (7)yggccr42, 297, 1258, 1432, 1823, 1850, 2430M.EaeI (7)yggccr42, 297, 1258, 1432, 1823, 1850, 2430M.EcoHK31I (7)yggccr42, 297, 1258, 1432, 1823, 1850, 2430M.Hpy8I (7)gtnnac677, 732, 749, 1137, 2151, 2909, 3397M.HpyAIX (7)gtnnac677, 732, 749, 1137, 2151, 2909, 3397M.PspPI (7)ggncc399, 687, 1867, 2458, 2680, 2697, 2776M.Sau96I (7)ggncc399, 687, 1867, 2458, 2680, 2697, 2776MspA1I (7)cmgckg59, 379, 1096, 1456, 2185, 3126, 3371Sau96I (7)ggncc399, 687, 1867, 2458, 2680, 2697, 2776TspDTI (7)atgaa48, 1838, 2942, 962, 1395, 2537, 2840UnbI (7)ggncc399, 687, 1867, 2458, 2680, 2697, 2776BanI (6)ggyrcc137, 641, 1103, 1351, 1386, 2870BsiEI (6)cgrycg409, 1258, 2302, 2451, 3374, 3798BstNI (6)ccwgg142, 331, 1737, 3550, 3563, 3684CjeI (6)ccannnnnngt3129, 3684, 383, 3030, 3130, 3208EcoHI (6)ccsgg957, 1354, 1514, 2285, 2636, 3332FauI (6)cccgc89, 480, 1481, 32, 401, 534HaeII (6)rgcgcy7, 523, 531, 1103, 1351, 3467Hin4I (6)gaynnnnnvtc1529, 1559, 2749, 2823, 1529, 2823HphI (6)ggtga2103, 2344, 669, 2118, 2740, 2967LpnI (6)rgcgcy7, 523, 531, 1103, 1351, 3467M.BcnIA (6)ccsgg957, 1354, 1514, 2285, 2636, 3332M.BcnIB (6)ccsgg957, 1354, 1514, 2285, 2636, 3332M.BstNI (6)ccwgg142, 331, 1737, 3550, 3563, 3684M.Eco1831I (6)ccsgg957, 1354, 1514, 2285, 2636, 3332M.EcoHI (6)ccsgg957, 1354, 1514, 2285, 2636, 3332M.EcoKDcm (6)ccwgg142, 331, 1737, 3550, 3563, 3684M.EcoPI (6)agacc791, 1673, 2750, 1371, 2901, 3036M.EcoRII (6)ccwgg142, 331, 1737, 3550, 3563, 3684M.HgiCI (6)ggyrcc137, 641, 1103, 1351, 1386, 2870M.HphIA (6)ggtga2103, 2344, 669, 2118, 2740, 2967M.HphIB (6)ggtga2103, 2344, 669, 2118, 2740, 2967M.Hpy99II (6)gtsac317, 504, 1472, 1778, 2350, 2561M.MlyI (6)gastc277, 729, 751, 2823, 3340, 3811M.MvaI (6)ccwgg142, 331, 1737, 3550, 3563, 3684M.NgoBVIIIA (6)ggtga2103, 2344, 669, 2118, 2740, 2967M.NgoHVIII (6)ggtga2103, 2344, 669, 2118, 2740, 2967M.StyLTI (6)cagag1108, 1315, 3252, 3278, 3598, 3189M.Tsp45I (6)gtsac317, 504, 1472, 1778, 2350, 2561MlyI (6)gagtc751, 3340, 277, 729, 2823, 3811NciI (6)ccsgg957, 1354, 1514, 2285, 2636, 3332PleI (6)gagtc751, 3340, 277, 729, 2823, 3811PspGI (6)ccwgg142, 331, 1737, 3550, 3563, 3684SfcI (6)ctryag271, 459, 1401, 2577, 3255, 3446SimI (6)gggtc648, 954, 1780, 3037, 2749, 3518SmlI (6)ctyrag248, 1743, 2198, 3066, 3343, 3605SspD5I (6)ggtga2103, 2344, 669, 2118, 2740, 2967Tsp45I (6)gtsac317, 504, 1472, 1778, 2350, 2561BceAI (5)acggc298, 1325, 3225, 638, 1761BcefI (5)acggc298, 1325, 3225, 638, 1761BfaI (5)ctag527, 1051, 2628, 2963, 3216BmrI (5)actggg321, 976, 1057, 1291, 2773BpuEI (5)cttgag1743, 3343, 2198, 3066, 3605BsaWI (5)wccggw1085, 1383, 2527, 3358, 3505BsiHKAI (5)gwgcwc1461, 1651, 2151, 2236, 3397Eco57MI (5)ctgrag3184, 1477, 1909, 2136, 2733M.BfiIA (5)actggg321, 976, 1057, 1291, 2773M.BfiIB (5)actggg321, 976, 1057, 1291, 2773M.MthZI (5)ctag527, 1051, 2628, 2963, 3216TaqII (5)gaccga790, 2301, 1373, 2460, 3799AcuI (4)ctgaag3184, 1477, 1909, 2136Bme1580I (4)gkgcmc1294, 1387, 2151, 3397BseMII (4)ctcag2861, 3027, 3436, 2321BspCNI (4)ctcag2861, 3027, 3436, 2321BsrBI (4)ccgctc191, 536, 1963, 3780BsrFI (4)rccggy577, 1671, 1852, 2738BtsI (4)gcagtg2425, 94, 1030, 2397CjePI (4)ccannnnnnntc3178, 2439, 2911, 3031DdeI (4)ctnag2321, 2861, 3027, 3436EarI (4)ctcttc395, 1701, 1911, 2029EciI (4)ggcgga1865, 2665, 