ࡱ>  {}rstuvwxyzg R bjbjVV=r<r< ,,,,,@@@8x$@GY@,@,@,@,@,t-t-t-XXXXXXX$[]LX9,z.t-t-z.z.X,,@,@,4YJVJVJVz.&,@,,@,XJVz.XJVJVJV@,̕CUJVXY0GYJV^UR^JVJV^,^V0t--JV- -t-t-t-XX V@t-t-t-GYz.z.z.z.^t-t-t-t-t-t-t-t-t-, :  pEASY-Blunt Zero Complete sequence AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCAGAATTAACCCTCACTAAAGGGACTAGTCCTGCAGGTTTAAACGAATTGGCCCTTAAGGGCCAATTCGCGGCCGCTAAATTCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTATACGTACGGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCGGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTCACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGCTCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTATTAACGCTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG M13F5'-GTAAAACGACGGCCAGT-3' vU_SGJ101-01 M13R5'-CAGGAAACAGCTATGAC-3' vU_SGJ101-11 LacZa-ccdB fragment: bases 217-808 M13 reverse priming site: bases 205-221 T7 promoter/priming site: bases 326-345 M13 Forward priming site: bases 353-368 Kanamycin resistance ORF: bases 1157-1951 Ampicillin resistance ORF: bases 2201-3061 pUC origin: bases 3159-3832 pEASY-Blunt Zero Cutters M.CviQII (352)rar25, 46, 72, 123, 127, 145, 152, 159, 166, 201240, 253, 279, 294, 305, 320, 325, 352, 366, 407416, 432, 477, 512, 519, 554, 643, 653, 724, 752797, 841, 854, 963, 974, 1002, 1045, 1086, 1090, 11261154, 1189, 1200, 1217, 1252, 1268, 1296, 1300, 1383, 14001463, 1473, 1478, 1482, 1535, 1556, 1596, 1613, 1652, 16701679, 1709, 1735, 1765, 1775, 1853, 1876, 1880, 1886, 19021909, 1924, 1934, 1946, 1965, 1972, 1987, 1991, 2013, 20352065, 2069, 2073, 2087, 2103, 2126, 2139, 2159, 2191, 22132234, 2250, 2255, 2274, 2309, 2354, 2362, 2369, 2425, 24352480, 2495, 2540, 2547, 2557, 2562, 2567, 2573, 2630, 26342641, 2682, 2708, 2712, 2738, 2759, 2772, 2804, 2851, 28622879, 2895, 2906, 2927, 2941, 2959, 2968, 2974, 2986, 30603066, 3072, 3076, 3094, 3111, 3127, 3138, 3144, 3184, 31963200, 3221, 3256, 3260, 3283, 3287, 3305, 3334, 3365, 33743396, 3400, 3450, 3464, 3515, 3533, 3597, 3669, 3693, 37113720, 3726, 3738, 3749, 3794, 3804, 3812, 3823, 3829, 38373841, 3860, 3884, 3904, 18, 83, 96, 116, 181, 188211, 237, 244, 259, 282, 286, 297, 317, 338, 386455, 515, 530, 534, 538, 568, 638, 650, 680, 737741, 764, 776, 804, 810, 823, 832, 865, 880, 901928, 932, 940, 946, 951, 1015, 1019, 1025, 1052, 10601070, 1075, 1099, 1123, 1134, 1146, 1166, 1170, 1208, 13071330, 1334, 1341, 1346, 1415, 1422, 1445, 1490, 1494, 15661570, 1583, 1587, 1604, 1637, 1664, 1682, 1687, 1703, 17411754, 1847, 1862, 1943, 1953, 2044, 2056, 2080, 2090, 21082117, 2144, 2149, 2155, 2163, 2173, 2184, 2188, 2223, 23042344, 2381, 2395, 2403, 2410, 2463, 2470, 2474, 2585, 25902616, 2620, 2656, 2661, 2732, 2754, 2766, 2776, 2798, 28482855, 2872, 2890, 2900, 2918, 2995, 2999, 3007, 3013, 30233027, 3031, 3035, 3047, 3055, 3088, 3120, 3135, 3151, 31683177, 3190, 3226, 3230, 3234, 3271, 3293, 3313, 3326, 33693441, 3467, 3486, 3505, 3537, 3541, 3555, 3560, 3576, 35833587, 3603, 3608, 3612, 3632, 3646, 3652, 3662, 3666, 36793729, 3764, 3773, 3888, 3892, 3916, 3924, 3933, 3941, 39453949, 3954M.CviPI (297)gc4, 15, 22, 32, 40, 54, 57, 61, 86, 9097, 104, 121, 134, 143, 157, 164, 189, 216, 233238, 273, 290, 300, 307, 310, 313, 327, 358, 395413, 418, 421, 436, 440, 444, 452, 460, 464, 473489, 494, 507, 539, 585, 591, 613, 619, 627, 666683, 687, 709, 717, 745, 755, 761, 781, 818, 868876, 887, 908, 916, 937, 941, 954, 961, 965, 972981, 987, 997, 1012, 1016, 1029, 1033, 1039, 1062, 10671084, 1092, 1095, 1110, 1124, 1157, 1179, 1183, 1195, 11981212, 1220, 1231, 1247, 1250, 1258, 1261, 1272, 1279, 12871324, 1338, 1348, 1351, 1356, 1365, 1369, 1379, 1388, 13931398, 1417, 1431, 1434, 1442, 1449, 1455, 1480, 1486, 15071513, 1518, 1521, 1524, 1527, 1533, 1543, 1550, 1567, 15771584, 1588, 1606, 1641, 1650, 1654, 1660, 1672, 1677, 16841688, 1692, 1700, 1724, 1729, 1733, 1737, 1760, 1763, 17871791, 1801, 1808, 1822, 1828, 1843, 1851, 1856, 1859, 18671874, 1884, 1897, 1900, 1911, 1914, 1921, 1931, 1963, 19801998, 2006, 2021, 2031, 2050, 2101, 2211, 2225, 2237, 22672307, 2319, 2327, 2330, 2341, 2352, 2360, 2375, 2386, 23902399, 2404, 2420, 2432, 2457, 2465, 2482, 2497, 2510, 25162523, 2538, 2555, 2565, 2587, 2613, 2621, 2628, 2666, 26692693, 2696, 2701, 2722, 2736, 2788, 2791, 2802, 2808, 28132834, 2844, 2860, 2887, 2911, 2945, 2963, 2982, 2996, 30103017, 3020, 3038, 3090, 