ࡱ>  {}rstuvwxyzg RbjbjVV=r<r< ,,,,,@@@8x$@Y@,@,@,@,@,t-t-t-XXXXXXX$k[^L Y9,z.t-t-z.z. Y,,@,@,4YYPVPVPVz.&,@,,@,XPVz.XPVPVPV@,HCUPVXoY0YPVi^U^i^PVPV&i^,vVpt--PV- -t-t-t- Y YUXt-t-t-Yz.z.z.z.i^t-t-t-t-t-t-t-t-t-, :  pEASY-T5 Zero Complete sequence AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCAGAATTAACCCTCACTAAAGGGACTAGTCCTGCAGGTTTAAACGAATTGGCCCTTAGGGCCAATTCGCGGCCGCTAAATTCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTATACGTACGGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCGGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTCACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGCTCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTATTAACGCTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG M13F5'-GTAAAACGACGGCCAGT-3' vU_SGJ101-01 M13R5'-CAGGAAACAGCTATGAC-3' vU_SGJ101-11 LacZa-ccdB fragment: bases 217-807 M13 reverse priming site: bases 205-221 T7 promoter/priming site: bases 325-344 M13 Forward priming site: bases 352-367 Kanamycin resistance ORF: bases 1156-1950 Ampicillin resistance ORF: bases 2200-3060 pUC origin: bases 3158-3831 pEASY-T5 Zero Cutters M.CviQII (351)rar25, 46, 72, 123, 127, 145, 152, 159, 166, 201240, 253, 279, 294, 304, 319, 324, 351, 365, 406415, 431, 476, 511, 518, 553, 642, 652, 723, 751796, 840, 853, 962, 973, 1001, 1044, 1085, 1089, 11251153, 1188, 1199, 1216, 1251, 1267, 1295, 1299, 1382, 13991462, 1472, 1477, 1481, 1534, 1555, 1595, 1612, 1651, 16691678, 1708, 1734, 1764, 1774, 1852, 1875, 1879, 1885, 19011908, 1923, 1933, 1945, 1964, 1971, 1986, 1990, 2012, 20342064, 2068, 2072, 2086, 2102, 2125, 2138, 2158, 2190, 22122233, 2249, 2254, 2273, 2308, 2353, 2361, 2368, 2424, 24342479, 2494, 2539, 2546, 2556, 2561, 2566, 2572, 2629, 26332640, 2681, 2707, 2711, 2737, 2758, 2771, 2803, 2850, 28612878, 2894, 2905, 2926, 2940, 2958, 2967, 2973, 2985, 30593065, 3071, 3075, 3093, 3110, 3126, 3137, 3143, 3183, 31953199, 3220, 3255, 3259, 3282, 3286, 3304, 3333, 3364, 33733395, 3399, 3449, 3463, 3514, 3532, 3596, 3668, 3692, 37103719, 3725, 3737, 3748, 3793, 3803, 3811, 3822, 3828, 38363840, 3859, 3883, 3903, 18, 83, 96, 116, 181, 188211, 237, 244, 259, 282, 286, 316, 337, 385, 454514, 529, 533, 537, 567, 637, 649, 679, 736, 740763, 775, 803, 809, 822, 831, 864, 879, 900, 927931, 939, 945, 950, 1014, 1018, 1024, 1051, 1059, 10691074, 1098, 1122, 1133, 1145, 1165, 1169, 1207, 1306, 13291333, 1340, 1345, 1414, 1421, 1444, 1489, 1493, 1565, 15691582, 1586, 1603, 1636, 1663, 1681, 1686, 1702, 1740, 17531846, 1861, 1942, 1952, 2043, 2055, 2079, 2089, 2107, 21162143, 2148, 2154, 2162, 2172, 2183, 2187, 2222, 2303, 23432380, 2394, 2402, 2409, 2462, 2469, 2473, 2584, 2589, 26152619, 2655, 2660, 2731, 2753, 2765, 2775, 2797, 2847, 28542871, 2889, 2899, 2917, 2994, 2998, 3006, 3012, 3022, 30263030, 3034, 3046, 3054, 3087, 3119, 3134, 3150, 3167, 31763189, 3225, 3229, 3233, 3270, 3292, 3312, 3325, 3368, 34403466, 3485, 3504, 3536, 3540, 3554, 3559, 3575, 3582, 35863602, 3607, 3611, 3631, 3645, 3651, 3661, 3665, 3678, 37283763, 3772, 3887, 3891, 3915, 3923, 3932, 3940, 3944, 39483953M.CviPI (297)gc4, 15, 22, 32, 40, 54, 57, 61, 86, 9097, 104, 121, 134, 143, 157, 164, 189, 216, 233238, 273, 290, 299, 306, 309, 312, 326, 357, 394412, 417, 420, 435, 439, 443, 451, 459, 463, 472488, 493, 506, 538, 584, 590, 612, 618, 626, 665682, 686, 708, 716, 744, 754, 760, 780, 817, 867875, 886, 907, 915, 936, 940, 953, 960, 964, 971980, 986, 996, 1011, 1015, 1028, 1032, 1038, 1061, 10661083, 1091, 1094, 1109, 1123, 1156, 1178, 1182, 1194, 11971211, 1219, 1230, 1246, 1249, 1257, 1260, 1271, 1278, 12861323, 1337, 1347, 1350, 1355, 1364, 1368, 1378, 1387, 13921397, 1416, 1430, 1433, 1441, 1448, 1454, 1479, 1485, 15061512, 1517, 1520, 1523, 1526, 1532, 1542, 1549, 1566, 15761583, 1587, 1605, 1640, 1649, 1653, 1659, 1671, 1676, 16831687, 1691, 1699, 1723, 1728, 1732, 1736, 1759, 1762, 17861790, 1800, 1807, 1821, 1827, 1842, 1850, 1855, 1858, 18661873, 1883, 1896, 1899, 1910, 1913, 1920, 1930, 1962, 19791997, 2005, 2020, 2030, 2049, 2100, 2210, 2224, 2236, 22662306, 2318, 2326, 2329, 2340, 2351, 2359, 2374, 2385, 23892398, 2403, 2419, 2431, 2456, 2464, 2481, 2496, 2509, 25152522, 2537, 2554, 2564, 2586, 2612, 2620, 2627, 2665, 26682692, 2695, 2700, 2721, 2735, 2787, 2790, 2801, 2807, 28122833, 2843, 2859, 2886, 2910, 2944, 2962, 2981, 2995, 30093016, 3019, 3037, 3089, 3151, 3204, 