3493, 3639HaeI (4)wggccw1432, 3235, 3687, 3698HgaI (4)gacgc3031, 3609, 450, 2281M.AvaIX (4)rccggy577, 1671, 1852, 2738M.Cfr10I (4)rccggy577, 1671, 1852, 2738M.Cfr6I (4)cagctg59, 379, 1096, 1456M.DdeI (4)ctnag2321, 2861, 3027, 3436M.Eco57IA (4)ctgaag3184, 1477, 1909, 2136M.Eco57IB (4)ctgaag3184, 1477, 1909, 2136M.HgaIA (4)gacgc3031, 3609, 450, 2281M.HgaIB (4)gacgc3031, 3609, 450, 2281M.PvuII (4)cagctg59, 379, 1096, 1456M.TfiI (4)gawtc46, 1836, 1970, 3736MslI (4)caynnnnrtg1789, 2052, 2411, 2570PvuII (4)cagctg59, 379, 1096, 1456TfiI (4)gawtc46, 1836, 1970, 3736Tth111II (4)caarca1797, 3098, 3137, 3104AceIII (3)cagctc850, 2531, 3771ApoI (3)raatty865, 876, 887AseI (3)attaat52, 111, 2647AvaII (3)ggwcc1867, 2458, 2680BsaAI (3)yacgtr680, 923, 1654BsaHI (3)grcgyc1103, 1351, 2282BseYI (3)cccagc1098, 1856, 3407BspMI (3)acctgc1019, 1248, 1611BsrDI (3)gcaatg2764, 1578, 2582BssSI (3)cacgag1944, 2154, 3538DraI (3)tttaaa2243, 2935, 2954DrdI (3)gacnnnnnngtc729, 1379, 3609FspI (3)tgcgca429, 1452, 2598M.BsaAI (3)yacgtr680, 923, 1654M.DraI (3)tttaaa2243, 2935, 2954M.EcoP15I (3)cagcag3094, 3303, 3187M.MmeIA (3)tccrac726, 3337, 3521M.MmeIB (3)tccrac726, 3337, 3521M.SinI (3)ggwcc1867, 2458, 2680M.VspI (3)attaat52, 111, 2647MmeI (3)tccrac726, 3337, 3521PpiI (3)gaacnnnnnctc736, 3020, 2177Psp03I (3)ggwcc1867, 2458, 2680VpaK11AI (3)ggwcc1867, 2458, 2680AclI (2)aacgtt2220, 2593AflIII (2)acrygt922, 3711AlwNI (2)cagnnnctg974, 3300ApaLI (2)gtgcac2151, 3397BanII (2)grgcyc607, 1713BbeI (2)ggcgcc1103, 1351BciVI (2)gtatcc1560, 3502BglI (2)gccnnnnnggc440, 2704BsaBI (2)gatnnnnatc1187, 1211BsaXI (2)acnnnnnctcc734, 28BsmAI (2)gtctc1199, 2751Csp6I (2)gtac1655, 2339FalI (2)aagnnnnnctt245, 1674HaeIV (2)gaynnnnnrtc1529, 2823KasI (2)ggcgcc1103, 1351M.AclI (2)aacgtt2220, 2593M.AflIII (2)acrygt922, 3711M.Alw26I (2)gtctc1199, 2751M.BanII (2)grgcyc607, 1713M.CfrBI (2)ccwwgg1161, 1784M.CviQI (2)gtac1655, 2339M.CviRII (2)gtac1655, 2339M.HaeIV (2)gaynnnnnrtc1529, 2823M.NgoBIX (2)gtannnnnctc2162, 3444M.NgoMIV (2)gccggc577, 1852M.NspHI (2)rcatgy1753, 3711M.NspI (2)rcatgy1753, 3711M.PhiMx8I (2)ctssag1401, 2733M.RsaI (2)gtac1655, 2339M.SauLPI (2)gccggc577, 1852NaeI (2)gccggc577, 1852NarI (2)ggcgcc1103, 1351NgoMIV (2)gccggc577, 1852NspI (2)rcatgy1753, 3711PvuI (2)cgatcg409, 2451RsaI (2)gtac1655, 2339SapI (2)gctcttc1702, 1912SfoI (2)ggcgcc1103, 1351StyI (2)ccwwgg1161, 1784TspGWI (2)acgga2051, 2368AflII (1)cttaag248AhdI (1)gacnnnnngtc2823AloI (1)gaacnnnnnntcc736ApaBI (1)gcannnnntgc903BcgI (1)cgannnnnntgc2302BclI (1)tgatca1193BglII (1)agatct1188BpmI (1)ctggag2733BsaI (1)ggtctc2751BsmFI (1)gggac1488Bsp24I (1)gacnnnnnntgg3031BspHI (1)tcatga2991BssHII (1)gcgcgc1749BstAPI (1)gcannnnntgc903BtgI (1)ccrygg1784DraIII (1)cacnnngtg683EagI (1)cggccg1258M.AloI (1)gaacnnnnnntcc736M.BcgI (1)cgannnnnntgc2302M.BclI (1)tgatca1193M.BglII (1)agatct1188M.BmeTI (1)tgatca1193M.BspLU11IIIA (1)gggac1488M.BspLU11IIIC (1)gggac1488M.BsuBI (1)ctgcag1401M.DraIII (1)cacnnngtg683M.EcoKI (1)aacnnnnnngtgc2160M.EcoR124II (1)gaannnnnnnrtcg1642M.NmeSI (1)agtact2340M.PstI (1)ctgcag1401M.ScaI (1)agtact2340M.StyLTIII (1)gagnnnnnnrtayg2319M.StySPI (1)aacnnnnnngtrc2160M.TaeII (1)tgatca1193M.Tth111I (1)gacnnngtc1470M.XcmI (1)ccannnnnnnnntgg1170M.XmaIII (1)cggccg1258MscI (1)tggcca1432PciI (1)acatgt3711PsiI (1)ttataa808PstI (1)ctgcag1401RsrII (1)cggwccg1868ScaI (1)agtact2340SphI (1)gcatgc1753TatI (1)wgtacw2340Tth111I (1)gacnnngtc1470XcmI (1)ccannnnnnnnntgg1170XmnI (1)gaannnnttc2223 Non-Cutters AarI, AatII, Acc65I, AccI, AfeI, AgeI, AleI, ApaI, AscI, AsiSI AvaI, AvrII, BaeI, BamHI, Bbr7I, BbsI, BbvCI, BfrBI, BlpI, BmgBI BmtI, BplI, Bpu10I, BseRI, BsgI, BsiWI, BsmBI, BsmI, BspEI, BsrGI BstBI, BstEII, BstXI, BstZ17I, Bsu36I, ClaI, EcoICRI, EcoNI, EcoO109I, EcoRI EcoRV, FseI, FspAI, HincII, HindIII, HpaI, KpnI, M.AccI, M.ApaI, M.AquI M.BaeI, M.BbvCIA, M.BbvCIB, M.BnaI, M.BseCI, M.Bsp106I, M.BstI, M.BstVI, M.Bsu15I, M.Cfr9I M.ClaI, M.EcaI, M.EcoAI, M.EcoBI, M.EcoO109I, M.EcoR124I, M.EcoRI, M.EcoRV, M.Esp3I, M.HincII M.HindII, M.HindIII, M.HpaI, M.KpnAI, M.KpnBI, M.KpnI, M.MunI, M.NgoAV, M.PaeR7I, M.PhiBssHII 45  # $ ) * 4 )*+ɻ~gWgB)h37CJKHOJQJ^JaJmH o(sH h37CJKHOJQJ^JaJ,hh37CJKHOJQJ^JaJmH sH 2hh376CJKHOJQJ]^JaJmH sH h6Kh7 F5o(h6Kh6K5o( hwo(h7 FB*o(ph *h+`h7 FB*o(phhPh7 FB*o(phhPh7 FB*ph h+`h7 F hph7 F h7 Fo( h7 F5 h7 F5o(45,-./T01\xyz{|}~ $7$8$H$a$gd37gd6K .0n~'0R[\mwxEл移ЗллЧzsnse`\Lh7 FCJKHOJQJ^JaJh7 F h7 Fo(h7 F5KHaJ h7 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la<VWdjzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd $$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<Ăzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd6$$IflF@  ppp0    4 la< zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la< Vzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd`$$IflF@  ppp0    4 la<VWXYdzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<depvzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<ǃ̃zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdI$$IflF@  ppp0    4 la<"Zzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<Z[\]hzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kds$$IflF@  ppp0    4 la<hitzzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<Єքzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd2$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<%*_zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd\$$IflF@  ppp0    4 la<_`abgzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<ghw|zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd $$IflF@  ppp0    4 la<Ʌυzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd $$IflF@  ppp0    4 la< zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdE!$$IflF@  ppp0    4 la<!&[zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd!$$IflF@  ppp0    4 la<[\]^czk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdo"$$IflF@  ppp0    4 la<cdrxzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd#$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd#$$IflF@  ppp0    4 la<ņˆzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd.$$$IflF@  ppp0    4 la< zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd$$$IflF@  ppp0    4 la< Qzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdX%$$IflF@  ppp0    4 la<QRSTYzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd%$$IflF@  ppp0    4 la<YZdjzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd&$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd'$$IflF@  ppp0    4 la<<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd'$$IflF@  ppp0    4 la<<=HOzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdA($$IflF@  ppp0    4 la<Ԉzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd($$IflF@  ppp0    4 la<ԈՈzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdk)$$IflF@  ppp0    4 la<(.gzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd*$$IflF@  ppp0    4 la<ghtzzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd*$$IflF@  ppp0    4 la<Ɖzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd*+$$IflF@  ppp0    4 la<Pzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd+$$IflF@  ppp0    4 la<PQ`fzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdT,$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd,$$IflF@  ppp0    4 la<9zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd~-$$IflF@  ppp0    4 la<9:GMzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd.$$IflF@  ppp0    4 la<΋zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd.$$IflF@  ppp0    4 la<΋ϋۋzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd=/$$IflF@  ppp0    4 la<(.dzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd/$$IflF@  ppp0    4 la<derxzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdg0$$IflF@  ppp0    4 la<Čzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd0$$IflF@  ppp0    4 la< Ezk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd1$$IflF@  ppp0    4 la<EFTZzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd&2$$IflF@  ppp0    4 la<܍zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd2$$IflF@  ppp0    4 la<܍ݍ,zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdP3$$IflF@  ppp0    4 la<,-8>yzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd3$$IflF@  ppp0    4 la<yzÎzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdz4$$IflF@  ppp0    4 la<ÎĎَ͎ zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd5$$IflF@  ppp0    4 la< Szk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd5$$IflF@  ppp0    4 la<ST_ezk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd96$$IflF@  ppp0    4 la<؏zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd6$$IflF@  ppp0    4 la<؏ُzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdc7$$IflF@  ppp0    4 la<).\zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd7$$IflF@  ppp0    4 la<\]kpzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd8$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd"9$$IflF@  ppp0    4 la<#zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd9$$IflF@  ppp0    4 la<#$05czk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdL:$$IflF@  ppp0    4 la<cdrwzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd:$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdv;$$IflF@  ppp0    4 la<&zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd <$$IflF@  ppp0    4 la<&'05czk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd<$$IflF@  ppp0    4 la<cdnuzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd5=$$IflF@  ppp0    4 la<Ӓzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd=$$IflF@  ppp0    4 la<ӒԒݒ zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd_>$$IflF@  ppp0    4 la< Azk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd>$$IflF@  ppp0    4 la<ABLSyzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd?$$IflF@  ppp0    4 la<yzzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd@$$IflF@  ppp0    4 la<œzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd@$$IflF@  ppp0    4 la<%zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdHA$$IflF@  ppp0    4 la<%&18^zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdA$$IflF@  ppp0    4 la<^_nuzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdrB$$IflF@  ppp0    4 la<Քzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdC$$IflF@  ppp0    4 la<Ք֔zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdC$$IflF@  ppp0    4 la<#Jzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd1D$$IflF@  ppp0    4 la<JKX^zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdD$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd[E$$IflF@  ppp0    4 la<ʕЕzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdE$$IflF@  ppp0    4 la< /zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdF$$IflF@  ppp0    4 la</09?fzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdG$$IflF@  ppp0    4 la<fgpwzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdG$$IflF@  ppp0    4 la<̖zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdDH$$IflF@  ppp0    4 la<̖͖זݖzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdH$$IflF@  ppp0    4 la<6zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdnI$$IflF@  ppp0    4 la<67AGizk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdJ$$IflF@  ppp0    4 la<ijsyzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdJ$$IflF@  ppp0    4 la<ŗzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd-K$$IflF@  ppp0    4 la<ŗƗЗܗzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdK$$IflF@  ppp0    4 la< 1zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdWL$$IflF@  ppp0    4 la<12;B`zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdL$$IflF@  ppp0    4 la<`amszk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdM$$IflF@  ppp0    4 la<ʘzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdN$$IflF@  ppp0    4 la<ʘ˘טݘzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdN$$IflF@  ppp0    4 la<6zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd@O$$IflF@  ppp0    4 la<67CIkzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdO$$IflF@  ppp0    4 la<klzzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdjP$$IflF@  ppp0    4 la<֙zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdP$$IflF@  ppp0    4 la<֙י zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdQ$$IflF@  ppp0    4 la< @zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd)R$$IflF@  ppp0    4 la<@AMSuzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdR$$IflF@  ppp0    4 la<uvzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdSS$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdS$$IflF@  ppp0    4 la<zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd}T$$IflF@  ppp0    4 la<$Ezk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdU$$IflF@  ppp0    4 la<EFV\~zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdU$$IflF@  ppp0    4 la<~zk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kdzk\k$$1$7$8$H$Ifa$$$1$7$8$H$Ifa$kd|{$$IflF@  ppp0    4 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