3152, 3205, 3216, 3219, 3223, 32413248, 3262, 3274, 3303, 3309, 3314, 3346, 3357, 3380, 33853398, 3403, 3422, 3425, 3428, 3435, 3488, 3493, 3501, 35193526, 3531, 3538, 3571, 3577, 3588, 3595, 3614, 3633, 36363655, 3667, 3683, 3714, 3730, 3747, 3760, 3765, 3779, 37833788, 3791, 3809, 3816, 3820, 3827, 3845, 3881, 3893, 39023906, 3909, 3912, 3925, 3930, 3942, 3950M.SssI (252)cg5, 14, 21, 31, 35, 42, 64, 76, 87, 98103, 162, 167, 190, 232, 284, 306, 312, 326, 341360, 363, 373, 376, 396, 412, 435, 445, 453, 463468, 472, 490, 501, 505, 541, 547, 584, 587, 592595, 622, 646, 659, 672, 688, 702, 719, 726, 732760, 780, 860, 875, 883, 895, 936, 942, 982, 9981017, 1022, 1040, 1094, 1111, 1151, 1156, 1182, 1193, 11971218, 1245, 1260, 1263, 1270, 1280, 1288, 1292, 1311, 13201344, 1352, 1361, 1373, 1376, 1380, 1389, 1401, 1404, 14181443, 1451, 1488, 1519, 1522, 1532, 1541, 1557, 1568, 15761581, 1585, 1591, 1597, 1608, 1617, 1635, 1653, 1662, 16711685, 1695, 1698, 1701, 1710, 1713, 1725, 1739, 1762, 17791789, 1802, 1807, 1823, 1834, 1857, 1860, 1873, 1881, 18901896, 1899, 1905, 1910, 1915, 1920, 1930, 1941, 1962, 19811997, 2007, 2020, 2039, 2051, 2100, 2238, 2252, 2278, 22812295, 2300, 2340, 2351, 2358, 2392, 2401, 2405, 2431, 24592481, 2498, 2503, 2527, 2537, 2541, 2544, 2569, 2579, 25882622, 2635, 2643, 2647, 2668, 2728, 2789, 2792, 2796, 28112814, 2822, 2833, 2876, 2883, 2888, 2910, 2928, 2942, 29833019, 3039, 3044, 3131, 3141, 3153, 3163, 3206, 3240, 32493264, 3291, 3315, 3348, 3384, 3397, 3436, 3444, 3454, 34703480, 3489, 3494, 3498, 3507, 3516, 3539, 3543, 3553, 35723589, 3594, 3601, 3615, 3627, 3634, 3643, 3656, 3660, 36823706, 3713, 3731, 3734, 3750, 3761, 3778, 3787, 3800, 38463872, 3880, 3901, 3905, 3908, 3914, 3918, 3922, 3926, 39313943, 3951M.CviAIV (242)rgcb7, 23, 41, 58, 122, 144, 165, 217, 234, 239311, 328, 359, 414, 437, 461, 474, 495, 540, 586614, 684, 710, 746, 756, 782, 869, 909, 917, 962973, 988, 1034, 1042, 1063, 1085, 1096, 1125, 1196, 12131221, 1248, 1262, 1273, 1290, 1357, 1366, 1394, 1432, 15081525, 1534, 1544, 1607, 1651, 1657, 1673, 1678, 1761, 17881809, 1829, 1852, 1868, 1875, 1898, 1922, 1932, 1964, 21022308, 2320, 2353, 2361, 2387, 2400, 2433, 2466, 2483, 25392556, 2566, 2629, 2667, 2837, 2912, 3242, 3275, 3304, 33473358, 3386, 3399, 3423, 3502, 3527, 3532, 3578, 3591, 35963668, 3684, 3715, 3766, 3780, 3792, 3810, 3821, 3882, 38943903, 3913, 5, 41, 58, 87, 98, 122, 144, 165190, 217, 239, 311, 359, 396, 441, 453, 461, 495540, 592, 614, 684, 710, 746, 756, 869, 909, 917942, 962, 973, 982, 988, 998, 1017, 1034, 1040, 10631085, 1111, 1125, 1196, 1213, 1221, 1248, 1273, 1280, 12881352, 1357, 1366, 1380, 1394, 1418, 1432, 1443, 1508, 15221544, 1568, 1585, 1607, 1651, 1661, 1678, 1685, 1701, 17251761, 1788, 1802, 1823, 1829, 1852, 1857, 1868, 1915, 22382308, 2320, 2361, 2387, 2400, 2405, 2466, 2556, 2566, 25882622, 2629, 2667, 2792, 2814, 2888, 2983, 3039, 3153, 32493275, 3304, 3315, 3347, 3358, 3423, 3436, 3489, 3494, 35023527, 3532, 3539, 3572, 3578, 3589, 3615, 3634, 3656, 36683731, 3761, 3766, 3792, 3810, 3821, 3894, 3913, 3926, 39313943, 3951M.CviJI (242)rgcb7, 23, 41, 58, 122, 144, 165, 217, 234, 239311, 328, 359, 414, 437, 461, 474, 495, 540, 586614, 684, 710, 746, 756, 782, 869, 909, 917, 962973, 988, 1034, 1042, 1063, 1085, 1096, 1125, 1196, 12131221, 1248, 1262, 1273, 1290, 1357, 1366, 1394, 1432, 15081525, 1534, 1544, 1607, 1651, 1657, 1673, 1678, 1761, 17881809, 1829, 1852, 1868, 1875, 1898, 1922, 1932, 1964, 21022308, 2320, 2353, 2361, 2387, 2400, 2433, 2466, 2483, 25392556, 2566, 2629, 2667, 2837, 2912, 3242, 3275, 3304, 33473358, 3386, 3399, 3423, 3502, 3527, 3532, 3578, 3591, 35963668, 3684, 3715, 3766, 3780, 3792, 3810, 3821, 3882, 38943903, 3913, 5, 41, 58, 87, 98, 122, 144, 165190, 217, 239, 311, 359, 396, 441, 453, 461, 495540, 592, 614, 684, 710, 746, 756, 869, 909, 917942, 962, 973, 982, 988, 998, 1017, 1034, 1040, 10631085, 1111, 1125, 1196, 1213, 1221, 1248, 1273, 1280, 12881352, 1357, 1366, 1380, 1394, 1418, 1432, 1443, 1508, 15221544, 1568, 1585, 1607, 1651, 1661, 1678, 1685, 1701, 17251761, 1788, 1802, 1823, 1829, 1852, 1857, 1868, 1915, 22382308, 2320, 2361, 2387, 2400, 2405, 2466, 2556, 2566, 25882622, 2629, 2667, 2792, 2814, 2888, 2983, 3039, 3153, 32493275, 3304, 3315, 3347, 3358, 3423, 3436, 3489, 3494, 35023527, 3532, 3539, 3572, 3578, 3589, 3615, 3634, 