3215, 3218, 3222, 32403247, 3261, 3273, 3302, 3308, 3313, 3345, 3356, 3379, 33843397, 3402, 3421, 3424, 3427, 3434, 3487, 3492, 3500, 35183525, 3530, 3537, 3570, 3576, 3587, 3594, 3613, 3632, 36353654, 3666, 3682, 3713, 3729, 3746, 3759, 3764, 3778, 37823787, 3790, 3808, 3815, 3819, 3826, 3844, 3880, 3892, 39013905, 3908, 3911, 3924, 3929, 3941, 3949M.SssI (252)cg5, 14, 21, 31, 35, 42, 64, 76, 87, 98103, 162, 167, 190, 232, 284, 305, 311, 325, 340359, 362, 372, 375, 395, 411, 434, 444, 452, 462467, 471, 489, 500, 504, 540, 546, 583, 586, 591594, 621, 645, 658, 671, 687, 701, 718, 725, 731759, 779, 859, 874, 882, 894, 935, 941, 981, 9971016, 1021, 1039, 1093, 1110, 1150, 1155, 1181, 1192, 11961217, 1244, 1259, 1262, 1269, 1279, 1287, 1291, 1310, 13191343, 1351, 1360, 1372, 1375, 1379, 1388, 1400, 1403, 14171442, 1450, 1487, 1518, 1521, 1531, 1540, 1556, 1567, 15751580, 1584, 1590, 1596, 1607, 1616, 1634, 1652, 1661, 16701684, 1694, 1697, 1700, 1709, 1712, 1724, 1738, 1761, 17781788, 1801, 1806, 1822, 1833, 1856, 1859, 1872, 1880, 18891895, 1898, 1904, 1909, 1914, 1919, 1929, 1940, 1961, 19801996, 2006, 2019, 2038, 2050, 2099, 2237, 2251, 2277, 22802294, 2299, 2339, 2350, 2357, 2391, 2400, 2404, 2430, 24582480, 2497, 2502, 2526, 2536, 2540, 2543, 2568, 2578, 25872621, 2634, 2642, 2646, 2667, 2727, 2788, 2791, 2795, 28102813, 2821, 2832, 2875, 2882, 2887, 2909, 2927, 2941, 29823018, 3038, 3043, 3130, 3140, 3152, 3162, 3205, 3239, 32483263, 3290, 3314, 3347, 3383, 3396, 3435, 3443, 3453, 34693479, 3488, 3493, 3497, 3506, 3515, 3538, 3542, 3552, 35713588, 3593, 3600, 3614, 3626, 3633, 3642, 3655, 3659, 36813705, 3712, 3730, 3733, 3749, 3760, 3777, 3786, 3799, 38453871, 3879, 3900, 3904, 3907, 3913, 3917, 3921, 3925, 39303942, 3950M.CviAIV (242)rgcb7, 23, 41, 58, 122, 144, 165, 217, 234, 239310, 327, 358, 413, 436, 460, 473, 494, 539, 585613, 683, 709, 745, 755, 781, 868, 908, 916, 961972, 987, 1033, 1041, 1062, 1084, 1095, 1124, 1195, 12121220, 1247, 1261, 1272, 1289, 1356, 1365, 1393, 1431, 15071524, 1533, 1543, 1606, 1650, 1656, 1672, 1677, 1760, 17871808, 1828, 1851, 1867, 1874, 1897, 1921, 1931, 1963, 21012307, 2319, 2352, 2360, 2386, 2399, 2432, 2465, 2482, 25382555, 2565, 2628, 2666, 2836, 2911, 3241, 3274, 3303, 33463357, 3385, 3398, 3422, 3501, 3526, 3531, 3577, 3590, 35953667, 3683, 3714, 3765, 3779, 3791, 3809, 3820, 3881, 38933902, 3912, 5, 41, 58, 87, 98, 122, 144, 165190, 217, 239, 310, 358, 395, 440, 452, 460, 494539, 591, 613, 683, 709, 745, 755, 868, 908, 916941, 961, 972, 981, 987, 997, 1016, 1033, 1039, 10621084, 1110, 1124, 1195, 1212, 1220, 1247, 1272, 1279, 12871351, 1356, 1365, 1379, 1393, 1417, 1431, 1442, 1507, 15211543, 1567, 1584, 1606, 1650, 1660, 1677, 1684, 1700, 17241760, 1787, 1801, 1822, 1828, 1851, 1856, 1867, 1914, 22372307, 2319, 2360, 2386, 2399, 2404, 2465, 2555, 2565, 25872621, 2628, 2666, 2791, 2813, 2887, 2982, 3038, 3152, 32483274, 3303, 3314, 3346, 3357, 3422, 3435, 3488, 3493, 35013526, 3531, 3538, 3571, 3577, 3588, 3614, 3633, 3655, 36673730, 3760, 3765, 3791, 3809, 3820, 3893, 3912, 3925, 39303942, 3950M.CviJI (242)rgcb7, 23, 41, 58, 122, 144, 165, 217, 234, 239310, 327, 358, 413, 436, 460, 473, 494, 539, 585613, 683, 709, 745, 755, 781, 868, 908, 916, 961972, 987, 1033, 1041, 1062, 1084, 1095, 1124, 1195, 12121220, 1247, 1261, 1272, 1289, 1356, 1365, 1393, 1431, 15071524, 1533, 1543, 1606, 1650, 1656, 1672, 1677, 1760, 17871808, 1828, 1851, 1867, 1874, 1897, 1921, 1931, 1963, 21012307, 2319, 2352, 2360, 2386, 2399, 2432, 2465, 2482, 25382555, 2565, 2628, 2666, 2836, 2911, 3241, 3274, 3303, 33463357, 3385, 3398, 3422, 3501, 3526, 3531, 3577, 3590, 35953667, 3683, 3714, 3765, 3779, 3791, 3809, 3820, 3881, 38933902, 3912, 5, 41, 58, 87, 98, 122, 144, 165190, 217, 239, 310, 358, 395, 440, 452, 460, 494539, 591, 613, 683, 709, 745, 755, 868, 908, 916941, 961, 972, 981, 987, 997, 1016, 1033, 1039, 10621084, 1110, 1124, 1195, 1212, 1220, 1247, 1272, 1279, 12871351, 1356, 1365, 1379, 1393, 1417, 1431, 1442, 1507, 15211543, 1567, 1584, 1606, 1650, 1660, 1677, 1684, 1700, 17241760, 1787, 1801, 1822, 1828, 1851, 1856, 1867, 1914, 22372307, 2319, 2360, 2386, 2399, 2404, 2465, 2555, 2565, 25872621, 2628, 2666, 2791, 2813, 2887, 2982, 3038, 3152, 32483274, 3303, 3314, 3346, 3357, 3422, 3435, 3488, 3493, 35013526, 3531, 3538, 3571, 3577, 3588, 3614, 3633, 3655, 36673730, 3760, 3765, 3791, 3809, 3820, 3893, 3912, 3925, 39303942, 3950M.HindI (103)cac94, 114, 176, 186, 335, 377, 556, 569, 601, 617647, 661, 713, 742, 885, 968, 1204, 1264, 1322, 13621396, 1447, 1714, 1750, 1784, 1796, 1835, 1882, 2004, 20282048, 2220, 2282, 2325, 2412, 2528, 2611, 2672, 2744, 27512782, 2858, 2943, 2992, 3132, 3251, 3341, 3419, 3432, 34453517, 3573, 3742, 3863, 3889, 3938, 63, 125, 136, 150204, 255, 353, 422, 465, 522, 582, 620, 699, 757843, 858, 1180, 1232, 1371, 1410, 1474, 1561, 1589, 20152032, 2128, 2226, 2349, 2355, 2535, 2679, 2697, 2838, 29382946, 2977, 3042, 3146, 3237, 3359, 3381, 3520, 3550, 35923658, 3716, 3830CviJI (75)rgcy41, 58, 122, 144, 165, 217, 239, 310, 358, 440460, 494, 539, 613, 683, 709, 745, 755, 868, 908916, 961, 972, 987, 1033, 1062, 1084, 1124, 1195, 12121220, 1247, 1272, 1356, 1365, 1393, 1431, 1507, 1524, 15431606, 1650, 1660, 1677, 1760, 1787, 1828, 1851, 1867, 23072319, 2360, 2386, 2399, 2465, 2555, 2565, 2628, 2666, 32743303, 3346, 3357, 3422, 3501, 3526, 3531, 3577, 3667, 37653791, 3809, 3820, 3893, 3912M.CviTI (75)rgcy41, 58, 122, 144, 165, 217, 239, 310, 358, 440460, 494, 539, 613, 683, 709, 745, 755, 868, 908916, 961, 972, 987, 1033, 1062, 1084, 1124, 1195, 12121220, 1247, 1272, 1356, 1365, 1393, 1431, 1507, 1524, 15431606, 1650, 1660, 1677, 1760, 1787, 1828, 1851, 1867, 23072319, 2360, 2386, 2399, 2465, 2555, 2565, 2628, 2666, 32743303, 3346, 3357, 3422, 3501, 3526, 3531, 3577, 3667, 37653791, 3809, 3820, 3893, 3912M.NgoMXV (57)gcchr40, 61, 90, 134, 143, 357, 394, 443, 612, 686817, 915, 971, 996, 1230, 1368, 1378, 1430, 1441, 16761827, 1855, 1866, 2318, 2522, 2665, 3302, 3356, 3500, 38083819, 9, 236, 438, 462, 496, 615, 711, 870, 10311097, 1291, 1552, 1658, 1674, 1694, 1731, 2269, 2388, 24842810, 3387, 3430, 3528, 3685, 3767, 3781AciI (47)ccgc88, 191, 308, 998, 1354, 1418, 1519, 1802, 1857, 19812007, 2053, 2622, 2789, 3249, 3494, 3615, 3634, 3761, 37893951, 22, 32, 312, 463, 760, 875, 1094, 1197, 12601762, 1807, 1873, 1899, 2020, 2100, 2340, 2431, 2668, 28332910, 3019, 3240, 3384, 3880, 3901, 3908Sth132I (30)cccg89, 588, 673, 733, 1377, 1419, 1452, 2040, 2301, 24602823, 2884, 3455, 3499, 3508, 3707, 31, 76, 462, 645658, 894, 1291, 1694, 1833, 1904, 2277, 2810, 3162, 3600BthCI (29)gcngc57, 309, 420, 493, 1094, 1197, 1249, 1260, 1350, 13921433, 1520, 1526, 1762, 1858, 1899, 1913, 2329, 2668, 26952790, 3019, 3218, 3424, 3492, 3635, 3790, 3908, 3929Fnu4HI (29)gcngc57, 309, 420, 493, 1094, 1197, 1249, 1260, 1350, 13921433, 1520, 1526, 1762, 1858, 1899, 1913, 2329, 2668, 26952790, 3019, 3218, 3424, 3492, 3635, 3790, 3908, 3929BstKTI (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267ChaI (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267DpnI (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.AacDam (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.AlaK2I (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.AvaV (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.AvaVI (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.BspKT6I (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.CviAI (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.DpnIIA (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.DpnIIB (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.Eco67Dam (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.EcoKDam (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.EcoP1Dam (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.EcoT1Dam (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.EcoT2Dam (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.EcoT4Dam (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.EsaLHCI (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.HindDam (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.HinHP1Dam (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.Hpy99VI (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.HpyAIII (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.LlaDCHIA (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.LlaDCHIB (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.LlaKR2I (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.MboIA (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.MboIB (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.PgiI (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.PhiCh1I (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.Sau3AI (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.SmaII (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.Sth368I (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.Sty1344Dam (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.StyDam (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267M.TpaI (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267MboI (27)gatc470, 604, 749, 838, 850, 1102, 1119, 1130, 1149, 14601538, 1619, 1628, 1706, 2123, 2135, 2240, 2581, 2599, 26452903, 2920, 2956, 3173, 3181, 3192, 3267HhaI (24)gcgc6, 34, 99, 490, 688, 942, 1040, 1111, 1280, 12881352, 1389, 1655, 1915, 2051, 2405, 2498, 2835, 3206, 33153489, 3589, 3656, 3926HinP1I (24)gcgc6, 34, 99, 490, 688, 942, 1040, 1111, 1280, 12881352, 1389, 1655, 1915, 2051, 2405, 2498, 2835, 3206, 33153489, 3589, 3656, 3926M.HhaI (24)gcgc6, 34, 99, 490, 688, 942, 1040, 1111, 1280, 12881352, 1389, 1655, 1915, 2051, 2405, 2498, 2835, 3206, 33153489, 3589, 3656, 3926M.HinP1I (24)gcgc6, 34, 99, 490, 688, 942, 1040, 1111, 1280, 12881352, 1389, 1655, 1915, 2051, 2405, 2498, 2835, 3206, 33153489, 3589, 3656, 3926M.HpyAVIII (24)gcgc6, 34, 99, 490, 688, 942, 1040, 1111, 1280, 12881352, 1389, 1655, 1915, 2051, 2405, 2498, 2835, 3206, 33153489, 3589, 3656, 3926HpaII (23)ccgg163, 587, 659, 719, 895, 1022, 1193, 1270, 1292, 13201451, 1541, 1608, 1789, 2358, 2392, 2459, 2569, 2811, 32643454, 3480, 3627M.BsuFI (23)ccgg163, 587, 659, 719, 895, 1022, 1193, 1270, 1292, 13201451, 1541, 1608, 1789, 2358, 2392, 2459, 2569, 2811, 32643454, 3480, 3627M.CboI (23)ccgg163, 587, 659, 719, 895, 1022, 1193, 1270, 1292, 13201451, 1541, 1608, 1789, 2358, 2392, 2459, 2569, 2811, 32643454, 3480, 3627M.HapII (23)ccgg163, 587, 659, 719, 895, 1022, 1193, 1270, 1292, 13201451, 1541, 1608, 1789, 2358, 2392, 2459, 2569, 2811, 32643454, 3480, 3627M.HpaII (23)ccgg163, 587, 659, 719, 895, 1022, 1193, 1270, 1292, 13201451, 1541, 1608, 1789, 2358, 2392, 2459, 2569, 2811, 32643454, 3480, 3627M.MjaVI (23)ccgg163, 587, 659, 719, 895, 1022, 1193, 1270, 1292, 13201451, 1541, 1608, 1789, 2358, 2392, 2459, 2569, 2811, 32643454, 3480, 3627M.MspI (23)ccgg163, 587, 659, 719, 895, 1022, 1193, 1270, 1292, 13201451, 1541, 1608, 1789, 2358, 2392, 2459, 2569, 2811, 32643454, 3480, 3627AluI (21)agct58, 122, 217, 239, 440, 683, 908, 961, 987, 10331124, 1393, 1851, 2465, 2565, 2628, 3274, 3531, 3577, 36673893Cac8I (21)gcnngc61, 90, 439, 463, 686, 940, 964, 1015, 1032, 11821368, 1587, 1653, 1687, 1732, 1790, 2389, 3222, 3782, 38193905Hpy188III (21)tcnnga77, 378, 923, 1134, 1307, 1623, 1632, 1715, 1771, 18151905, 1938, 1976, 2106, 2365, 3114, 3188, 3271, 3369, 34673601M.AluI (21)agct58, 122, 217, 239, 440, 683, 908, 961, 987, 10331124, 1393, 1851, 2465, 2565, 2628, 3274, 3531, 3577, 36673893M.EsaBC1I (21)agct58, 122, 217, 239, 440, 683, 908, 961, 987, 10331124, 1393, 1851, 2465, 2565, 2628, 3274, 3531, 3577, 36673893BsrI (19)actgg616, 712, 871, 2324, 2442, 2485, 2924, 3418, 3431, 81356, 382, 913, 994, 1078, 1228, 1429, 2749, 3301HpyCH4V (18)tgca55, 274, 418, 619, 666, 965, 1067, 1179, 1338, 15131527, 2330, 2420, 2613, 2701, 2945, 3223, 3519M.CviRI (18)tgca55, 274, 418, 619, 666, 965, 1067, 1179, 1338, 15131527, 2330, 2420, 2613, 2701, 2945, 3223, 3519M.CviSI (18)tgca55, 274, 418, 619, 666, 965, 1067, 1179, 1338, 15131527, 2330, 2420, 2613, 2701, 2945, 3223, 3519AlwI (17)ggatc605, 851, 1539, 2136, 2600, 2921, 3193, 838, 1102, 11191149, 1460, 1706, 2123, 2903, 3181, 3267HaeIII (17)ggcc41, 310, 358, 460, 613, 709, 1195, 1369, 1760, 17872319, 2399, 2666, 3357, 3791, 3809, 3820M.AlwI (17)ggatc605, 851, 1539, 2136, 2600, 2921, 3193, 838, 1102, 11191149, 1460, 1706, 2123, 2903, 3181, 3267M.AvaVII (17)ggcc41, 310, 358, 460, 613, 709, 1195, 1369, 1760, 17872319, 2399, 2666, 3357, 3791, 3809, 3820M.BspRI (17)ggcc41, 310, 358, 460, 613, 709, 1195, 1369, 1760, 17872319, 2399, 2666, 3357, 3791, 3809, 3820M.BsuRI (17)ggcc41, 310, 358, 460, 613, 709, 1195, 1369, 1760, 17872319, 2399, 2666, 3357, 3791, 3809, 3820M.HaeIII (17)ggcc41, 310, 358, 460, 613, 709, 1195, 1369, 1760, 17872319, 2399, 2666, 3357, 3791, 3809, 3820M.Hpy99VA (17)cctc458, 1146, 1210, 1346, 1703, 2223, 2304, 3662, 3945, 25253, 1044, 1879, 2434, 2640, 3395, 3719M.MnlIA (17)cctc458, 1146, 1210, 1346, 1703, 2223, 2304, 3662, 3945, 25253, 1044, 1879, 2434, 