3656, 36683731, 3761, 3766, 3792, 3810, 3821, 3894, 3913, 3926, 39313943, 3951M.HindI (103)cac94, 114, 176, 186, 336, 378, 557, 570, 602, 618648, 662, 714, 743, 886, 969, 1205, 1265, 1323, 13631397, 1448, 1715, 1751, 1785, 1797, 1836, 1883, 2005, 20292049, 2221, 2283, 2326, 2413, 2529, 2612, 2673, 2745, 27522783, 2859, 2944, 2993, 3133, 3252, 3342, 3420, 3433, 34463518, 3574, 3743, 3864, 3890, 3939, 63, 125, 136, 150204, 255, 354, 423, 466, 523, 583, 621, 700, 758844, 859, 1181, 1233, 1372, 1411, 1475, 1562, 1590, 20162033, 2129, 2227, 2350, 2356, 2536, 2680, 2698, 2839, 29392947, 2978, 3043, 3147, 3238, 3360, 3382, 3521, 3551, 35933659, 3717, 3831CviJI (75)rgcy41, 58, 122, 144, 165, 217, 239, 311, 359, 441461, 495, 540, 614, 684, 710, 746, 756, 869, 909917, 962, 973, 988, 1034, 1063, 1085, 1125, 1196, 12131221, 1248, 1273, 1357, 1366, 1394, 1432, 1508, 1525, 15441607, 1651, 1661, 1678, 1761, 1788, 1829, 1852, 1868, 23082320, 2361, 2387, 2400, 2466, 2556, 2566, 2629, 2667, 32753304, 3347, 3358, 3423, 3502, 3527, 3532, 3578, 3668, 37663792, 3810, 3821, 3894, 3913M.CviTI (75)rgcy41, 58, 122, 144, 165, 217, 239, 311, 359, 441461, 495, 540, 614, 684, 710, 746, 756, 869, 909917, 962, 973, 988, 1034, 1063, 1085, 1125, 1196, 12131221, 1248, 1273, 1357, 1366, 1394, 1432, 1508, 1525, 15441607, 1651, 1661, 1678, 1761, 1788, 1829, 1852, 1868, 23082320, 2361, 2387, 2400, 2466, 2556, 2566, 2629, 2667, 32753304, 3347, 3358, 3423, 3502, 3527, 3532, 3578, 3668, 37663792, 3810, 3821, 3894, 3913M.NgoMXV (57)gcchr40, 61, 90, 134, 143, 358, 395, 444, 613, 687818, 916, 972, 997, 1231, 1369, 1379, 1431, 1442, 16771828, 1856, 1867, 2319, 2523, 2666, 3303, 3357, 3501, 38093820, 9, 236, 439, 463, 497, 616, 712, 871, 10321098, 1292, 1553, 1659, 1675, 1695, 1732, 2270, 2389, 24852811, 3388, 3431, 3529, 3686, 3768, 3782AciI (47)ccgc88, 191, 309, 999, 1355, 1419, 1520, 1803, 1858, 19822008, 2054, 2623, 2790, 3250, 3495, 3616, 3635, 3762, 37903952, 22, 32, 313, 464, 761, 876, 1095, 1198, 12611763, 1808, 1874, 1900, 2021, 2101, 2341, 2432, 2669, 28342911, 3020, 3241, 3385, 3881, 3902, 3909Sth132I (30)cccg89, 589, 674, 734, 1378, 1420, 1453, 2041, 2302, 24612824, 2885, 3456, 3500, 3509, 3708, 31, 76, 463, 646659, 895, 1292, 1695, 1834, 1905, 2278, 2811, 3163, 3601BthCI (29)gcngc57, 310, 421, 494, 1095, 1198, 1250, 1261, 1351, 13931434, 1521, 1527, 1763, 1859, 1900, 1914, 2330, 2669, 26962791, 3020, 3219, 3425, 3493, 3636, 3791, 3909, 3930Fnu4HI (29)gcngc57, 310, 421, 494, 1095, 1198, 1250, 1261, 1351, 13931434, 1521, 1527, 1763, 1859, 1900, 1914, 2330, 2669, 26962791, 3020, 3219, 3425, 3493, 3636, 3791, 3909, 3930BstKTI (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268ChaI (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268DpnI (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.AacDam (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.AlaK2I (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.AvaV (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.AvaVI (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.BspKT6I (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.CviAI (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.DpnIIA (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.DpnIIB (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.Eco67Dam (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.EcoKDam (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.EcoP1Dam (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.EcoT1Dam (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.EcoT2Dam (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.EcoT4Dam (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.EsaLHCI (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.HindDam (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.HinHP1Dam (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.Hpy99VI (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.HpyAIII (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.LlaDCHIA (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.LlaDCHIB (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.LlaKR2I (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.MboIA (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.MboIB (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.PgiI (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.PhiCh1I (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.Sau3AI (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.SmaII (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.Sth368I (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.Sty1344Dam (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.StyDam (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268M.TpaI (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268MboI (27)gatc471, 605, 750, 839, 851, 1103, 1120, 1131, 1150, 14611539, 1620, 1629, 1707, 2124, 2136, 2241, 2582, 2600, 26462904, 2921, 2957, 3174, 3182, 3193, 3268HhaI (24)gcgc6, 34, 99, 491, 689, 943, 1041, 1112, 1281, 12891353, 1390, 1656, 1916, 2052, 2406, 2499, 2836, 3207, 33163490, 3590, 3657, 3927HinP1I (24)gcgc6, 34, 99, 491, 689, 943, 1041, 1112, 1281, 12891353, 1390, 1656, 1916, 2052, 2406, 2499, 2836, 3207, 33163490, 3590, 3657, 3927M.HhaI (24)gcgc6, 34, 99, 491, 689, 943, 1041, 1112, 1281, 12891353, 1390, 1656, 1916, 2052, 2406, 2499, 2836, 3207, 33163490, 3590, 3657, 3927M.HinP1I (24)gcgc6, 34, 99, 491, 689, 943, 1041, 1112, 1281, 12891353, 1390, 1656, 1916, 2052, 2406, 2499, 2836, 3207, 33163490, 3590, 3657, 3927M.HpyAVIII (24)gcgc6, 34, 99, 491, 689, 943, 1041, 1112, 1281, 12891353, 1390, 1656, 1916, 2052, 2406, 2499, 2836, 3207, 33163490, 3590, 3657, 3927HpaII (23)ccgg163, 588, 660, 720, 896, 1023, 1194, 1271, 1293, 13211452, 1542, 1609, 1790, 2359, 2393, 2460, 2570, 2812, 32653455, 3481, 3628M.BsuFI (23)ccgg163, 588, 660, 720, 896, 1023, 1194, 1271, 1293, 13211452, 1542, 1609, 1790, 2359, 2393, 2460, 2570, 2812, 32653455, 3481, 3628M.CboI (23)ccgg163, 588, 660, 720, 896, 1023, 1194, 1271, 1293, 13211452, 1542, 1609, 1790, 2359, 2393, 2460, 2570, 2812, 32653455, 3481, 3628M.HapII (23)ccgg163, 588, 660, 720, 896, 1023, 1194, 1271, 1293, 13211452, 1542, 1609, 1790, 2359, 2393, 2460, 2570, 2812, 32653455, 3481, 3628M.HpaII (23)ccgg163, 588, 660, 720, 896, 1023, 1194, 1271, 1293, 13211452, 1542, 1609, 1790, 2359, 2393, 2460, 2570, 2812, 32653455, 3481, 3628M.MjaVI (23)ccgg163, 588, 660, 720, 896, 1023, 1194, 1271, 1293, 13211452, 1542, 1609, 1790, 2359, 2393, 2460, 2570, 2812, 32653455, 3481, 3628M.MspI (23)ccgg163, 588, 660, 720, 896, 1023, 1194, 1271, 1293, 13211452, 1542, 1609, 1790, 2359, 2393, 2460, 2570, 2812, 32653455, 3481, 3628AluI (21)agct58, 122, 217, 239, 441, 684, 909, 962, 988, 10341125, 1394, 1852, 2466, 2566, 2629, 3275, 3532, 3578, 36683894Cac8I (21)gcnngc61, 90, 440, 464, 687, 941, 965, 1016, 1033, 11831369, 1588, 1654, 1688, 1733, 1791, 2390, 3223, 3783, 38203906Hpy188III (21)tcnnga77, 379, 924, 1135, 1308, 1624, 1633, 1716, 1772, 18161906, 1939, 1977, 2107, 2366, 3115, 3189, 3272, 3370, 34683602M.AluI (21)agct58, 122, 217, 239, 441, 684, 909, 962, 988, 10341125, 1394, 1852, 2466, 2566, 2629, 3275, 3532, 3578, 36683894M.EsaBC1I (21)agct58, 122, 217, 239, 441, 684, 909, 962, 988, 10341125, 1394, 1852, 2466, 2566, 2629, 3275, 3532, 3578, 36683894BsrI (19)actgg617, 713, 872, 2325, 2443, 2486, 2925, 3419, 3432, 81357, 383, 914, 995, 1079, 1229, 1430, 2750, 3302HpyCH4V (18)tgca55, 274, 419, 620, 667, 966, 1068, 1180, 1339, 15141528, 2331, 2421, 2614, 2702, 2946, 3224, 3520M.CviRI (18)tgca55, 274, 419, 620, 667, 966, 1068, 1180, 1339, 15141528, 2331, 2421, 2614, 2702, 2946, 3224, 3520M.CviSI (18)tgca55, 274, 419, 620, 667, 966, 1068, 1180, 1339, 15141528, 2331, 2421, 2614, 2702, 2946, 3224, 3520AlwI (17)ggatc606, 852, 1540, 2137, 2601, 2922, 3194, 839, 1103, 11201150, 1461, 1707, 2124, 2904, 3182, 3268HaeIII (17)ggcc41, 311, 359, 461, 614, 710, 1196, 1370, 1761, 17882320, 2400, 2667, 3358, 3792, 3810, 3821M.AlwI (17)ggatc606, 852, 1540, 2137, 2601, 2922, 3194, 839, 1103, 11201150, 1461, 1707, 2124, 2904, 3182, 3268M.AvaVII (17)ggcc41, 311, 359, 461, 614, 710, 1196, 1370, 1761, 17882320, 2400, 2667, 3358, 3792, 3810, 3821M.BspRI (17)ggcc41, 311, 359, 461, 614, 710, 1196, 1370, 1761, 17882320, 2400, 2667, 3358, 3792, 3810, 3821M.BsuRI (17)ggcc41, 311, 359, 461, 614, 710, 1196, 1370, 1761, 17882320, 2400, 2667, 3358, 3792, 3810, 3821M.EsaDix4I (17)ttaa51, 110, 248, 281, 296, 409, 514, 787, 1960, 21432148, 2162, 2215, 2450, 2489, 2854, 3129M.EsaDix5I (17)ttaa51, 110, 248, 281, 296, 409, 514, 787, 1960, 21432148, 2162, 2215, 2450, 2489, 2854, 3129M.HaeIII (17)ggcc41, 311, 359, 461, 614, 710, 1196, 1370, 1761, 17882320, 