2640, 3395, 3719M.MnlIB (17)cctc458, 1146, 1210, 1346, 1703, 2223, 2304, 3662, 3945, 25253, 1044, 1879, 2434, 2640, 3395, 3719M.MthTI (17)ggcc41, 310, 358, 460, 613, 709, 1195, 1369, 1760, 17872319, 2399, 2666, 3357, 3791, 3809, 3820M.NgoGII (17)ggcc41, 310, 358, 460, 613, 709, 1195, 1369, 1760, 17872319, 2399, 2666, 3357, 3791, 3809, 3820M.NgoPII (17)ggcc41, 310, 358, 460, 613, 709, 1195, 1369, 1760, 17872319, 2399, 2666, 3357, 3791, 3809, 3820M.Phi3TI (17)ggcc41, 310, 358, 460, 613, 709, 1195, 1369, 1760, 17872319, 2399, 2666, 3357, 3791, 3809, 3820M.Rho11sI (17)ggcc41, 310, 358, 460, 613, 709, 1195, 1369, 1760, 17872319, 2399, 2666, 3357, 3791, 3809, 3820M.SPBetaI (17)ggcc41, 310, 358, 460, 613, 709, 1195, 1369, 1760, 17872319, 2399, 2666, 3357, 3791, 3809, 3820M.SPRI (17)ggcc41, 310, 358, 460, 613, 709, 1195, 1369, 1760, 17872319, 2399, 2666, 3357, 3791, 3809, 3820MnlI (17)cctc458, 1146, 1210, 1346, 1703, 2223, 2304, 3662, 3945, 25253, 1044, 1879, 2434, 2640, 3395, 3719BbvI (16)gcagc1249, 1433, 1526, 2329, 3218, 3424, 57, 420, 493, 13501392, 1913, 2695, 3492, 3911, 3929CviAII (16)catg226, 691, 820, 978, 1159, 1504, 1690, 1721, 1747, 21052596, 2606, 2684, 2720, 3113, 3833FatI (16)catg226, 691, 820, 978, 1159, 1504, 1690, 1721, 1747, 21052596, 2606, 2684, 2720, 3113, 3833HpyCH4I (16)catg226, 691, 820, 978, 1159, 1504, 1690, 1721, 1747, 21052596, 2606, 2684, 2720, 3113, 3833HpyF10VI (16)gcnnnnnnngc15, 99, 143, 299, 472, 980, 1280, 1364, 1387, 15261649, 1732, 2398, 3215, 3787, 3901M.BbvI (16)gcagc1249, 1433, 1526, 2329, 3218, 3424, 57, 420, 493, 13501392, 1913, 2695, 3492, 3911, 3929M.BbvSI (16)gcwgc57, 420, 493, 1249, 1350, 1392, 1433, 1526, 1913, 23292695, 3218, 3424, 3492, 3911, 3929M.CviAII (16)catg226, 691, 820, 978, 1159, 1504, 1690, 1721, 1747, 21052596, 2606, 2684, 2720, 3113, 3833M.CviQVII (16)catg226, 691, 820, 978, 1159, 1504, 1690, 1721, 1747, 21052596, 2606, 2684, 2720, 3113, 3833M.CviSII (16)catg226, 691, 820, 978, 1159, 1504, 1690, 1721, 1747, 21052596, 2606, 2684, 2720, 3113, 3833M.EsaBS1I (16)catg226, 691, 820, 978, 1159, 1504, 1690, 1721, 1747, 21052596, 2606, 2684, 2720, 3113, 3833M.EsaDix4I (16)ttaa51, 110, 248, 281, 408, 513, 786, 1959, 2142, 21472161, 2214, 2449, 2488, 2853, 3128M.EsaDix5I (16)ttaa51, 110, 248, 281, 408, 513, 786, 1959, 2142, 21472161, 2214, 2449, 2488, 2853, 3128M.Hpy166IV (16)catg226, 691, 820, 978, 1159, 1504, 1690, 1721, 1747, 21052596, 2606, 2684, 2720, 3113, 3833M.Hpy188II (16)catg226, 691, 820, 978, 1159, 1504, 1690, 1721, 1747, 21052596, 2606, 2684, 2720, 3113, 3833M.Hpy99X (16)catg226, 691, 820, 978, 1159, 1504, 1690, 1721, 1747, 21052596, 2606, 2684, 2720, 3113, 3833M.HpyAI (16)catg226, 691, 820, 978, 1159, 1504, 1690, 1721, 1747, 21052596, 2606, 2684, 2720, 3113, 3833M.HpyI (16)catg226, 691, 820, 978, 1159, 1504, 1690, 1721, 1747, 21052596, 2606, 2684, 2720, 3113, 3833M.MseI (16)ttaa51, 110, 248, 281, 408, 513, 786, 1959, 2142, 21472161, 2214, 2449, 2488, 2853, 3128M.NlaIII (16)catg226, 691, 820, 978, 1159, 1504, 1690, 1721, 1747, 21052596, 2606, 2684, 2720, 3113, 3833MseI (16)ttaa51, 110, 248, 281, 408, 513, 786, 1959, 2142, 21472161, 2214, 2449, 2488, 2853, 3128MwoI (16)gcnnnnnnngc15, 99, 143, 299, 472, 980, 1280, 1364, 1387, 15261649, 1732, 2398, 3215, 3787, 3901NlaIII (16)catg226, 691, 820, 978, 1159, 1504, 1690, 1721, 1747, 21052596, 2606, 2684, 2720, 3113, 3833TseI (16)gcwgc57, 420, 493, 1249, 1350, 1392, 1433, 1526, 1913, 23292695, 3218, 3424, 3492, 3911, 3929Hpy188I (15)tcnga243, 633, 1105, 1128, 1254, 1598, 1951, 2197, 2643, 26542774, 3157, 3291, 3644, 3722M.BflBF4I (15)gcsgc309, 1094, 1197, 1260, 1355, 1520, 1762, 1858, 1899, 26682790, 3019, 3635, 3790, 3908M.CglASI (15)gcsgc309, 1094, 1197, 1260, 1355, 1520, 1762, 1858, 1899, 26682790, 3019, 3635, 3790, 3908M.DsaV (15)ccngg142, 393, 588, 611, 659, 895, 1292, 1452, 1675, 24602811, 3455, 3673, 3686, 3807M.Ecl18kI (15)ccngg142, 393, 588, 611, 659, 895, 1292, 1452, 1675, 24602811, 3455, 3673, 3686, 3807M.Hpy188I (15)tcnga243, 633, 1105, 1128, 1254, 1598, 1951, 2197, 2643, 26542774, 3157, 3291, 3644, 3722M.NlaX (15)ccngg142, 393, 588, 611, 659, 895, 1292, 1452, 1675, 24602811, 3455, 3673, 3686, 3807M.SenPI (15)ccngg142, 393, 588, 611, 659, 895, 1292, 1452, 1675, 24602811, 3455, 3673, 3686, 3807M.Sse9I (15)aatt108, 183, 200, 246, 288, 303, 318, 323, 350, 10261954, 1970, 2145, 2451, 2706M.SsoII (15)ccngg142, 393, 588, 611, 659, 895, 1292, 1452, 1675, 24602811, 3455, 3673, 3686, 3807ScrFI (15)ccngg142, 393, 588, 611, 659, 895, 1292, 1452, 1675, 24602811, 3455, 3673, 3686, 3807StyD4I (15)ccngg142, 393, 588, 611, 659, 895, 1292, 1452, 1675, 24602811, 3455, 3673, 3686, 3807TauI (15)gcsgc309, 1094, 1197, 1260, 1355, 1520, 1762, 1858, 1899, 26682790, 3019, 3635, 3790, 3908Tsp509I (15)aatt108, 183, 200, 246, 288, 303, 318, 323, 350, 10261954, 1970, 2145, 2451, 2706M.TspRI (14)castg94, 355, 617, 713, 968, 1412, 2220, 2325, 2672, 26993148, 3419, 3432, 3938TspRI (14)castg94, 355, 617, 713, 968, 1412, 2220, 2325, 2672, 26993148, 3419, 3432, 3938BscAI (13)gcatc1257, 1512, 1728, 1979, 2735, 3746, 1579, 1643, 1918, 20002023, 2525, 2965M.BspST5I (13)gcatc1257, 1512, 1728, 1979, 2735, 3746, 1579, 1643, 1918, 20002023, 2525, 2965SfaNI (13)gcatc1257, 1512, 1728, 1979, 2735, 3746, 1579, 1643, 1918, 20002023, 2525, 2965BstF5I (12)ggatg426, 2258, 2439, 2726, 560, 598, 677, 898, 1081, 11431600, 1625FokI (12)ggatg426, 2258, 2439, 2726, 560, 598, 677, 898, 1081, 11431600, 1625M.BstF5IA (12)ggatg426, 2258, 2439, 2726, 560, 598, 677, 898, 1081, 11431600, 1625M.BstF5ID (12)ggatg426, 2258, 2439, 2726, 560, 598, 677, 898, 1081, 11431600, 1625M.FokI (12)ggatg426, 2258, 2439, 2726, 560, 598, 677, 898, 1081, 11431600, 1625M.HpyAIIA (12)gaaga841, 1948, 2126, 2881, 2959, 3068, 3184, 3336, 456, 7381638, 1848M.MboIIA (12)gaaga841, 1948, 2126, 2881, 2959, 3068, 3184, 3336, 456, 7381638, 1848M.StsI (12)ggatg426, 2258, 2439, 2726, 560, 598, 677, 898, 1081, 11431600, 1625MaeIII (12)gtnac379, 399, 1410, 1716, 2204, 2535, 2593, 2746, 2934, 32983414, 3477MboII (12)gaaga841, 1948, 2126, 2881, 2959, 3068, 3184, 3336, 456, 7381638, 1848StsI (12)ggatg426, 2258, 2439, 2726, 560, 598, 677, 898, 1081, 11431600, 1625BstYI (11)rgatcy839, 851, 1103, 1461, 1707, 2124, 2136, 2904, 2921, 31823193M.BstYI (11)rgatcy839, 851, 1103, 1461, 1707, 2124, 2136, 2904, 2921, 31823193M.NgoMV (11)ggnncc137, 1041, 1289, 1324, 2057, 2227, 2321, 2362, 2573, 37653804M.XhoII (11)rgatcy839, 851, 1103, 1461, 1707, 2124, 2136, 2904, 2921, 31823193NlaIV (11)ggnncc137, 1041, 1289, 1324, 2057, 2227, 2321, 2362, 2573, 37653804BstUI (10)cgcg33, 307, 761, 1353, 1654, 2052, 2341, 2834, 3207, 3788EaeI (10)yggccr42, 311, 359, 614, 710, 1196, 1370, 1761, 1788, 2667Hpy99I (10)cgwcg362, 375, 594, 1375, 1403, 1697, 1712, 2280, 2543, 3733HpyCH4III (10)acngt485, 884, 1667, 1783, 1891, 2201, 2716, 3331, 3801, 3872M.BepI (10)cgcg33, 307, 761, 1353, 1654, 2052, 2341, 2834, 3207, 3788M.BsuEII (10)cgcg33, 307, 761, 1353, 1654, 2052, 2341, 2834, 3207, 3788M.CfrI (10)yggccr42, 311, 359, 614, 710, 1196, 1370, 1761, 1788, 2667M.EaeI (10)yggccr42, 311, 359, 614, 710, 1196, 1370, 1761, 1788, 2667M.EcoHK31I (10)yggccr42, 311, 359, 614, 710, 1196, 1370, 1761, 1788, 2667M.FnuDII (10)cgcg33, 307, 761, 1353, 1654, 2052, 2341, 2834, 3207, 3788SelI (10)cgcg33, 307, 761, 1353, 1654, 2052, 2341, 2834, 3207, 3788BccI (9)ccatc599, 1082, 1108, 1173, 1601, 1501, 2314, 2438, 2725BsaJI (9)ccnngg142, 393, 589, 611, 895, 1099, 1453, 1722, 3674Bsp1286I (9)gdgchc620, 1232, 1325, 1399, 1589, 1651, 2861, 2946, 3520GdiII (9)cggccr42, 311, 359, 1196, 1761, 1788, 2667, 311, 1196HpyAV (9)ccttc1423, 2396, 3008, 3294, 3604, 477, 1924, 1934, 2633HpyCH4IV (9)acgt373, 501, 622, 860, 1404, 1591, 2503, 2876, 3131M.Hpy99XI (9)acgt373, 501, 622, 860, 1404, 1591, 2503, 2876, 3131M.HpyCH4IV (9)acgt373, 501, 622, 860, 1404, 1591, 2503, 2876, 3131TaiI (9)acgt373, 501, 622, 860, 1404, 1591, 2503, 2876, 3131BslI (8)ccnnnnnnngg39, 733, 1270, 1814, 3355, 3634, 3800, 3818EcoHI (8)ccsgg588, 659, 895, 1292, 1452, 2460, 2811, 3455Hin4I (8)gaynnnnnvtc545, 1467, 1497, 2279, 642, 1467, 2279, 2353HinfI (8)gantc46, 339, 1774, 1908, 2273, 3463, 3859, 3934HphI (8)ggtga844, 1475, 2129, 2356, 2978, 602, 2752, 2993M.BabI (8)gantc46, 339, 1774, 1908, 2273, 3463, 3859, 3934M.BcnIA (8)ccsgg588, 659, 895, 1292, 1452, 2460, 2811, 3455M.BcnIB (8)ccsgg588, 659, 895, 1292, 1452, 2460, 2811, 3455M.CcrMI (8)gantc46, 339, 1774, 1908, 2273, 3463, 3859, 3934M.CviBI (8)gantc46, 339, 1774, 1908, 2273, 3463, 3859, 3934M.CviQVI (8)gantc46, 339, 1774, 1908, 2273, 3463, 3859, 3934M.Eco1831I (8)ccsgg588, 659, 895, 1292, 1452, 2460, 2811, 3455M.EcoHI (8)ccsgg588, 659, 895, 1292, 1452, 2460, 2811, 3455M.HhaII (8)gantc46, 339, 1774, 1908, 2273, 3463, 3859, 3934M.HinfI (8)gantc46, 339, 1774, 1908, 2273, 3463, 3859, 3934M.HphIA (8)ggtga844, 1475, 2129, 2356, 2978, 602, 2752, 2993M.HphIB (8)ggtga844, 1475, 2129, 2356, 2978, 602, 2752, 2993M.Hpy99IX (8)gantc46, 339, 1774, 1908, 2273, 3463, 3859, 3934M.HpyAIV (8)gantc46, 339, 1774, 1908, 2273, 3463, 3859, 3934M.NgoBVIIIA (8)ggtga844, 1475, 2129, 2356, 2978, 602, 2752, 2993M.NgoHVIII (8)ggtga844, 1475, 2129, 2356, 2978, 602, 2752, 2993M.SscL1I (8)gantc46, 339, 1774, 1908, 2273, 3463, 3859, 3934M.StyLTI (8)cagag1046, 1127, 1253, 3375, 3401, 3721, 567, 3312NciI (8)ccsgg588, 659, 895, 1292, 1452, 2460, 2811, 3455SspD5I (8)ggtga844, 1475, 2129, 2356, 2978, 602, 2752, 2993TspDTI (8)atgaa48, 1776, 2256, 2559, 679, 900, 1333, 2154BsiEI (7)cgrycg311, 471, 1196, 2646, 2795, 3497, 3921BstNI (7)ccwgg142, 393, 611, 1675, 3673, 3686, 3807CjeI (7)ccannnnnngt3252, 3807, 445, 622, 3153, 3253, 3331DdeI (7)ctnag242, 296, 753, 2235, 2775, 3150, 3559EsaBC3I (7)tcga1401, 1557, 1581, 1617, 1779, 2928, 3734Hpy8I (7)gtnnac520, 620, 650, 1075, 2188, 2946, 3520M.BstNI (7)ccwgg142, 393, 611, 1675, 3673, 3686, 3807M.CviBIII (7)tcga1401, 1557, 1581, 1617, 1779, 2928, 3734M.CviQIII (7)tcga1401, 1557, 1581, 1617, 1779, 2928, 3734M.CviSIII (7)tcga1401, 1557, 1581, 1617, 1779, 2928, 3734M.DdeI (7)ctnag242, 296, 753, 2235, 2775, 3150, 3559M.EcoKDcm (7)ccwgg142, 393, 611, 1675, 3673, 3686, 3807M.EcoRII (7)ccwgg142, 393, 611, 1675, 3673, 3686, 3807M.EsaBC3I (7)tcga1401, 1557, 1581, 1617, 1779, 2928, 3734M.EsaDix3I (7)tcga1401, 1557, 1581, 1617, 1779, 2928, 3734M.Hpy8I (7)gtnnac520, 620, 650, 1075, 2188, 2946, 3520M.HpyAIX (7)gtnnac520, 620, 650, 1075, 2188, 2946, 3520M.HpyAX (7)tcga1401, 1557, 1581, 1617, 1779, 2928, 3734M.MvaI (7)ccwgg142, 393, 611, 1675, 3673, 3686, 3807M.Phi3TII (7)tcga1401, 1557, 1581, 1617, 1779, 2928, 3734M.TaqI (7)tcga1401, 1557, 1581, 1617, 1779, 2928, 3734M.TthHB8I (7)tcga1401, 1557, 1581, 1617, 1779, 2928, 3734MspA1I (7)cmgckg59, 441, 1034, 1394, 2912, 3249, 3494PspGI (7)ccwgg142, 393, 611, 1675, 3673, 3686, 3807TaqI (7)tcga1401, 1557, 1581, 1617, 1779, 2928, 3734BceAI (6)acggc360, 541, 1263, 3348, 506, 1699BcefI (6)acggc360, 541, 1263, 3348, 506, 1699BfaI (6)ctag267, 847, 989, 2132, 2467, 3339BseMII (6)ctcag2775, 3150, 3559, 242, 753, 2235BsiHKAI (6)gwgcwc620, 1399, 1589, 2861, 2946, 3520BspCNI (6)ctcag2775, 3150, 3559, 242, 753, 2235CdiI (6)catcg1727, 2930, 1575, 1778, 1919, 2526CjePI (6)ccannnnnnntc685, 2192, 2664, 3301, 623, 3154FmuI (6)ggncc461, 1805, 2320, 2399, 2416, 2638HaeI (6)wggccw614, 710, 1370, 3358, 3810, 3821M.Cfr13I (6)ggncc461, 1805, 2320, 2399, 2416, 2638M.EcoPI (6)agacc722, 1611, 2195, 1309, 2346, 3159M.MthZI (6)ctag267, 847, 989, 2132, 2467, 3339M.PspPI (6)ggncc461, 1805, 2320, 2399, 2416, 2638M.Sau96I (6)ggncc461, 1805, 2320, 2399, 2416, 2638Sau96I (6)ggncc461, 1805, 2320, 2399, 2416, 2638UnbI (6)ggncc461, 1805, 2320, 2399, 2416, 2638BanI (5)ggyrcc137, 1041, 1289, 1324, 2227Bme1580I (5)gkgcmc620, 1232, 1325, 2946, 3520BmrI (5)actggg2324, 383, 914, 995, 1229BpuEI (5)cttgag1681, 2899, 3466, 3189, 3728BsaWI (5)wccggw1023, 1321, 2570, 3481, 3628BsmAI (5)gtctc1137, 2345, 642, 723, 3110Eco57MI (5)ctgrag2364, 2961, 3307, 1415, 1847M.Alw26I (5)gtctc1137, 2345, 642, 723, 3110M.BfiIA (5)actggg2324, 383, 914, 995, 1229M.BfiIB (5)actggg2324, 383, 914, 995, 1229M.HgiCI (5)ggyrcc137, 1041, 1289, 1324, 2227M.Hpy99II (5)gtsac379, 1410, 1716, 2535, 2746M.Tsp45I (5)gtsac379, 1410, 1716, 2535, 2746MslI (5)caynnnnrtg706, 1727, 2531, 2690, 3049SfcI (5)ctryag275, 333, 2520, 3378, 3569SimI (5)gggtc892, 1718, 2347, 3160, 3641SmlI (5)ctyrag1681, 2899, 3189, 3466, 3728Tsp45I (5)gtsac379, 1410, 1716, 2535, 2746Tth111II (5)caarca554, 1735, 3221, 3260, 3227AcuI (4)ctgaag2961, 3307, 1415, 1847BspMI (4)acctgc277, 957, 1186, 1549BsrBI (4)ccgctc191, 1901, 2102, 3903BsrFI (4)rccggy720, 1609, 1790, 2359BtsI (4)gcagtg2700, 94, 968, 2672Csp6I (4)gtac503, 563, 1593, 2756DraI (4)tttaaa282, 2143, 2162, 2854EarI (4)ctcttc457, 1639, 1849, 3068EciI (4)ggcgga2432, 1803, 3616, 3762FauI (4)cccgc89, 1419, 32, 463HaeII (4)rgcgcy7, 1041, 1289, 3590LpnI (4)rgcgcy7, 1041, 1289, 3590M.AvaIX (4)rccggy720, 1609, 1790, 2359M.Cfr10I (4)rccggy720, 1609, 1790, 2359M.Cfr6I (4)cagctg59, 441, 1034, 1394M.CviQI (4)gtac503, 563, 1593, 2756M.CviRII (4)gtac503, 563, 1593, 2756M.DraI (4)tttaaa282, 2143, 2162, 2854M.Eco57IA (4)ctgaag2961, 3307, 1415, 