2400, 2667, 3358, 3792, 3810, 3821M.Hpy99VA (17)cctc459, 1147, 1211, 1347, 1704, 2224, 2305, 3663, 3946, 25253, 1045, 1880, 2435, 2641, 3396, 3720M.MnlIA (17)cctc459, 1147, 1211, 1347, 1704, 2224, 2305, 3663, 3946, 25253, 1045, 1880, 2435, 2641, 3396, 3720M.MnlIB (17)cctc459, 1147, 1211, 1347, 1704, 2224, 2305, 3663, 3946, 25253, 1045, 1880, 2435, 2641, 3396, 3720M.MseI (17)ttaa51, 110, 248, 281, 296, 409, 514, 787, 1960, 21432148, 2162, 2215, 2450, 2489, 2854, 3129M.MthTI (17)ggcc41, 311, 359, 461, 614, 710, 1196, 1370, 1761, 17882320, 2400, 2667, 3358, 3792, 3810, 3821M.NgoGII (17)ggcc41, 311, 359, 461, 614, 710, 1196, 1370, 1761, 17882320, 2400, 2667, 3358, 3792, 3810, 3821M.NgoPII (17)ggcc41, 311, 359, 461, 614, 710, 1196, 1370, 1761, 17882320, 2400, 2667, 3358, 3792, 3810, 3821M.Phi3TI (17)ggcc41, 311, 359, 461, 614, 710, 1196, 1370, 1761, 17882320, 2400, 2667, 3358, 3792, 3810, 3821M.Rho11sI (17)ggcc41, 311, 359, 461, 614, 710, 1196, 1370, 1761, 17882320, 2400, 2667, 3358, 3792, 3810, 3821M.SPBetaI (17)ggcc41, 311, 359, 461, 614, 710, 1196, 1370, 1761, 17882320, 2400, 2667, 3358, 3792, 3810, 3821M.SPRI (17)ggcc41, 311, 359, 461, 614, 710, 1196, 1370, 1761, 17882320, 2400, 2667, 3358, 3792, 3810, 3821MnlI (17)cctc459, 1147, 1211, 1347, 1704, 2224, 2305, 3663, 3946, 25253, 1045, 1880, 2435, 2641, 3396, 3720MseI (17)ttaa51, 110, 248, 281, 296, 409, 514, 787, 1960, 21432148, 2162, 2215, 2450, 2489, 2854, 3129BbvI (16)gcagc1250, 1434, 1527, 2330, 3219, 3425, 57, 421, 494, 13511393, 1914, 2696, 3493, 3912, 3930CviAII (16)catg226, 692, 821, 979, 1160, 1505, 1691, 1722, 1748, 21062597, 2607, 2685, 2721, 3114, 3834FatI (16)catg226, 692, 821, 979, 1160, 1505, 1691, 1722, 1748, 21062597, 2607, 2685, 2721, 3114, 3834HpyCH4I (16)catg226, 692, 821, 979, 1160, 1505, 1691, 1722, 1748, 21062597, 2607, 2685, 2721, 3114, 3834M.BbvI (16)gcagc1250, 1434, 1527, 2330, 3219, 3425, 57, 421, 494, 13511393, 1914, 2696, 3493, 3912, 3930M.BbvSI (16)gcwgc57, 421, 494, 1250, 1351, 1393, 1434, 1527, 1914, 23302696, 3219, 3425, 3493, 3912, 3930M.CviAII (16)catg226, 692, 821, 979, 1160, 1505, 1691, 1722, 1748, 21062597, 2607, 2685, 2721, 3114, 3834M.CviQVII (16)catg226, 692, 821, 979, 1160, 1505, 1691, 1722, 1748, 21062597, 2607, 2685, 2721, 3114, 3834M.CviSII (16)catg226, 692, 821, 979, 1160, 1505, 1691, 1722, 1748, 21062597, 2607, 2685, 2721, 3114, 3834M.EsaBS1I (16)catg226, 692, 821, 979, 1160, 1505, 1691, 1722, 1748, 21062597, 2607, 2685, 2721, 3114, 3834M.Hpy166IV (16)catg226, 692, 821, 979, 1160, 1505, 1691, 1722, 1748, 21062597, 2607, 2685, 2721, 3114, 3834M.Hpy188II (16)catg226, 692, 821, 979, 1160, 1505, 1691, 1722, 1748, 21062597, 2607, 2685, 2721, 3114, 3834M.Hpy99X (16)catg226, 692, 821, 979, 1160, 1505, 1691, 1722, 1748, 21062597, 2607, 2685, 2721, 3114, 3834M.HpyAI (16)catg226, 692, 821, 979, 1160, 1505, 1691, 1722, 1748, 21062597, 2607, 2685, 2721, 3114, 3834M.HpyI (16)catg226, 692, 821, 979, 1160, 1505, 1691, 1722, 1748, 21062597, 2607, 2685, 2721, 3114, 3834M.NlaIII (16)catg226, 692, 821, 979, 1160, 1505, 1691, 1722, 1748, 21062597, 2607, 2685, 2721, 3114, 3834NlaIII (16)catg226, 692, 821, 979, 1160, 1505, 1691, 1722, 1748, 21062597, 2607, 2685, 2721, 3114, 3834TseI (16)gcwgc57, 421, 494, 1250, 1351, 1393, 1434, 1527, 1914, 23302696, 3219, 3425, 3493, 3912, 3930Hpy188I (15)tcnga243, 634, 1106, 1129, 1255, 1599, 1952, 2198, 2644, 26552775, 3158, 3292, 3645, 3723HpyF10VI (15)gcnnnnnnngc15, 99, 143, 473, 981, 1281, 1365, 1388, 1527, 16501733, 2399, 3216, 3788, 3902M.BflBF4I (15)gcsgc310, 1095, 1198, 1261, 1356, 1521, 1763, 1859, 1900, 26692791, 3020, 3636, 3791, 3909M.CglASI (15)gcsgc310, 1095, 1198, 1261, 1356, 1521, 1763, 1859, 1900, 26692791, 3020, 3636, 3791, 3909M.DsaV (15)ccngg142, 394, 589, 612, 660, 896, 1293, 1453, 1676, 24612812, 3456, 3674, 3687, 3808M.Ecl18kI (15)ccngg142, 394, 589, 612, 660, 896, 1293, 1453, 1676, 24612812, 3456, 3674, 3687, 3808M.Hpy188I (15)tcnga243, 634, 1106, 1129, 1255, 1599, 1952, 2198, 2644, 26552775, 3158, 3292, 3645, 3723M.NlaX (15)ccngg142, 394, 589, 612, 660, 896, 1293, 1453, 1676, 24612812, 3456, 3674, 3687, 3808M.SenPI (15)ccngg142, 394, 589, 612, 660, 896, 1293, 1453, 1676, 24612812, 3456, 3674, 3687, 3808M.Sse9I (15)aatt108, 183, 200, 246, 288, 304, 319, 324, 351, 10271955, 1971, 2146, 2452, 2707M.SsoII (15)ccngg142, 394, 589, 612, 660, 896, 1293, 1453, 1676, 24612812, 3456, 3674, 3687, 3808MwoI (15)gcnnnnnnngc15, 99, 143, 473, 981, 1281, 1365, 1388, 1527, 16501733, 2399, 3216, 3788, 3902ScrFI (15)ccngg142, 394, 589, 612, 660, 896, 1293, 1453, 1676, 24612812, 3456, 3674, 3687, 3808StyD4I (15)ccngg142, 394, 589, 612, 660, 896, 1293, 1453, 1676, 24612812, 3456, 3674, 3687, 3808TauI (15)gcsgc310, 1095, 1198, 1261, 1356, 1521, 1763, 1859, 1900, 26692791, 3020, 3636, 3791, 3909Tsp509I (15)aatt108, 183, 200, 246, 288, 304, 319, 324, 351, 10271955, 1971, 2146, 2452, 2707M.TspRI (14)castg94, 356, 618, 714, 969, 1413, 2221, 2326, 2673, 27003149, 3420, 3433, 3939TspRI (14)castg94, 356, 618, 714, 969, 1413, 2221, 2326, 2673, 27003149, 3420, 3433, 3939BscAI (13)gcatc1258, 1513, 1729, 1980, 2736, 3747, 1580, 1644, 1919, 20012024, 2526, 2966M.BspST5I (13)gcatc1258, 1513, 1729, 1980, 2736, 3747, 1580, 1644, 1919, 20012024, 2526, 2966SfaNI (13)gcatc1258, 1513, 1729, 1980, 2736, 3747, 1580, 1644, 1919, 20012024, 2526, 2966BstF5I (12)ggatg427, 2259, 2440, 2727, 561, 599, 678, 899, 1082, 11441601, 1626FokI (12)ggatg427, 2259, 2440, 2727, 561, 599, 678, 899, 1082, 11441601, 1626M.BstF5IA (12)ggatg427, 2259, 2440, 2727, 561, 599, 678, 899, 1082, 11441601, 1626M.BstF5ID (12)ggatg427, 2259, 2440, 2727, 561, 599, 678, 899, 1082, 11441601, 1626M.FokI (12)ggatg427, 2259, 2440, 2727, 561, 599, 678, 899, 1082, 11441601, 1626M.HpyAIIA (12)gaaga842, 1949, 2127, 2882, 2960, 3069, 3185, 3337, 457, 7391639, 1849M.MboIIA (12)gaaga842, 1949, 2127, 2882, 2960, 3069, 3185, 3337, 457, 7391639, 1849M.StsI (12)ggatg427, 2259, 2440, 2727, 561, 599, 678, 899, 1082, 11441601, 1626MaeIII (12)gtnac380, 400, 1411, 1717, 2205, 2536, 2594, 2747, 2935, 32993415, 3478MboII (12)gaaga842, 1949, 2127, 2882, 2960, 3069, 3185, 3337, 457, 7391639, 1849StsI (12)ggatg427, 2259, 2440, 2727, 561, 599, 678, 899, 1082, 11441601, 1626BstYI (11)rgatcy840, 852, 1104, 1462, 1708, 2125, 2137, 2905, 2922, 31833194M.BstYI (11)rgatcy840, 852, 1104, 1462, 1708, 2125, 2137, 2905, 2922, 31833194M.NgoMV (11)ggnncc137, 1042, 1290, 1325, 2058, 2228, 2322, 2363, 2574, 37663805M.XhoII (11)rgatcy840, 852, 1104, 1462, 1708, 2125, 2137, 2905, 2922, 31833194NlaIV (11)ggnncc137, 1042, 1290, 1325, 2058, 2228, 2322, 2363, 2574, 37663805BstUI (10)cgcg33, 308, 762, 1354, 1655, 2053, 2342, 2835, 3208, 3789EaeI (10)yggccr42, 312, 360, 615, 711, 1197, 1371, 1762, 1789, 2668Hpy99I (10)cgwcg363, 376, 595, 1376, 1404, 1698, 1713, 2281, 2544, 3734HpyCH4III (10)acngt486, 885, 1668, 1784, 1892, 2202, 2717, 3332, 3802, 3873M.BepI (10)cgcg33, 308, 762, 1354, 1655, 2053, 2342, 2835, 3208, 3789M.BsuEII (10)cgcg33, 308, 762, 1354, 1655, 2053, 2342, 2835, 3208, 3789M.CfrI (10)yggccr42, 312, 360, 615, 711, 1197, 1371, 1762, 1789, 2668M.EaeI (10)yggccr42, 312, 360, 615, 711, 1197, 1371, 1762, 1789, 2668M.EcoHK31I (10)yggccr42, 312, 360, 615, 711, 1197, 1371, 1762, 1789, 2668M.FnuDII (10)cgcg33, 308, 762, 1354, 1655, 2053, 2342, 2835, 3208, 3789SelI (10)cgcg33, 308, 762, 1354, 1655, 2053, 2342, 2835, 3208, 3789BccI (9)ccatc600, 1083, 1109, 1174, 1602, 1502, 2315, 2439, 2726BsaJI (9)ccnngg142, 394, 590, 612, 896, 1100, 1454, 1723, 3675Bsp1286I (9)gdgchc621, 1233, 1326, 1400, 1590, 1652, 2862, 2947, 3521GdiII (9)cggccr42, 312, 360, 1197, 1762, 1789, 2668, 312, 1197HpyAV (9)ccttc1424, 2397, 3009, 3295, 3605, 478, 1925, 1935, 2634HpyCH4IV (9)acgt374, 502, 623, 861, 1405, 1592, 2504, 2877, 3132M.Hpy99XI (9)acgt374, 502, 623, 861, 1405, 1592, 2504, 2877, 3132M.HpyCH4IV (9)acgt374, 502, 623, 861, 1405, 1592, 2504, 2877, 3132TaiI (9)acgt374, 502, 623, 861, 1405, 1592, 2504, 2877, 3132BslI (8)ccnnnnnnngg39, 734, 1271, 1815, 3356, 3635, 3801, 3819EcoHI (8)ccsgg589, 660, 896, 1293, 1453, 2461, 2812, 3456Hin4I (8)gaynnnnnvtc546, 1468, 1498, 2280, 643, 1468, 2280, 2354HinfI (8)gantc46, 340, 1775, 1909, 2274, 3464, 3860, 3935HphI (8)ggtga845, 1476, 2130, 2357, 2979, 603, 2753, 2994M.BabI (8)gantc46, 340, 1775, 1909, 2274, 3464, 3860, 3935M.BcnIA (8)ccsgg589, 660, 896, 1293, 1453, 2461, 2812, 3456M.BcnIB (8)ccsgg589, 660, 896, 1293, 1453, 2461, 2812, 3456M.CcrMI (8)gantc46, 340, 1775, 1909, 2274, 3464, 3860, 3935M.CviBI (8)gantc46, 340, 1775, 1909, 2274, 3464, 3860, 3935M.CviQVI (8)gantc46, 340, 1775, 1909, 2274, 3464, 3860, 3935M.Eco1831I (8)ccsgg589, 660, 896, 1293, 1453, 2461, 2812, 3456M.EcoHI (8)ccsgg589, 660, 896, 1293, 1453, 2461, 2812, 3456M.HhaII (8)gantc46, 