1847M.Eco57IB (4)ctgaag2961, 3307, 1415, 1847M.EcoP15I (4)cagcag628, 3217, 3426, 3310M.MlyI (4)gastc339, 2273, 3463, 3934M.PvuII (4)cagctg59, 441, 1034, 1394M.RsaI (4)gtac503, 563, 1593, 2756M.TfiI (4)gawtc46, 1774, 1908, 3859MlyI (4)gagtc2273, 3463, 339, 3934PleI (4)gagtc2273, 3463, 339, 3934PvuII (4)cagctg59, 441, 1034, 1394RsaI (4)gtac503, 563, 1593, 2756TaqII (4)gaccga2637, 1311, 2796, 3922TfiI (4)gawtc46, 1774, 1908, 3859TspGWI (4)acgga726, 2728, 596, 3045ApaLI (3)gtgcac620, 2946, 3520AseI (3)attaat52, 111, 2450AvaII (3)ggwcc1805, 2416, 2638BciVI (3)gtatcc1498, 2098, 3625BsaAI (3)yacgtr502, 861, 1592BsaHI (3)grcgyc1041, 1289, 2815BseYI (3)cccagc1036, 1794, 3530BsrDI (3)gcaatg2515, 1516, 2333BssSI (3)cacgag1882, 2943, 3661FspI (3)tgcgca491, 1390, 2499HgaI (3)gacgc2815, 3154, 3732M.BsaAI (3)yacgtr502, 861, 1592M.HgaIA (3)gacgc2815, 3154, 3732M.HgaIB (3)gacgc2815, 3154, 3732M.SinI (3)ggwcc1805, 2416, 2638M.VspI (3)attaat52, 111, 2450MscI (3)tggcca614, 710, 1370Psp03I (3)ggwcc1805, 2416, 2638VpaK11AI (3)ggwcc1805, 2416, 2638AceIII (2)cagctc3894, 2566AclI (2)aacgtt2504, 2877AlwNI (2)cagnnnctg912, 3423BbeI (2)ggcgcc1041, 1289BglI (2)gccnnnnnggc299, 2398BsaBI (2)gatnnnnatc604, 1149BsaI (2)ggtctc2346, 723BsmFI (2)gggac264, 1426Bsp24I (2)gacnnnnnntgg623, 3154BspHI (2)tcatga2106, 3114DrdI (2)gacnnnnnngtc1317, 3732EagI (2)cggccg311, 1196HaeIV (2)gaynnnnnrtc1467, 2279KasI (2)ggcgcc1041, 1289M.AclI (2)aacgtt2504, 2877M.BspLU11IIIA (2)gggac264, 1426M.BspLU11IIIC (2)gggac264, 1426M.CfrBI (2)ccwwgg1099, 1722M.HaeIV (2)gaynnnnnrtc1467, 2279M.MmeIA (2)tccrac3460, 3644M.MmeIB (2)tccrac3460, 3644M.NgoBIX (2)gtannnnnctc3567, 2940M.NspHI (2)rcatgy1691, 3834M.NspI (2)rcatgy1691, 3834M.PhiMx8I (2)ctssag275, 2364M.XmaIII (2)cggccg311, 1196MmeI (2)tccrac3460, 3644NarI (2)ggcgcc1041, 1289NspI (2)rcatgy1691, 3834PpiI (2)gaacnnnnnctc2926, 3143PvuI (2)cgatcg471, 2646SapI (2)gctcttc1640, 1850SfoI (2)ggcgcc1041, 1289StyI (2)ccwwgg1099, 1722TatI (2)wgtacw564, 2757AflIII (1)acrygt3834AhdI (1)gacnnnnngtc2279ApoI (1)raatty319BanII (1)grgcyc1651BcgI (1)cgannnnnntgc2801BclI (1)tgatca1131BmgBI (1)cacgtc623BpmI (1)ctggag2364BsaXI (1)acnnnnnctcc28BsiWI (1)cgtacg504BsrGI (1)tgtaca564BstXI (1)ccannnnnntgg707Bsu36I (1)cctnagg297BtgI (1)ccrygg1722FalI (1)aagnnnnnctt1612M.AflIII (1)acrygt3834M.BanII (1)grgcyc1651M.BcgI (1)cgannnnnntgc2801M.BclI (1)tgatca1131M.BmeTI (1)tgatca1131M.BsuBI (1)ctgcag275M.EcoKI (1)aacnnnnnngtgc2944M.EcoR124II (1)gaannnnnnnrtcg1580M.NgoMIV (1)gccggc1790M.NmeSI (1)agtact2757M.PstI (1)ctgcag275M.SauLPI (1)gccggc1790M.ScaI (1)agtact2757M.SnaBI (1)tacgta502M.StyLTIII (1)gagnnnnnnrtayg2786M.StySPI (1)aacnnnnnngtrc2944M.TaeII (1)tgatca1131M.Tth111I (1)gacnnngtc1408M.XcmI (1)ccannnnnnnnntgg1108NaeI (1)gccggc1790NcoI (1)ccatgg1722NgoMIV (1)gccggc1790NotI (1)gcggccgc312PciI (1)acatgt3834PmeI (1)gtttaaac283PstI (1)ctgcag275RsrII (1)cggwccg1806SbfI (1)cctgcagg276ScaI (1)agtact2757SnaBI (1)tacgta502SpeI (1)actagt268SphI (1)gcatgc1691SspI (1)aatatt3081Tth111I (1)gacnnngtc1408XcmI (1)ccannnnnnnnntgg1108XmnI (1)gaannnnttc2878 Non-Cutters AarI, AatII, Acc65I, AccI, AfeI, AflII, AgeI, AleI, AloI, ApaBI ApaI, AscI, AsiSI, AvaI, AvrII, BaeI, BamHI, Bbr7I, BbsI, BbvCI BfrBI, BglII, BlpI, BmtI, BplI, Bpu10I, BseRI, BsgI, BsmBI, BsmI BspEI, BssHII, BstAPI, BstBI, BstEII, BstZ17I, ClaI, DraIII, EcoICRI, EcoNI EcoO109I, EcoRI, EcoRV, FseI, FspAI, HincII, HindIII, HpaI, KpnI, M.AccI M.AloI, M.ApaI, M.AquI, M.BaeI, M.BbvCIA, M.BbvCIB, M.BglII, M.BnaI, M.BseCI, M.Bsp106I M.BstI, M.BstVI, M.Bsu15I, M.Cfr9I, M.ClaI, M.DraIII, M.EcaI, M.EcoAI, M.EcoBI, M.EcoO109I M.EcoR124I, M.EcoRI, M.EcoRV, M.Esp3I, M.HincII, M.HindII, M.HindIII, M.HpaI, M.KpnAI, M.KpnBI M.KpnI, M.MunI, M.NgoAV, M.PaeR7I, M.PhiBssHII, M.PshAI, M.Rrh4273I, M.RsrI, M.SacI, M.SalI M.Sau3239I, M.SmaI, M.SsoI, M.XbaI, M.XcyI, M.XhoI, MfeI, MluI, NdeI, NheI Nli3877I, NruI, NsiI, PacI, PflMI, PfoI, PmlI, Ppu10I, PpuMI, PshAI PsiI, PspOMI, PsrI, PssI, RleAI, SacI, SacII, SalI, SanDI, SciI SexAI, SfiI, SgrAI, SmaI, SrfI, Sse232I, Sse8647I, StuI, SwaI, UthSI XbaI, XhoI, XmaI, ZraI       $& E F G L P Q 2:TVȼȭȼȥi/h{h{5B*CJOJQJ^JaJo(ph!h{0J5CJOJQJ^JaJ$h{0J5CJOJQJ^JaJo(h:hro(hDvh:fHq & hovo( hDvh: h:h: ho( h go( hWo(hhShqX*hIB*CJKHOJQJ^JaJo(ph!%&L8A]^ $7$8$H$a$gdr$a$gd{gdrgdgdS } gdqXgdqX$ @1$7$8$H$^`@a$gdI6¯{{jV?,h4hrCJKHOJQJ^JaJmH sH 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