340, 1775, 1909, 2274, 3464, 3860, 3935M.HinfI (8)gantc46, 340, 1775, 1909, 2274, 3464, 3860, 3935M.HphIA (8)ggtga845, 1476, 2130, 2357, 2979, 603, 2753, 2994M.HphIB (8)ggtga845, 1476, 2130, 2357, 2979, 603, 2753, 2994M.Hpy99IX (8)gantc46, 340, 1775, 1909, 2274, 3464, 3860, 3935M.HpyAIV (8)gantc46, 340, 1775, 1909, 2274, 3464, 3860, 3935M.NgoBVIIIA (8)ggtga845, 1476, 2130, 2357, 2979, 603, 2753, 2994M.NgoHVIII (8)ggtga845, 1476, 2130, 2357, 2979, 603, 2753, 2994M.SscL1I (8)gantc46, 340, 1775, 1909, 2274, 3464, 3860, 3935M.StyLTI (8)cagag1047, 1128, 1254, 3376, 3402, 3722, 568, 3313NciI (8)ccsgg589, 660, 896, 1293, 1453, 2461, 2812, 3456SspD5I (8)ggtga845, 1476, 2130, 2357, 2979, 603, 2753, 2994TspDTI (8)atgaa48, 1777, 2257, 2560, 680, 901, 1334, 2155BsiEI (7)cgrycg312, 472, 1197, 2647, 2796, 3498, 3922BstNI (7)ccwgg142, 394, 612, 1676, 3674, 3687, 3808CjeI (7)ccannnnnngt3253, 3808, 446, 623, 3154, 3254, 3332EsaBC3I (7)tcga1402, 1558, 1582, 1618, 1780, 2929, 3735Hpy8I (7)gtnnac521, 621, 651, 1076, 2189, 2947, 3521M.BstNI (7)ccwgg142, 394, 612, 1676, 3674, 3687, 3808M.CviBIII (7)tcga1402, 1558, 1582, 1618, 1780, 2929, 3735M.CviQIII (7)tcga1402, 1558, 1582, 1618, 1780, 2929, 3735M.CviSIII (7)tcga1402, 1558, 1582, 1618, 1780, 2929, 3735M.EcoKDcm (7)ccwgg142, 394, 612, 1676, 3674, 3687, 3808M.EcoRII (7)ccwgg142, 394, 612, 1676, 3674, 3687, 3808M.EsaBC3I (7)tcga1402, 1558, 1582, 1618, 1780, 2929, 3735M.EsaDix3I (7)tcga1402, 1558, 1582, 1618, 1780, 2929, 3735M.Hpy8I (7)gtnnac521, 621, 651, 1076, 2189, 2947, 3521M.HpyAIX (7)gtnnac521, 621, 651, 1076, 2189, 2947, 3521M.HpyAX (7)tcga1402, 1558, 1582, 1618, 1780, 2929, 3735M.MvaI (7)ccwgg142, 394, 612, 1676, 3674, 3687, 3808M.Phi3TII (7)tcga1402, 1558, 1582, 1618, 1780, 2929, 3735M.TaqI (7)tcga1402, 1558, 1582, 1618, 1780, 2929, 3735M.TthHB8I (7)tcga1402, 1558, 1582, 1618, 1780, 2929, 3735MspA1I (7)cmgckg59, 442, 1035, 1395, 2913, 3250, 3495PspGI (7)ccwgg142, 394, 612, 1676, 3674, 3687, 3808TaqI (7)tcga1402, 1558, 1582, 1618, 1780, 2929, 3735BceAI (6)acggc361, 542, 1264, 3349, 507, 1700BcefI (6)acggc361, 542, 1264, 3349, 507, 1700BfaI (6)ctag267, 848, 990, 2133, 2468, 3340BseMII (6)ctcag2776, 3151, 3560, 242, 754, 2236BsiHKAI (6)gwgcwc621, 1400, 1590, 2862, 2947, 3521BspCNI (6)ctcag2776, 3151, 3560, 242, 754, 2236CdiI (6)catcg1728, 2931, 1576, 1779, 1920, 2527CjePI (6)ccannnnnnntc686, 2193, 2665, 3302, 624, 3155DdeI (6)ctnag242, 754, 2236, 2776, 3151, 3560FmuI (6)ggncc462, 1806, 2321, 2400, 2417, 2639HaeI (6)wggccw615, 711, 1371, 3359, 3811, 3822M.Cfr13I (6)ggncc462, 1806, 2321, 2400, 2417, 2639M.DdeI (6)ctnag242, 754, 2236, 2776, 3151, 3560M.EcoPI (6)agacc723, 1612, 2196, 1310, 2347, 3160M.MthZI (6)ctag267, 848, 990, 2133, 2468, 3340M.PspPI (6)ggncc462, 1806, 2321, 2400, 2417, 2639M.Sau96I (6)ggncc462, 1806, 2321, 2400, 2417, 2639Sau96I (6)ggncc462, 1806, 2321, 2400, 2417, 2639SmlI (6)ctyrag297, 1682, 2900, 3190, 3467, 3729UnbI (6)ggncc462, 1806, 2321, 2400, 2417, 2639BanI (5)ggyrcc137, 1042, 1290, 1325, 2228Bme1580I (5)gkgcmc621, 1233, 1326, 2947, 3521BmrI (5)actggg2325, 384, 915, 996, 1230BpuEI (5)cttgag1682, 2900, 3467, 3190, 3729BsaWI (5)wccggw1024, 1322, 2571, 3482, 3629BsmAI (5)gtctc1138, 2346, 643, 724, 3111Eco57MI (5)ctgrag2365, 2962, 3308, 1416, 1848M.Alw26I (5)gtctc1138, 2346, 643, 724, 3111M.BfiIA (5)actggg2325, 384, 915, 996, 1230M.BfiIB (5)actggg2325, 384, 915, 996, 1230M.HgiCI (5)ggyrcc137, 1042, 1290, 1325, 2228M.Hpy99II (5)gtsac380, 1411, 1717, 2536, 2747M.Tsp45I (5)gtsac380, 1411, 1717, 2536, 2747MslI (5)caynnnnrtg707, 1728, 2532, 2691, 3050SfcI (5)ctryag275, 334, 2521, 3379, 3570SimI (5)gggtc893, 1719, 2348, 3161, 3642Tsp45I (5)gtsac380, 1411, 1717, 2536, 2747Tth111II (5)caarca555, 1736, 3222, 3261, 3228AcuI (4)ctgaag2962, 3308, 1416, 1848BspMI (4)acctgc277, 958, 1187, 1550BsrBI (4)ccgctc191, 1902, 2103, 3904BsrFI (4)rccggy721, 1610, 1791, 2360BtsI (4)gcagtg2701, 94, 969, 2673Csp6I (4)gtac504, 564, 1594, 2757DraI (4)tttaaa282, 2144, 2163, 2855EarI (4)ctcttc458, 1640, 1850, 3069EciI (4)ggcgga2433, 1804, 3617, 3763FauI (4)cccgc89, 1420, 32, 464HaeII (4)rgcgcy7, 1042, 1290, 3591LpnI (4)rgcgcy7, 1042, 1290, 3591M.AvaIX (4)rccggy721, 1610, 1791, 2360M.Cfr10I (4)rccggy721, 1610, 1791, 2360M.Cfr6I (4)cagctg59, 442, 1035, 1395M.CviQI (4)gtac504, 564, 1594, 2757M.CviRII (4)gtac504, 564, 1594, 2757M.DraI (4)tttaaa282, 2144, 2163, 2855M.Eco57IA (4)ctgaag2962, 3308, 1416, 1848M.Eco57IB (4)ctgaag2962, 3308, 1416, 1848M.EcoP15I (4)cagcag629, 3218, 3427, 3311M.MlyI (4)gastc340, 2274, 3464, 3935M.PvuII (4)cagctg59, 442, 1035, 1395M.RsaI (4)gtac504, 564, 1594, 2757M.TfiI (4)gawtc46, 1775, 1909, 3860MlyI (4)gagtc2274, 3464, 340, 3935PleI (4)gagtc2274, 3464, 340, 3935PvuII (4)cagctg59, 442, 1035, 1395RsaI (4)gtac504, 564, 1594, 2757TaqII (4)gaccga2638, 1312, 2797, 3923TfiI (4)gawtc46, 1775, 1909, 3860TspGWI (4)acgga727, 2729, 597, 3046ApaLI (3)gtgcac621, 2947, 3521AseI (3)attaat52, 111, 2451AvaII (3)ggwcc1806, 2417, 2639BciVI (3)gtatcc1499, 2099, 3626BsaAI (3)yacgtr503, 862, 1593BsaHI (3)grcgyc1042, 1290, 2816BseYI (3)cccagc1037, 1795, 3531BsrDI (3)gcaatg2516, 1517, 2334BssSI (3)cacgag1883, 2944, 3662FspI (3)tgcgca492, 1391, 2500HgaI (3)gacgc2816, 3155, 3733M.BsaAI (3)yacgtr503, 862, 1593M.HgaIA (3)gacgc2816, 3155, 3733M.HgaIB (3)gacgc2816, 3155, 3733M.SinI (3)ggwcc1806, 2417, 2639M.VspI (3)attaat52, 111, 2451MscI (3)tggcca615, 711, 1371Psp03I (3)ggwcc1806, 2417, 2639VpaK11AI (3)ggwcc1806, 2417, 2639AceIII (2)cagctc3895, 2567AclI (2)aacgtt2505, 2878AlwNI (2)cagnnnctg913, 3424BbeI (2)ggcgcc1042, 1290BsaBI (2)gatnnnnatc605, 1150BsaI (2)ggtctc2347, 724BsmFI (2)gggac264, 1427Bsp24I (2)gacnnnnnntgg624, 3155BspHI (2)tcatga2107, 3115DrdI (2)gacnnnnnngtc1318, 3733EagI (2)cggccg312, 1197HaeIV (2)gaynnnnnrtc1468, 2280KasI (2)ggcgcc1042, 1290M.AclI (2)aacgtt2505, 2878M.BspLU11IIIA (2)gggac264, 1427M.BspLU11IIIC (2)gggac264, 1427M.CfrBI (2)ccwwgg1100, 1723M.HaeIV (2)gaynnnnnrtc1468, 2280M.MmeIA (2)tccrac3461, 3645M.MmeIB (2)tccrac3461, 3645M.NgoBIX (2)gtannnnnctc3568, 2941M.NspHI (2)rcatgy1692, 3835M.NspI (2)rcatgy1692, 3835M.PhiMx8I (2)ctssag275, 2365M.XmaIII (2)cggccg312, 1197MmeI (2)tccrac3461, 3645NarI (2)ggcgcc1042, 1290NspI (2)rcatgy1692, 3835PpiI (2)gaacnnnnnctc2927, 3144PvuI (2)cgatcg472, 2647SapI (2)gctcttc1641, 1851SfoI (2)ggcgcc1042, 1290StyI (2)ccwwgg1100, 1723TatI (2)wgtacw565, 2758AflII (1)cttaag297AflIII (1)acrygt3835AhdI (1)gacnnnnngtc2280ApoI (1)raatty320BanII (1)grgcyc1652BcgI (1)cgannnnnntgc2802BclI (1)tgatca1132BglI (1)gccnnnnnggc2399BmgBI (1)cacgtc624BpmI (1)ctggag2365BsaXI (1)acnnnnnctcc28BsiWI (1)cgtacg505BsrGI (1)tgtaca565BstXI (1)ccannnnnntgg708BtgI (1)ccrygg1723FalI (1)aagnnnnnctt1613M.AflIII (1)acrygt3835M.BanII (1)grgcyc1652M.BcgI (1)cgannnnnntgc2802M.BclI (1)tgatca1132M.BmeTI (1)tgatca1132M.BsuBI (1)ctgcag275M.EcoKI (1)aacnnnnnngtgc2945M.EcoR124II (1)gaannnnnnnrtcg1581M.NgoMIV (1)gccggc1791M.NmeSI (1)agtact2758M.PstI (1)ctgcag275M.SauLPI (1)gccggc1791M.ScaI (1)agtact2758M.SnaBI (1)tacgta503M.StyLTIII (1)gagnnnnnnrtayg2787M.StySPI (1)aacnnnnnngtrc2945M.TaeII (1)tgatca1132M.Tth111I (1)gacnnngtc1409M.XcmI (1)ccannnnnnnnntgg1109NaeI (1)gccggc1791NcoI (1)ccatgg1723NgoMIV (1)gccggc1791NotI (1)gcggccgc313PciI (1)acatgt3835PmeI (1)gtttaaac283PstI (1)ctgcag275RsrII (1)cggwccg1807SbfI (1)cctgcagg276ScaI (1)agtact2758SnaBI (1)tacgta503SpeI (1)actagt268SphI (1)gcatgc1692SspI (1)aatatt3082Tth111I (1)gacnnngtc1409XcmI (1)ccannnnnnnnntgg1109XmnI (1)gaannnnttc2879 Non-Cutters AarI, AatII, Acc65I, AccI, AfeI, AgeI, AleI, AloI, ApaBI, ApaI AscI, AsiSI, AvaI, AvrII, BaeI, BamHI, Bbr7I, BbsI, BbvCI, BfrBI BglII, BlpI, BmtI, BplI, Bpu10I, BseRI, BsgI, BsmBI, BsmI, BspEI BssHII, BstAPI, BstBI, BstEII, BstZ17I, Bsu36I, ClaI, DraIII, EcoICRI, EcoNI EcoO109I, EcoRI, EcoRV, FseI, FspAI, HincII, HindIII, HpaI, KpnI, M.AccI M.AloI, M.ApaI, M.AquI, M.BaeI, M.BbvCIA, M.BbvCIB, M.BglII, M.BnaI, M.BseCI, M.Bsp106I M.BstI, M.BstVI, M.Bsu15I, M.Cfr9I, M.ClaI, M.DraIII, M.EcaI, M.EcoAI, M.EcoBI, M.EcoO109I M.EcoR124I, M.EcoRI, M.EcoRV, M.Esp3I, M.HincII, M.HindII, M.HindIII, M.HpaI, M.KpnAI, M.KpnBI M.KpnI, M.MunI, M.NgoAV, M.PaeR7I, M.PhiBssHII, M.PshAI, M.Rrh4273I, M.RsrI, M.SacI, M.SalI M.Sau3239I, M.SmaI, M.SsoI, M.XbaI, M.XcyI, M.XhoI, MfeI, MluI, NdeI, NheI Nli3877I, NruI, NsiI, PacI, PflMI, PfoI, PmlI, Ppu10I, PpuMI, PshAI PsiI, PspOMI, PsrI, PssI, RleAI, SacI, SacII, SalI, SanDI, SciI SexAI, SfiI, SgrAI, SmaI, SrfI, Sse232I, Sse8647I, StuI, SwaI, UthSI XbaI, XhoI, XmaI, ZraI  ') H I J O U V 2:TVҿҮҿ˟ҚqqqqY/hPphr5B*CJOJQJ^JaJo(ph$hPp0J5CJOJQJ^JaJo(!hPp0J5CJOJQJ^JaJ hro( h7o(hG^hSfHq & *hhSfHq & hRo( h#hS hhS hShS ho(hhShqX*hIB*CJKHOJQJ^JaJo(ph ()L6@\]gdr $7$8$H$a$gdr$a$gdPpgdgdS } gdqXgdqX$ 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